LOCUS       sequence-43647-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA2k-RFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        187..242
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(334..353)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ttggtaactt tcagtttagc ggtctgggta ccttcgtacg gacgaccttc accttcacct
       61 tcgatttcga actcgtgacc gttaacggaa ccttccatac gaactttgaa acgcatgaac
      121 tctttgataa cgtcttcgct actcgccata tgtatatctc cttcttaaaa gatcttttga
      181 attcttttct ctatcactga tagggagtgg taaaataact ctatcaacga tagagtgtca
      241 acaaaaatta ggaattaatg atgtctagat tagataaaag taaagtgatt aacagcgcat
      301 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
      361 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
      421 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
      481 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
      541 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
      601 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
      661 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
      721 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
      781 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
      841 tatgcggatt agaaaaacaa
//
