LOCUS       sequence-436346-       10522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal specific expression of the fusion construct PGK1-HALO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Kokotos AC, Antoniazzi AM, Unda SR, Ko MS, Park D, Eliezer D, 
            Kaplitt MG, De Camilli P, Ryan TA
  TITLE     Phosphoglycerate kinase is a central leverage point in Parkinson's 
            disease-driven neuronal metabolic deficits.
  JOURNAL   Sci Adv. 2024 Aug 23;10(34):eadn6016. doi: 10.1126/sciadv.adn6016. 
            Epub 2024 Aug 21.
  PUBMED    39167658
REFERENCE   2  (bases 1 to 10522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-08-23"; volume: "10(34):eadn6016. doi"; pages: " 
            10.1126/sciadv.adn6016. Epub 2024 Aug 21"
COMMENT     Sequence Label: plenti hSyn PGK1-HALO
FEATURES             Location/Qualifiers
     source          1..10522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2541..2988
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             4323..5204
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     misc_feature    5329..5917
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5382..5402)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5800..5811)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6324..6504
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6530..6547)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6536..6760
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6806..7234
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6893..6912)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7103..7124
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7243..7263)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7248..7577
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7428..7563
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7490..7509
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7625..7672
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7691..8065
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8195..8316
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8232..8251)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8286..8305
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8365..8381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8365..8381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8378..8400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8389..8405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8413..8419,8420..8437,8438..8443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8458..8479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8596..8613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8767..9355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8847..8866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9526..10317,10318..10386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10149..10168
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10387..10491)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gatgctagac
     2521 tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag gcggggtggg
     2581 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2641 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2701 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2761 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc
     2821 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2881 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     2941 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gatctagacg
     3001 ataagggcga attctgcaga tatccatcac actggcggcc gctcgaggcc accatgtcgc
     3061 tttctaacaa gctgacgctg gacaagctgg acgttaaagg gaagcgggtc gttatgagag
     3121 tcgacttcaa tgttcctatg aagaacaacc agataacaaa caaccagagg attaaggctg
     3181 ctgtcccaag catcaaattc tgcttggaca atggagccaa gtcggtagtc cttatgagcc
     3241 acctaggccg gcctgatggt gtgcccatgc ctgacaagta ctccttagag ccagttgctg
     3301 tagaactcaa atctctgctg ggcaaggatg ttctgttctt gaaggactgt gtaggcccag
     3361 aagtggagaa agcctgtgcc aacccagctg ctgggtctgt catcctgctg gagaacctcc
     3421 gctttcatgt ggaggaagaa gggaagggaa aagatgcttc tgggaacaag gttaaagccg
     3481 agccagccaa aatagaagct ttccgagctt cactttccaa gctaggggat gtctatgtca
     3541 atgatgcttt tggcactgct cacagagccc acagctccat ggtaggagtc aatctgccac
     3601 agaaggctgg tgggtttttg atgaagaagg agctgaacta ctttgcaaag gccttggaga
     3661 gcccagagcg acccttcctg gccatcctgg gcggagctaa agttgcagac aagatccagc
     3721 tcatcaataa tatgctggac aaagtcaatg agatgattat tggtggtgga atggctttta
     3781 ccttccttaa ggtgctcaac aacatggaga ttggcacttc tctgtttgat gaagagggag
     3841 ccaagattgt caaagaccta atgtccaaag ctgagaagaa tggtgtgaag attaccttgc
     3901 ctgttgactt tgtcactgct gacaagtttg atgagaatgc caagactggc caagccactg
     3961 tggcttctgg catacctgct ggctggatgg gcttggactg tggtcctgaa agcagcaaga
     4021 agtatgctga ggctgtcact cgggctaagc agattgtgtg gaatggtcct gtgggggtat
     4081 ttgaatggga agcttttgcc cggggaacca aagctctcat ggatgaggtg gtgaaagcca
     4141 cttctagggg ctgcatcacc atcataggtg gtggagacac tgccacttgc tgtgccaaat
     4201 ggaacacgga ggataaagtc agccatgtga gcactggggg tggtgccagt ttggagctcc
     4261 tggaaggtaa agtccttcct ggggtggatg ctctcagcaa tattctcgag catgcatcta
     4321 gagaaatcgg caccggcttc cctttcgacc cccactacgt ggaagtgctg ggcgagagaa
     4381 tgcactatgt ggacgtgggc cctagggacg gaacccccgt gctgtttctg cacggcaacc
     4441 ccaccagcag ctacgtgtgg cggaacatca tcccccacgt ggcccccacc cacagatgta
     4501 tcgcccctga cctgatcggc atgggcaaga gcgacaagcc cgacctgggc tacttcttcg
     4561 acgaccacgt gcggttcatg gacgccttca tcgaggccct gggcctggaa gaggtggtgc
     4621 tcgtgatcca cgattggggc tctgccctgg gcttccactg ggccaagaga aaccccgaga
     4681 gagtgaaggg aatcgccttc atggaattca tcagacccat ccccacctgg gacgagtggc
     4741 ccgagttcgc cagagagaca ttccaggcct tcagaaccac cgacgtgggc agaaagctga
     4801 tcatcgacca gaacgtgttc atcgaaggca ccctgcctat gggcgtcgtg cggcctctga
     4861 ccgaggtgga aatggaccac tacagagagc cctttctgaa ccccgtggac cgcgaacctc
     4921 tgtggcggtt ccctaacgag ctgcctatcg ctggcgagcc cgccaacatt gtggccctgg
     4981 tggaagagta catggactgg ctgcaccaga gccccgtgcc caagctgctg ttttggggaa
     5041 cacccggcgt gctgatccct cctgctgagg ctgctagact ggccaagagc ctgcccaact
     5101 gcaaggccgt ggatatcggc cctggcctga acctgctgca ggaagataac cccgatctga
     5161 tcggctccga gatcgcccgg tggctgagca ccctggaaat cagctaggtt taaacctgga
     5221 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     5281 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcagacaa tcaacctctg
     5341 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5401 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5461 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5521 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5581 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5641 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5701 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     5761 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5821 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5881 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt
     5941 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6001 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     6061 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     6121 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     6181 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     6241 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     6301 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6361 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6421 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6481 agtcagtgtg gaaaatctct agcagggccg ctttaaaccc gctgatcagc ctcgactgtg
     6541 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     6601 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     6661 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     6721 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     6781 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6841 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6901 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6961 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7021 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7081 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7141 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7201 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     7261 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7321 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7381 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     7441 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7501 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7561 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     7621 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     7681 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     7741 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     7801 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     7861 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     7921 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     7981 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     8041 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     8101 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     8161 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     8221 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8281 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     8341 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8401 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8461 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8521 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8581 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8641 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8701 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8761 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8821 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8881 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8941 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9001 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9061 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9121 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9181 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9241 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9301 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9361 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9421 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9481 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9541 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9601 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9661 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9721 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9781 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9841 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9901 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9961 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10021 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10081 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10141 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10441 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10501 ccgaaaagtg ccacctgacg tc
//
