LOCUS       sequence-43639-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE2c-RFP
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        191..246
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    192..210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(338..357)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 acctttggta actttcagtt tagcggtctg ggtaccttcg tacggacgac cttcaccttc
       61 accttcgatt tcgaactcgt gaccgttaac ggaaccttcc atacgaactt tgaaacgcat
      121 gaactctttg ataacgtctt cgctactcgc catatgtata tctccttctt aaaagatctt
      181 ttgaattctt ttctctatca ctgataggga gtggtaaaat aactctatca acgatagagt
      241 gtcaacaaaa attaggaatt aatgatgtct agattagata aaagtaaagt gattaacagc
      301 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
      361 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
      421 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
      481 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
      541 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
      601 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
      661 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
      721 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
      781 gctatcgaat tatttgatca
//
