LOCUS       sequence-436465-        4258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of a chimeric LacI:PtxS transcription 
            repressor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   O'Neil P
  TITLE     Expanding the Toolbox of LacI/GalR Family Chimeras for 
            Transcriptional Regulation
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LLhPtxS-pHG165a
FEATURES             Location/Qualifiers
     source          1..4258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      194..203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1606..1797
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1791..1813)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1899..2039
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1954..1973
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2225..2813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2305..2324)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2984..3775,3776..3844))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3607..3626
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3845..3949)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4017..4035
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     4188..4210
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4202..4219
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4203..4219
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
       61 attcagggtg gtgaatatga aaccggtgac tttatatgac gttgccgaat atgccggtgt
      121 gagctaccag accgtgagcc gtgttgtgaa tcaagcaagc cacgtgagcg ccaagacccg
      181 tgaaaaagtg gaggccgcca tggccgagct gaattacatt cctaaccgcg tggcacagca
      241 gctggctggt aaacagagtc gtttaatcgg catgctggtg gccgatattc tgaatccgta
      301 tagcgtggcc gtgatgcatg gtgtggaaac cgcttgtcgc cagcatggct atagtctggt
      361 ggtgtgcaat accaaccgcg acgatgaaca agagcgccac catttagtgg cactgcagag
      421 ctacaacgtg gagggtttaa ttgttaacac actgggccat catcccggtg agctgctgaa
      481 tctgcagcgt gatattccga tggtgctggt ggaccgccag ctgcccgaac tgaacgtgga
      541 tctggtgggt ttagataatg ccgatgccgt ggaacaagct ctggatcatc tgcaagctca
      601 aggttatcgc gatattctgg cagtgagcga gccgttagat ggcaccagca gccgtctgga
      661 gcgtgtgcaa gcttttggtg ccagcattag ccgtcgtccc ggtatgcgcc agcaagttct
      721 ggaaatcggt gccggtttac aaggtcagct ggcaagcttt ctggcccata gtggtcacgg
      781 tccgcaagct atttttacct ttaacggcgt tgccacttta gcagttacac gtgcactgct
      841 ggaggccggt cgcaatttag ttgcagacgt tggtctgatc gctttagatg atctggactg
      901 gtatccgctg gtgggcaaag gtattacagc tttagcccag ccgaccgaac gtattggtgt
      961 ggccgccttt gaaagtctgc tgggtcgctt acgcggcgat agtggtgccg cacgccgcat
     1021 cgattttaaa gccaatctga ttattcgtgg tagtacacag cctcagtgat gagaattcgt
     1081 aataatatag ctctagatta attgcgcccg ttaacggcgg gcagcgttgg gtcctggcca
     1141 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
     1201 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
     1261 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
     1321 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
     1381 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
     1441 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
     1501 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
     1561 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
     1621 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
     1681 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
     1741 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     1801 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     1861 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     1921 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     1981 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     2041 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2101 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2161 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2221 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2281 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2341 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2401 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2461 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2521 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2581 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2641 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     2701 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2761 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2821 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2881 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2941 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3001 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3061 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3121 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3181 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3241 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3301 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3361 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3421 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3481 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3541 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3601 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3661 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3721 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3781 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3841 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3901 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3961 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     4021 ggcgtatcac gaggcccttt cgtcttcaag aatccgttaa cggcattcgc cattcaggct
     4081 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     4141 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     4201 ttgtaaaacg acggccagtg ccaagcttgg ctgcaggtcg acggatcccc gggaattc
//
