LOCUS       sequence-436348-        4981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal specific expression of mRuby.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Kokotos AC, Antoniazzi AM, Unda SR, Ko MS, Park D, Eliezer D, 
            Kaplitt MG, De Camilli P, Ryan TA
  TITLE     Phosphoglycerate kinase is a central leverage point in Parkinson's 
            disease-driven neuronal metabolic deficits.
  JOURNAL   Sci Adv. 2024 Aug 23;10(34):eadn6016. doi: 10.1126/sciadv.adn6016. 
            Epub 2024 Aug 21.
  PUBMED    39167658
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-08-23"; volume: "10(34):eadn6016. doi"; pages: " 
            10.1126/sciadv.adn6016. Epub 2024 Aug 21"
COMMENT     Sequence Label: AAV hSyn mRuby
FEATURES             Location/Qualifiers
     source          1..4981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   95..224
                     /label=AAV2 ITR
     promoter        306..753
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      781..790
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             787..1497
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    1513..2101
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1566..1586)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1984..1995)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2104..2120)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2105..2121)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    2150..2271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2161..2180)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2215..2234
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2433..2562
                     /label=AAV2 ITR
     rep_origin      complement(2799..3387)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2879..2898)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3558..4349,4350..4418))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4181..4200
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4419..4523)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(4549..4981,1..23))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4686..4707)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4898..4917
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
       61 gcgatcggtg cgggcctctt cgctattacg ccagctgcgc gctcgctcgc tcactgaggc
      121 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
      181 agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa
      241 cccgccatgc tacttatcta cgtagccatg ctctaggaag atctctgcag agggccctgc
      301 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      361 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      421 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      481 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      541 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      601 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      661 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      721 agcggaggag tcgtgtcgtg cctgagagcg cagtcgaatt caagctgcta gcaaggatcc
      781 gccaccatgg tgtctaaggg cgaagagctg atcaaggaaa atatgcgtat gaaggtggtc
      841 atggaaggtt cggtcaacgg ccaccaattc aaatgcacag gtgaaggaga aggcagaccg
      901 tacgagggag tgcaaaccat gaggatcaaa gtcatcgagg gaggacccct gccatttgcc
      961 tttgacattc ttgccacgtc gttcatgtat ggcagccgta cctttatcaa gtacccggcc
     1021 gacatccctg atttctttaa acagtccttt cctgagggtt ttacttggga aagagttacg
     1081 agatacgaag atggtggagt cgtcaccgtc acgcaggaca ccagccttga ggatggcgag
     1141 ctcgtctaca acgtcaaggt cagaggggta aactttccct ccaatggtcc cgtgatgcag
     1201 aagaagacca agggttggga gcctaataca gagatgatgt atccagcaga tggtggtctg
     1261 agaggataca ctgacatcgc actgaaagtt gatggtggtg gccatctgca ctgcaacttc
     1321 gtgacaactt acaggtcaaa aaagaccgtc gggaacatca agatgcccgg tgtccatgcc
     1381 gttgatcacc gcctggaaag gatcgaggag agtgacaatg aaacctacgt agtgcaaaga
     1441 gaagtggcag ttgccaaata cagcaacctt ggtggtggca tggacgagct gtacaagtag
     1501 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     1561 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     1621 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     1681 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     1741 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     1801 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     1861 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     1921 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     1981 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     2041 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     2101 catcgatacc gtcgacctcg acccgggcgg ccgcttcgag cagacatgat aagatacatt
     2161 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     2221 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     2281 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag
     2341 taaaacctct acaaatgtgg taaaatcgat aaggatcttc ctagagcatg gctacgtaga
     2401 taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac
     2461 tccctctctg cgcgcccgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     2521 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcatta atgaatcggc
     2581 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2641 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2701 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2761 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2821 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2881 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2941 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3001 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3061 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3121 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3181 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3241 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3301 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3361 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3421 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3541 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3601 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3661 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3721 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3781 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3841 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3901 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3961 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4021 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4081 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4141 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4201 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4261 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4321 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4381 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4441 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4501 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     4561 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     4621 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     4681 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     4741 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     4801 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     4861 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     4921 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     4981 t
//
