LOCUS       sequence-43646-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA2k-RFP
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      151..222
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      238..265
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(293..310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(507..526)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
       61 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaagg
      121 atccaaactc gagtaaggat ctccaggcat caaataaaac gaaaggctca gtcgaaagac
      181 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      241 tcaccttcgg gtgggccttt ctgcgtttat acctagggat atattccgct tcctcgctca
      301 ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg
      361 gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa
      421 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
      481 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct
      541 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
      601 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
      661 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
      721 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
      781 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
      841 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
      901 gaaaaacc
//
