LOCUS       sequence-43643-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA2a-RFP
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        189..244
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    190..208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(336..355)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ctttggtaac tttcagttta gcggtctggg taccttcgta cggacgacct tcaccttcac
       61 cttcgatttc gaactcgtga ccgttaacgg aaccttccat acgaactttg aaacgcatga
      121 actctttgat aacgtcttcg ctactcgcca tatgtatatc tccttcttaa aagatctttt
      181 gaattctttt ctctatcact gatagggagt ggtaaaataa ctctatcaac gatagagtgt
      241 caacaaaaat taggaattaa tgatgtctag attagataaa agtaaagtga ttaacagcgc
      301 attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa
      361 gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga
      421 cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga
      481 aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca
      541 tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct
      601 cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc
      661 actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt
      721 cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc
      781 tatcgaatta tttgatc
//
