LOCUS       sequence-436379-        4056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC37A2_STOP
FEATURES             Location/Qualifiers
     source          1..4056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2181..2280)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2297..2316)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2298..2316)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2321..2337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2321..2337)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2439..2458)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2450..3259
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2517..2536)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3406..3994
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3895..3914
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgcgga gctccctggc accaggcgtg tggttctttc gggccttcag
      721 cagagactcc tggtttagag gcctgatcct gctgctgacc ttcctgatct acgcctgcta
      781 tcacatgtcc cggaagccca tcagcatcgt gaagtccaga ctgcaccaga actgttctga
      841 gcagatcaag ccaatcaacg acacccactc cctgaatgat acaatgtggt gctcttgggc
      901 ccccttcgac aaggataact acaaggagct gctgggcggc gtggataatg cctttctgat
      961 cgcctatgcc atcggcatgt tcatcagcgg cgtgtttggc gagaggctgc ctctgcgcta
     1021 ctatctgtcc gccggcatgc tgctgtctgg cctgttcaca agcctgtttg gcctgggcta
     1081 cttctggaac atccacgagc tgtggtattt tgtggtcatc caggtgtgca atggcctggt
     1141 gcagaccaca ggatggccaa gcgtggtgac ctgcgtggga aactggttcg gcaagggcaa
     1201 gcggggcttt atcatgggca tctggaactc tcacacaagc gtgggcaata tcctgggctc
     1261 tctgatcgcc ggcatctggg tgaatggcca gtggggcctg agctttatcg tgcctggcat
     1321 catcaccgcc gtgatgggcg tgatcacatt cctgtttctg atcgagcacc cagaggacgt
     1381 ggattgtgca ccacctcagc accacggaga gccagcagag aaccaggaca atccagagga
     1441 tcccggcaac tccccttgct ctatcaggga gtccggcctg gagaccgtgg ccaagtgctc
     1501 taagggccct tgtgaggagc cagccgccat cagcttcttt ggcgccctga ggatccctgg
     1561 agtggtggag ttcagcctgt gcctgctgtt tgccaagctg gtgtcctaca cattcctgta
     1621 ttggctgcca ctgtacatcg ccaatgtggc acacttcagc gccaaggagg caggcgacct
     1681 gtccaccctg tttgatgtgg gaggaatcat cggaggaatc gtggcaggac tggtgtccga
     1741 ctatacaaac ggcagagcca ccacatgctg cgtgatgctg atcctggccg cccccatgat
     1801 gtttctgtac aattatatcg gccaggatgg catcgcctct agcatcgtga tgctgatcat
     1861 ctgcggagga ctggtgaacg gaccttacgc actgatcacc acagccgtgt ctgccgacct
     1921 gggaacccac aagagcctga agggcaatgc caaggccctg tctaccgtga cagccatcat
     1981 cgatggcacc ggcagcatcg gagccgccct gggaccactg ctggcaggcc tgatctcccc
     2041 tacaggctgg aacaacgtgt tctacatgct gatctctgcc gacgtgctgg catgcctgct
     2101 gctgtgcagg ctggtgtaca aggagatcct ggcctggaag gtgtccctgt ctcgcggctc
     2161 cggctataag gagatctaag acccagcttt cttgtacaaa gttggcatta taagaaagca
     2221 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2281 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2341 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2401 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     2461 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     2521 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     2581 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     2641 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     2701 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     2761 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     2821 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     2881 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     2941 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     3001 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     3061 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     3121 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     3181 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     3241 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     3301 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     3361 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3421 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3481 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3541 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3601 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3661 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3721 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3781 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3841 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3901 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3961 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4021 gttcctggcc ttttgctggc cttttgctca catgtt
//
