LOCUS       sequence-436460-        8353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, 
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, 
            Bonneau R, Selbach M, Dieterich C, Landthaler M
  TITLE     The mRNA-Bound Proteome and Its Global Occupancy Profile on 
            Protein-Coding Transcripts.
  JOURNAL   Mol Cell. 2012 Jun 8;46(5):674-90.
  PUBMED    22681889
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2012-06-8"; volume: "46(5)"; pages: "674-90"
COMMENT     Sequence Label: pFRT/TO/HIS/FLAG/HA-KIAA1967
FEATURES             Location/Qualifiers
     source          1..8353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             67..108
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(165..189)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             242..283
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(363..380)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    369..593
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(726..745)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    877..924
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             932..1954
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             932..1954
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    2084..2205
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2121..2140)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2175..2194
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2254..2270)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2254..2270)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2267..2289)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2278..2294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2302..2308,2309..2326,2327..2332))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2347..2368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2485..2502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2656..3244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2736..2755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3415..4206,4207..4275))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4038..4057
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4276..4380)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4455..4474)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4646..5025
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5026..5229
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5180..5200
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    5231..5249
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5252..5270
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5280..5304
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5404..5427
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             5431..5454
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5455..5481
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    5497..5521
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(8343..8353,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggttgatat ccagcacagt ggcggccgct caagtctaga gggcccgcgg
       61 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg tacctgatcc
      121 gcggccgcat agataactga tccagtgtgc ctcgagatat ctagacccag ctttcttgta
      181 caaagtggtt gatatccagc acagtggcgg ccgctcaagt ctagagggcc cgcggttcga
      241 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtacct gatccgcggc
      301 cgcatagata actgatccag tgtgcctcga gtctagaggg cccgtttaaa cccgctgatc
      361 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
      421 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
      481 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
      541 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
      601 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
      661 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      721 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      781 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
      841 caaaaaactt gattagggtg atggttcacg tacctagaag ttcctattcc gaagttccta
      901 ttctctagaa agtataggaa cttccttggc caaaaagcct gaactcaccg cgacgtctgt
      961 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     1021 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     1081 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     1141 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     1201 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     1261 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     1321 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     1381 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     1441 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     1501 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     1561 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     1621 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     1681 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     1741 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     1801 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     1861 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     1921 acgccccagc actcgtccga gggcaaagga atagcacgta ctacgagatt tcgattccac
     1981 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     2041 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     2101 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     2161 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     2221 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     2281 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     2341 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     2401 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2461 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2521 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2581 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2641 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2701 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2761 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2821 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2881 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2941 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3001 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3061 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3121 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3181 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3241 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3301 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3361 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3421 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3481 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3541 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3601 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3661 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3721 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3781 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3841 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3901 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     3961 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4021 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4081 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4141 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4201 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4261 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4321 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4381 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     4441 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     4501 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     4561 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     4621 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     4681 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     4741 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     4801 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     4861 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     4921 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     4981 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     5041 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     5101 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     5161 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     5221 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
     5281 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     5341 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttggtacc
     5401 atgcaccacc accaccacca ccaccacatg gactacaagg acgacgatga caagtaccct
     5461 tatgacgtgc ccgattacgc tttgaaccag atatcaacaa gtttgtacaa aaaagcaggc
     5521 tccaccatgg gaaccaattc agtcgacatg tcccagttta agcgccagcg gatcaacccg
     5581 cttccagggg gacgcaactt ctcaggcaca gcttcaacat ctcttctggg ccctcctcct
     5641 ggtttgctca ctcctcctgt ggccacagaa ctgtcccaga atgccaggca ccttcagggt
     5701 ggggagaaac agcgggtctt cactggtatt gttaccagct tgcatgacta ctttggggtt
     5761 gtggatgaag aggtcttttt tcagctaagt gtggtgaagg gccgtctgcc ccagctgggt
     5821 gagaaggtgc tggtgaaggc tgcatacaac ccaggccagg cagtgccctg gaatgctgtc
     5881 aaggtgcaaa cgctctccaa ccagccccta ctgaagtccc cagcacctcc tcttctgcat
     5941 gtagcagccc tgggccagaa gcaagggatc ctgggagctc agcctcagtt gatcttccag
     6001 cctcaccgga ttcccccact ctttcctcag aagcctctga gtctcttcca aacatcccac
     6061 acacttcacc tgagccacct gaacagattt cctgcccggg gccctcatgg acggttggat
     6121 cagggccgaa gtgatgacta tgactccaag aaacgcaaac agcgggctgg tggagagccc
     6181 tggggtgcta agaagccaag gcatgacctg cctccttacc gggtccacct cactccttac
     6241 actgtggaca gccccatctg tgacttccta gaactccagc gccgttaccg cagcctcctg
     6301 gtcccctcag attttctgtc cgtgcatctg agttggctat cagccttccc cctgagccag
     6361 cccttttccc tccatcatcc aagccggatc caggtctctt ctgaaaagga ggcagctcca
     6421 gacgctggtg ctgagcccat cactgcagac agtgaccccg cttatagttc gaaggtactg
     6481 ctgctctctt ccccggggtt ggaggaattg tatcgttgtt gcatgctctt tgtggatgac
     6541 atggctgagc caagggagac gccagagcat cctctgaagc agattaagtt tttgctgggc
     6601 aggaaagaag aggaggcagt gctggttggg ggtgaatggt ctccttccct ggatggcctc
     6661 gacccccagg ctgacccgca ggtgctggtg cgtaccgcca tccgctgtgc gcaggcccag
     6721 actggcattg atttgagcgg ctgtaccaag tggtggcgct ttgccgagtt tcagtacctg
     6781 cagccgggac ccccccggcg gcttcagaca gtggtggtgt acctgccgga tgtctggacc
     6841 atcatgccta ctttggagga gtgggaggcc ctgtgccagc agaaagctgc agaggcagct
     6901 cccccaaccc aggaggcaca aggggaaacg gagcctactg aacaggcacc tgatgccttg
     6961 gagcaagcag cagacacttc tagacggaac gcagaaactc cagaggccac cacacagcag
     7021 gaaacggaca ctgatctccc agaggcccct ccaccccccc tagaacctgc tgtcatcgca
     7081 cgccctggct gtgtaaacct gtccctccat gggattgtgg aggatcggag gccaaaggaa
     7141 aggatctctt ttgaggtgat ggtgctggcc gagctgtttc tggagatgct ccagagggat
     7201 tttggctata gagtttataa gatgctactg agccttcctg aaaaggtcgt gtccccacct
     7261 gaacctgaga aggaggaggc ggccaaggaa gaagccacca aggaggaaga agccatcaaa
     7321 gaggaggtgg tcaaggagcc caaggatgag gcacagaatg agggcccggc tacagagtca
     7381 gaggccccgc tgaaggagga tgggcttttg cccaaaccac tctcttctgg gggagaggaa
     7441 gaagaaaaac cccggggcga ggcttctgag gacctgtgtg agatggccct ggacccagaa
     7501 ctgttgcttc tgagggatga tggagaggag gagtttgcag gagcaaagct ggaggattcg
     7561 gaggtccggt ccgttgcctc aaaccagtca gagatggagt tctcttcact tcaggacatg
     7621 cccaaggagc tggatccctc tgctgtgctc cccttagact gtctgcttgc ttttgtgttc
     7681 tttgatgcca actggtgtgg ctacttgcac cggcgagact tagagaggat cctccttacc
     7741 cttgggatcc ggctcagtgc agagcaggcc aagcagctgg tcagcagggt ggtgacccag
     7801 aacatctgcc agtaccggag ccttcagtac agccgccagg agggcctgga tggtggcctt
     7861 cccgaggagg tgctcttcgg aaacctggac ctgctgcccc ctcctgggaa aagcacgaag
     7921 ccaggtgctg cccccacaga acacaaagcc ttggtgtccc acaatggcag cctgattaac
     7981 gtggggagcc tgctgcagcg cgcggagcag caggacagcg gccggctcta cctagagaac
     8041 aagatccaca cactggagct gaagctggag gagagccata accgtttctc agccactgaa
     8101 gtaaccaata agacgctggc ggcagagatg caggagctgc gagtccggct ggcggaggcc
     8161 gaggagaccg cccggacggc ggagcgacag aagagccagc tccagcggct gctgcaggag
     8221 ctccgcaggc gtctgacccc cctgcagctg gagatccagc gggtggtgga aaaggctgac
     8281 agctgggtgg agaaggagga gccggcacct agcaactgag cggccgcact cgagatatct
     8341 agacccagct ttc
//
