LOCUS       sequence-436351-       11122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal specific expression of HK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11122)
  AUTHORS   Kokotos AC, Antoniazzi AM, Unda SR, Ko MS, Park D, Eliezer D, 
            Kaplitt MG, De Camilli P, Ryan TA
  TITLE     Phosphoglycerate kinase is a central leverage point in Parkinson's 
            disease-driven neuronal metabolic deficits.
  JOURNAL   Sci Adv. 2024 Aug 23;10(34):eadn6016. doi: 10.1126/sciadv.adn6016. 
            Epub 2024 Aug 21.
  PUBMED    39167658
REFERENCE   2  (bases 1 to 11122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-08-23"; volume: "10(34):eadn6016. doi"; pages: " 
            10.1126/sciadv.adn6016. Epub 2024 Aug 21"
COMMENT     Sequence Label: plenti hSyn HK1
FEATURES             Location/Qualifiers
     source          1..11122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2541..2988
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    5929..6517
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5982..6002)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6400..6411)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6924..7104
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7130..7147)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7136..7360
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7406..7834
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7493..7512)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7703..7724
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7843..7863)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7848..8177
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8028..8163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8090..8109
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8225..8272
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8291..8665
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8795..8916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8832..8851)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8886..8905
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8965..8981)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8965..8981)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8978..9000)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8989..9005
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9013..9019,9020..9037,9038..9043))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9058..9079
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9196..9213)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9367..9955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9447..9466)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10126..10917,10918..10986))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10987..11091)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gatgctagac
     2521 tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag gcggggtggg
     2581 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2641 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2701 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2761 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc
     2821 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2881 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     2941 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gatctagacg
     3001 ataagggcga attctgcaga tatccatcac actggcggcc gctcgaggcc accatgatcg
     3061 ccgcgcagct cctggcctat tacttcacgg agctgaagga tgaccaggtc aaaaagattg
     3121 acaagtatct gtatgccatg cggctctccg atgaaactct catagatatc atgactcgct
     3181 tcaggaagga gatgaagaat ggcctctccc gggattttaa tccaacagcc acagtcaaga
     3241 tgttgccaac attcgtaagg tccattcctg atggctctga aaagggagat ttcattgccc
     3301 tggatcttgg tgggtcttcc tttcgaattc tgcgggtgca agtgaatcat gagaaaaacc
     3361 agaatgttca catggagtcc gaggtttatg acaccccaga gaacatcgtg cacggcagtg
     3421 gaagccagct ttttgatcat gttgctgagt gcctgggaga tttcatggag aaaaggaaga
     3481 tcaaggacaa gaagttacct gtgggattca cgttttcttt tccttgccaa caatccaaaa
     3541 tagatgaggc catcctgatc acctggacaa agcgatttaa agcgagcgga gtggaaggag
     3601 cagatgtggt caaactgctt aacaaagcca tcaaaaagcg aggggactat gatgccaaca
     3661 tcgtagctgt ggtgaatgac acagtgggca ccatgatgac ctgtggctat gacgaccagc
     3721 actgtgaagt cggcctgatc atcggcactg gcaccaatgc ttgctacatg gaggaactga
     3781 ggcacattga tctggtggaa ggagacgagg ggaggatgtg tatcaataca gaatggggag
     3841 cctttggaga cgatggatca ttagaagaca tccggacaga gtttgacagg gagatagacc
     3901 ggggatccct caaccctgga aaacagctgt ttgagaagat ggtcagtggc atgtacttgg
     3961 gagagctggt tcgactgatc ctagtcaaga tggccaagga gggcctctta tttgaagggc
     4021 ggatcacccc ggagctgctc acccgaggga agtttaacac cagtgatgtg tcagccatcg
     4081 aaaagaataa ggaaggcctc cacaatgcca aagaaatcct gacccgcctg ggagtggagc
     4141 cgtccgatga tgactgtgtc tcagtccagc acgtttgcac cattgtctca tttcgctcag
     4201 ccaacttggt ggctgccaca ctgggcgcca tcttgaaccg cctgcgtgat aacaagggca
     4261 cacccaggct gcggaccacg gttggtgtcg acggatctct ttacaagacg cacccacagt
     4321 attcccggcg tttccacaag actctaaggc gcttggtgcc agactccgat gtgcgcttcc
     4381 tcctctcgga gagtggcagc ggcaaggggg ctgccatggt gacggcggtg gcctaccgct
     4441 tggccgagca gcaccggcag atagaggaga ccctggctca tttccacctc accaaagaca
     4501 tgctgctgga ggtgaagaag aggatgcggg ccgagatgga gctggggctg aggaagcaga
     4561 cgcacaacaa tgccgtggtt aagatgctgc cctccttcgt ccggagaact cccgacggga
     4621 ccgagaatgg tgacttcttg gccctggatc ttggaggaac caatttccgt gtgctgctgg
     4681 tgaaaatccg tagtgggaaa aagagaacgg tggaaatgca caacaagatc tacgccattc
     4741 ctattgaaat catgcagggc actggggaag agctgtttga tcacattgtc tcctgcatct
     4801 ctgacttctt ggactacatg gggatcaaag gccccaggat gcctctgggc ttcacgttct
     4861 catttccctg ccagcagacg agtctggacg cgggaatctt gatcacgtgg acaaagggtt
     4921 ttaaggcaac agactgcgtg ggccacgatg tagtcacctt actaagggat gcgataaaaa
     4981 ggagagagga atttgacctg gacgtggtgg ctgtggtcaa cgacacagtg ggcaccatga
     5041 tgacctgtgc ttatgaggag cccacctgtg aggttggact cattgttggg accggcagca
     5101 atgcctgcta catggaggag atgaagaacg tggagatggt ggagggggac caggggcaga
     5161 tgtgcatcaa catggagtgg ggggcctttg gggacaacgg gtgtctggat gatatcagga
     5221 cacactacga cagactggtg gacgaatatt ccctaaatgc tgggaaacaa aggtatgaga
     5281 agatgatcag tggtatgtac ctgggtgaaa tcgtccgcaa catcttaatc gacttcacca
     5341 agaagggatt cctcttccga gggcagatct ctgagacgct gaagacccgg ggcatctttg
     5401 agaccaagtt tctctctcag atcgagagtg accgattagc actgctccag gtccgggcta
     5461 tcctccagca gctaggtctg aatagcacct gcgatgacag tatcctcgtc aagacagtgt
     5521 gcggggtggt gtccaggagg gccgcacagc tgtgtggcgc aggcatggct gcggttgtgg
     5581 ataagatccg cgagaacaga ggactggacc gtctgaatgt gactgtggga gtggacggga
     5641 cactctacaa gcttcatcca cacttctcca gaatcatgca ccagacggtg aaggaactgt
     5701 caccaaaatg taacgtgtcc ttcctcctgt ctgaggatgg cagcggcaag ggggccgccc
     5761 tcatcacggc cgtgggcgtg cggttacgca cagaggcaag cagctaagtt taaacctgga
     5821 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     5881 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcagacaa tcaacctctg
     5941 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6001 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6061 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6121 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6181 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6241 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6301 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     6361 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6421 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6481 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt
     6541 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6601 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     6661 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     6721 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     6781 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     6841 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     6901 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6961 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7021 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7081 agtcagtgtg gaaaatctct agcagggccg ctttaaaccc gctgatcagc ctcgactgtg
     7141 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7201 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7261 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7321 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     7381 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7441 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7501 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7561 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7621 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7681 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7741 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7801 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     7861 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7921 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7981 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     8041 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8101 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8161 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     8221 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     8281 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     8341 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     8401 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     8461 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     8521 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     8581 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     8641 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     8701 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     8761 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     8821 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8881 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     8941 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     9001 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9061 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9121 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9181 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9241 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9301 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9361 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9421 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9481 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9541 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9601 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9661 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9721 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9781 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9841 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9901 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9961 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10021 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10081 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10141 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10201 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10261 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10321 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10381 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10441 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10501 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10561 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10621 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10681 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10741 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10801 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10861 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10921 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10981 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11041 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11101 ccgaaaagtg ccacctgacg tc
//
