LOCUS       sequence-436370-       10062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal specific expression of PKM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Kokotos AC, Antoniazzi AM, Unda SR, Ko MS, Park D, Eliezer D, 
            Kaplitt MG, De Camilli P, Ryan TA
  TITLE     Phosphoglycerate kinase is a central leverage point in Parkinson's 
            disease-driven neuronal metabolic deficits.
  JOURNAL   Sci Adv. 2024 Aug 23;10(34):eadn6016. doi: 10.1126/sciadv.adn6016. 
            Epub 2024 Aug 21.
  PUBMED    39167658
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-08-23"; volume: "10(34):eadn6016. doi"; pages: " 
            10.1126/sciadv.adn6016. Epub 2024 Aug 21"
COMMENT     Sequence Label: plenti hSyn PKM1
FEATURES             Location/Qualifiers
     source          1..10062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2541..2988
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    4869..5457
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4922..4942)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5340..5351)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5864..6044
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6070..6087)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6076..6300
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6346..6774
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6433..6452)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6643..6664
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6783..6803)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6788..7117
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6968..7103
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7030..7049
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7165..7212
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7231..7605
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7735..7856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7772..7791)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7826..7845
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7905..7921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7905..7921)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7918..7940)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7929..7945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7953..7959,7960..7977,7978..7983))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7998..8019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8136..8153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8307..8895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8387..8406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9066..9857,9858..9926))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9689..9708
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9927..10031)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gatgctagac
     2521 tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag gcggggtggg
     2581 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2641 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2701 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2761 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc
     2821 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2881 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     2941 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gatctagacg
     3001 ataagggcga attctgcaga tatccatcac actggcggcc gctcgagaga tccgctagcg
     3061 ctaccggact cagatctcga gctcaagctt cgaattctgc agtcgacatg tcgaagcccc
     3121 atagtgaagc cgggactgcc ttcattcaga cccagcagct gcacgcagcc atggctgaca
     3181 cattcctgga gcacatgtgc cgcctggaca ttgattcacc acccatcaca gcccggaaca
     3241 ctggcatcat ctgtaccatt ggcccagctt cccgatcagt ggagacgttg aaggagatga
     3301 ttaagtctgg aatgaatgtg gctcgtctga acttctctca tggaactcat gagtaccatg
     3361 cggagaccat caagaatgtg cgcacagcca cggaaagctt tgcttctgac cccatcctct
     3421 accggcccgt tgctgtggct ctagacacta aaggacctga gatccgaact gggctcatca
     3481 agggcagcgg cactgcagag gtggagctga agaagggagc cactctcaaa atcacgctgg
     3541 ataacgccta catggaaaag tgtgacgaga acatcctgtg gctggactac aagaacatct
     3601 gcaaggtggt ggaagtgggc agcaagatct acgtggatga tgggcttatt tctctccagg
     3661 tgaagcagaa aggtgccgac ttcctggtga cggaggtgga aaatggtggc tccttgggca
     3721 gcaagaaggg tgtgaacctt cctggggctg ctgtggactt gcctgctgtg tcggagaagg
     3781 acatccagga tctgaagttt ggggtcgagc aggatgttga tatggtgttt gcgtcattca
     3841 tccgcaaggc atctgatgtc catgaagtta ggaaggtcct gggagagaag ggaaagaaca
     3901 tcaagattat cagcaaaatc gagaatcatg agggggttcg gaggtttgat gaaatcctgg
     3961 aggccagtga tgggatcatg gtggctcgtg gtgatctagg cattgagatt cctgcagaga
     4021 aggtcttcct tgctcagaag atgatgattg gacggtgcaa ccgagctggg aagcctgtca
     4081 tctgtgctac tcagatgctg gagagcatga tcaagaagcc ccgccccact cgggctgaag
     4141 gcagtgatgt ggccaatgca gtcctggatg gagccgactg catcatgctg tctggagaaa
     4201 cagccaaagg ggactatcct ctggaggctg tgcgcatgca gcacctgata gctcgtgagg
     4261 ctgaggcagc catgttccac cgcaagctgt ttgaagaact tgtgcgagcc tcaagtcact
     4321 ccacagacct catggaagcc atggccatgg gcagcgtgga ggcttcttat aagtgtttag
     4381 cagcagcttt gatagttctg acggagtctg gcaggtctgc tcaccaggtg gccagatacc
     4441 gcccacgtgc ccccatcatt gctgtgaccc ggaatcccca gacagctcgt caggcccacc
     4501 tgtaccgtgg catcttccct gtgctgtgca aggacccagt ccaggaggcc tgggctgagg
     4561 acgtggacct ccgggtgaac tttgccatga atgttggcaa ggcccgaggc ttcttcaaga
     4621 agggagatgt ggtcattgtg ctgaccggat ggcgccctgg ctccggcttc accaacacca
     4681 tgcgtgttgt tcctgtgccg tgaggatcca ccggatctag ataactgatc ataatcagcc
     4741 ataccacgtt taaacctgga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     4801 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     4861 cctcagacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4921 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4981 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5041 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5101 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5161 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5221 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     5281 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5341 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5401 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     5461 gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5521 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     5581 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     5641 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     5701 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     5761 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     5821 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     5881 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5941 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6001 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccg ctttaaaccc
     6061 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6121 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6181 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6241 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6301 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6361 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6421 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6481 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6541 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6601 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6661 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6721 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     6781 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6841 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6901 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6961 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7021 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     7081 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     7141 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     7201 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     7261 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     7321 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     7381 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     7441 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     7501 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     7561 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     7621 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7681 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     7741 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7801 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7861 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7921 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7981 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8041 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8101 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8161 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8221 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8281 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8341 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8401 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8461 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8521 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8581 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8641 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8701 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8761 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8821 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8881 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8941 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9001 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9061 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9121 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9181 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9241 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9301 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9361 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9421 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9481 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9541 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9601 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9661 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9721 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9781 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9841 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9901 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9961 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10021 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
