LOCUS       sequence-436369-        9726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal specific expression of PGK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Kokotos AC, Antoniazzi AM, Unda SR, Ko MS, Park D, Eliezer D, 
            Kaplitt MG, De Camilli P, Ryan TA
  TITLE     Phosphoglycerate kinase is a central leverage point in Parkinson's 
            disease-driven neuronal metabolic deficits.
  JOURNAL   Sci Adv. 2024 Aug 23;10(34):eadn6016. doi: 10.1126/sciadv.adn6016. 
            Epub 2024 Aug 21.
  PUBMED    39167658
REFERENCE   2  (bases 1 to 9726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-08-23"; volume: "10(34):eadn6016. doi"; pages: " 
            10.1126/sciadv.adn6016. Epub 2024 Aug 21"
COMMENT     Sequence Label: plenti hSyn PGK1
FEATURES             Location/Qualifiers
     source          1..9726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2541..2988
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    4533..5121
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4586..4606)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5004..5015)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5528..5708
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5734..5751)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5740..5964
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6010..6438
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6097..6116)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6307..6328
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6447..6467)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6452..6781
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6632..6767
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6694..6713
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6829..6876
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6895..7269
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7399..7520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7436..7455)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7490..7509
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7569..7585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7569..7585)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7582..7604)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7593..7609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7617..7623,7624..7641,7642..7647))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7662..7683
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7800..7817)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7971..8559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8051..8070)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8730..9521,9522..9590))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9353..9372
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9591..9695)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gatgctagac
     2521 tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag gcggggtggg
     2581 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2641 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2701 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2761 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc
     2821 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2881 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     2941 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gatctagacg
     3001 ataagggcga attctgcaga tatccatcac actggcggcc gctcgagaga tccgctagcg
     3061 ctaccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt accatgtcgc
     3121 tttctaacaa gctgacgctg gacaagctgg acgttaaagg gaagcgggtc gttatgagag
     3181 tcgacttcaa tgttcctatg aagaacaacc agataacaaa caaccagagg attaaggctg
     3241 ctgtcccaag catcaaattc tgcttggaca atggagccaa gtcggtagtc cttatgagcc
     3301 acctaggccg gcctgatggt gtgcccatgc ctgacaagta ctccttagag ccagttgctg
     3361 tagaactcaa atctctgctg ggcaaggatg ttctgttctt gaaggactgt gtaggcccag
     3421 aagtggagaa agcctgtgcc aacccagctg ctgggtctgt catcctgctg gagaacctcc
     3481 gctttcatgt ggaggaagaa gggaagggaa aagatgcttc tgggaacaag gttaaagccg
     3541 agccagccaa aatagaagct ttccgagctt cactttccaa gctaggggat gtctatgtca
     3601 atgatgcttt tggcactgct cacagagccc acagctccat ggtaggagtc aatctgccac
     3661 agaaggctgg tgggtttttg atgaagaagg agctgaacta ctttgcaaag gccttggaga
     3721 gcccagagcg acccttcctg gccatcctgg gcggagctaa agttgcagac aagatccagc
     3781 tcatcaataa tatgctggac aaagtcaatg agatgattat tggtggtgga atggctttta
     3841 ccttccttaa ggtgctcaac aacatggaga ttggcacttc tctgtttgat gaagagggag
     3901 ccaagattgt caaagaccta atgtccaaag ctgagaagaa tggtgtgaag attaccttgc
     3961 ctgttgactt tgtcactgct gacaagtttg atgagaatgc caagactggc caagccactg
     4021 tggcttctgg catacctgct ggctggatgg gcttggactg tggtcctgaa agcagcaaga
     4081 agtatgctga ggctgtcact cgggctaagc agattgtgtg gaatggtcct gtgggggtat
     4141 ttgaatggga agcttttgcc cggggaacca aagctctcat ggatgaggtg gtgaaagcca
     4201 cttctagggg ctgcatcacc atcataggtg gtggagacac tgccacttgc tgtgccaaat
     4261 ggaacacgga ggataaagtc agccatgtga gcactggggg tggtgccagt ttggagctcc
     4321 tggaaggtaa agtccttcct ggggtggatg ctctcagcaa tatttaggga tccaccggat
     4381 ctagataact gatcataatc agccatacca cgtttaaacc tggaaaaaca tggagcaatc
     4441 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     4501 gaggaggtgg gttttccagt cacacctcag acaatcaacc tctggattac aaaatttgtg
     4561 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4621 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4681 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4741 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4801 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4861 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4921 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4981 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5041 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5101 ccctttgggc cgcctccccg cctggaattc gagctcggta cctttaagac caatgactta
     5161 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5221 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     5281 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     5341 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     5401 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     5461 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     5521 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5581 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5641 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5701 ctctagcagg gccgctttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     5761 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5821 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5881 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     5941 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     6001 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6061 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6121 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6181 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6241 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6301 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6361 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6421 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     6481 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     6541 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6601 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6661 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6721 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6781 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     6841 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     6901 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     6961 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     7021 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     7081 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     7141 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     7201 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     7261 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     7321 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     7381 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7441 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7501 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     7561 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7621 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7681 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7741 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7801 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7861 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7921 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7981 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8041 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8101 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8161 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8221 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8281 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8341 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8401 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8461 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8521 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8581 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8641 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8701 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8761 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8821 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8881 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8941 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9001 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9061 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9121 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9181 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9241 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9301 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9361 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9421 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9481 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9541 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9601 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9661 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9721 gacgtc
//
