LOCUS       sequence-43641-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE2k-RFP
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        190..245
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    191..209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(337..356)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 cctttggtaa ctttcagttt agcggtctgg gtaccttcgt acggacgacc ttcaccttca
       61 ccttcgattt cgaactcgtg accgttaacg gaaccttcca tacgaacttt gaaacgcatg
      121 aactctttga taacgtcttc gctactcgcc atatgtatat ctccttctta aaagatcttt
      181 tgaattcttt tctctatcac tgatagggag tggtaaaata actctatcaa cgatagagtg
      241 tcaacaaaaa ttaggaatta atgatgtcta gattagataa aagtaaagtg attaacagcg
      301 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
      361 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
      421 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
      481 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
      541 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
      601 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
      661 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
      721 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
      781 ctatcgaatt atttgatca
//
