LOCUS       sequence-43645-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA2c-RFP
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        178..233
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    179..197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(325..344)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ttcagtttag cggtctgggt accttcgtac ggacgacctt caccttcacc ttcgatttcg
       61 aactcgtgac cgttaacgga accttccata cgaactttga aacgcatgaa ctctttgata
      121 acgtcttcgc tactcgccat atgtatatct ccttcttaaa agatcttttg aattcttttc
      181 tctatcactg atagggagtg gtaaaataac tctatcaacg atagagtgtc aacaaaaatt
      241 aggaattaat gatgtctaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc
      301 ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag
      361 agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca
      421 ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa agctggcaag
      481 attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag
      541 caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat
      601 tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg
      661 tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag
      721 aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct atcgaattat
      781 ttgatcacca
//
