LOCUS       sequence-43637-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE2a-RFP
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        202..257
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    203..221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(349..368)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ggcagcggac cacctttggt aactttcagt ttagcggtct gggtaccttc gtacggacga
       61 ccttcacctt caccttcgat ttcgaactcg tgaccgttaa cggaaccttc catacgaact
      121 ttgaaacgca tgaactcttt gataacgtct tcgctactcg ccatatgtat atctccttct
      181 taaaagatct tttgaattct tttctctatc actgataggg agtggtaaaa taactctatc
      241 aacgatagag tgtcaacaaa aattaggaat taatgatgtc tagattagat aaaagtaaag
      301 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
      361 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
      421 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
      481 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
      541 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
      601 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
      661 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
      721 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
      781 tattacgaca agctatcgaa ttatttgatc acc
//
