LOCUS       sequence-43633-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS7c-RFP
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    147..163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(167..186)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(168..186)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(274..293)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        499..576
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(596..615)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gaccttcacc ttcaccttcg atttcgaact cgtgaccgtt aacggaacct tccatacgaa
       61 ctttgaaacg catgaactct ttgataacgt cttcgctact cgccatatgt atatctcctt
      121 cttaaaagat cttttgaatt ctgaaattgt tatccgctca caattcccct atagtgagtc
      181 gtattaattt cgcgggatcg agatcgatct cgatcctcta cgccggacgc atcgtggccg
      241 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
      301 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
      361 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
      421 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
      481 gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga
      541 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat
      601 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa cca
//
