LOCUS       sequence-436476-       12672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP tagged human ATG2A with the mutation in LC3 
            interacting region..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12672)
  AUTHORS   Shimizu T, Tamura N, Nishimura T, Saito C, Yamamoto H, Mizushima N
  TITLE     Comprehensive analysis of autophagic functions of WIPI family 
            proteins and their implications for the pathogenesis of 
            beta-propeller associated neurodegeneration.
  JOURNAL   Hum Mol Genet. 2023 Aug 7;32(16):2623-2637. doi: 
            10.1093/hmg/ddad096.
  PUBMED    37364041
REFERENCE   2  (bases 1 to 12672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Hum Mol Genet"; date: "2023-08-7- 7"; volume: "32"; issue: "16"; 
            pages: "2623-2637"
COMMENT     Sequence Label: pMRX-IP-muGFP-hATG2A mLIR
FEATURES             Location/Qualifiers
     source          1..12672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    739..1096
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1161..1577
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1507..1529
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1545..1561
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1587..1961
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1899..1915
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1988..1997
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1994..1996,1997..1999,2000..2710)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVLCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     AYFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EDVTAAGITHGMDELYK"
     primer_bind     complement(2039..2060)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    8583..9149
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8599..9151
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8743..8760)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8970..8989
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9169..9768
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9169..9188)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9665..9685
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9998..10467
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(10597..10614)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10768..11356)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10848..10867)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11520..12311,12312..12380))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12143..12162
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12381..12485)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12553..12571
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg ctagagaacc atcagatgtt tccagggtgc cccaaggacc
      421 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
      481 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
      541 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
      601 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
      661 gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc
      721 gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt
      781 gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc tctgtatctg
      841 gcggacccgt ggtggaactg acgagttcgg aacacccggc cgcaaccctg ggagacgtcc
      901 cagggacttc gggggccgtt tttgtggccc gacctgagtc caaaaatccc gatcgttttg
      961 gactctttgg tgcacccccc ttagaggagg gatatgtggt tctggtagga gacgagaacc
     1021 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttgggaccga agccgcgccg
     1081 cgcgtcttgt ctgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt
     1141 tgtctgaaaa tatgggcccg ggccagactg ttaccactcc cttaagtttg accttaggtc
     1201 actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt
     1261 gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca
     1321 cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg
     1381 gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc
     1441 cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt
     1501 ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca
     1561 ctccttctct aggcgccccc atatggccat atgagatctt atatggggca cccccgcccc
     1621 ttgtaaactt ccctgaccct gacatgacaa gagttactaa cagcccctct ctccaagctc
     1681 acttacaggc tctctactta gtccagcacg aagtctggag acctctggcg gcagcctacc
     1741 aagaacaact ggaccgaccg gtggtacctc acccttaccg agtcggcgac acagtgtggg
     1801 tccgccgaca ccagactaag aacctagaac ctcgctggaa aggaccttac acagtcctgc
     1861 tgaccacccc caccgccctc aaagtagacg gcatcgcagc ttggatacac gccgcccacg
     1921 tgaaggctgc cgaccccggg ggtggaccat cctctagact gccggatctg ccatcgatat
     1981 aaagcttgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2041 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgagaggcg agggcgaggg
     2101 cgatgccacc aatggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2161 gccctggccc accctcgtga ccaccctgac ctacggcgtg ttgtgcttca gccgctaccc
     2221 cgaccacatg aagagacacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2281 gcgcaccatc tcattcaagg acgacggcac atacaagacc cgcgccgagg tgaagttcga
     2341 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2401 catcctgggg cacaagctgg agtacaactt taacagccac aacgtctata tcacagccga
     2461 caagcagaag aacggcatca aggcttattt caagatccgc cacaacgttg aggacggcag
     2521 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2581 gcccgacaac cactacctga gcacccagtc cgttctgagc aaagacccca acgagaagcg
     2641 cgatcacatg gtcctgctgg aggatgtgac cgccgccggg atcactcatg gcatggacga
     2701 gctgtacaag ggttcaggct cgggatccat gtcacgatgg ctgtggccat ggtcaaactg
     2761 tgtgaaagag cgggtctgcc gctacttgct gcaccactac ttaggtcact tcttccaaga
     2821 gcacctcagc ctggaccagc tcagcctcga tctgtacaag ggcagcgttg ccctgcgaga
     2881 catccacctg gaaatctggt ctgtgaacga ggtgctggag tcaatggagt caccgctgga
     2941 gctggtggaa ggcttcgtgg gctccatcga ggtggccgtg ccctgggctg ctctgctcac
     3001 cgaccactgc acagtgcgcg tgtccggcct ccagctcacc ttgcagcccc gccggggtcc
     3061 agcgccaggg gctgccgact cacagagctg ggcctcatgc atgaccacaa gcctgcagct
     3121 ggcccaggag tgtctgcggg atgggctacc ggagccctct gagccaccac agcccctgga
     3181 ggggctggag atgtttgccc agaccattga gactgtgctt cggaggatca aagtgacctt
     3241 cctggacact gtcgtgaggg tggagcactc tccgggtgat ggggaacgtg gtgtggccgt
     3301 cgaggtccgt gtgcagagac tggagtactg tgatgaggca gtgcgggacc caagccaggc
     3361 gccgccggtg gatgtgcatc agccgcctgc cttcctgcac aagctgctgc agctggcagg
     3421 ggtccgcctg cactacgagg agctcccggc acaggaagag cctccagagc cccccttgca
     3481 gatcggcagc tgctcagggt acatggagct gatggtgaag ttgaagcaaa atgaggcctt
     3541 ccctggcccc aagttggagg tggcgggaca gctgggctcc ctgcacctgc tcctgacccc
     3601 gaggcagctc cagcaacttc aggaactgct cagcgccgtg agccttacag accacgaggg
     3661 cctggctgac aagctgaaca agagccgccc gctaggtgcc gaagacctgt ggctgattga
     3721 gcaggacctg aaccagcagc tgcaggcagg ggcagtggct gagcccctca gcccagaccc
     3781 ccttaccaac ccccttctca acctggataa cactgacctc ttcttctcca tggctggcct
     3841 cacaagcagt gtggcctcag ccctctctga gctctccctc tccgatgtag acctggcctc
     3901 ctctgtgcgc agcgacatgg cctcccgccg gctctctgcc caggcccacc cagctggcaa
     3961 gatggccccc aaccccctcc tggacaccat gcgccctgac tcgctgctga agatgacctt
     4021 ggggggtgtg accctgacct tgcttcagac gtctgcccca tcttccggac cacctgacct
     4081 cgccacgcac tttttcaccg agtttgatgc caccaaggat gggcccttcg gttcccgaga
     4141 cttccatcac cttcgaccac gcttccagag ggcctgtccc tgtagccatg ttcggctaac
     4201 gggcacagcc gtgcagctgt cctgggagct gcggacgggc agtcggggcc ggcggacaac
     4261 cagcatggaa gtgcacttcg ggcagctgga ggtgctggag tgtctgtggc cccggggcac
     4321 ctctgagcct gagtacacgg agatcctgac ctttcctggt acgctgggct cccaggcctc
     4381 agctcggccc tgcgcccatc tgcgccacac acagatcctg cgccgtgtgc ctaagagccg
     4441 accccggcgc tcagttgcct gccattgcca ctcagaactg gccctggacc tggccaactt
     4501 ccaggcggac gtggagctgg gggccctgga ccggctggcc gccctactgc gcctggccac
     4561 cgtacctgct gagcctccag ccggcctgct gacagagccc ctgccggcga tggagcagca
     4621 gacggtattt cggctctctg caccccgggc cacgctgcgg ctgcgcttcc ccattgccga
     4681 cctgcggcct gagcgggacc cctgggcggg ccaggccgtg cgggctgagc agcttcggct
     4741 ggagctgagt gagccccagt tccggtcaga gcttagcagt gggcctggtc ccccagtccc
     4801 cacccacctg gaactcacct gctccgacct acatggtatc tatgaagatg gagggaagcc
     4861 acctgtccct tgcctgcgtg tctccaaagc cctggacccc aagagcactg ggcgcaagta
     4921 cttcctgccc caggtagtgg tgactgtgaa cccccagtcc agcagcacac agtgggaggt
     4981 ggccccggag aagggagagg aactggagct gtcagtggag agtccctgtg agctgcggga
     5041 acctgagccc tcgcccttct cctctaagag gaccatgtat gagacagagg agatggtgat
     5101 ccctggagac cctgaggaga tgaggacgtt ccagagccgg accctggcac tgtcccgctg
     5161 cagcctggaa gtgatcctgc ccagtgtcca catctttctg cccagcaagg aggtctacga
     5221 gagcatctac aacaggatca acaacgacct gctcatgtgg gagcctgcag atctgcttcc
     5281 cacccccgac cccgccgccc agccctcggg cttccccggc ccctcaggct tctggcacga
     5341 cagctttaag atgtgcaagt cagccttcaa gctggccaac tgctttgatc tcaccccaga
     5401 ctcggactcg gatgacgagg atgcccactt cttctcagtg ggggcatcag gtggcccaca
     5461 ggccgctgcc cctgaggccc caagtcttca cttgcagagc accttctcta cactggtgac
     5521 agtgctgaag gggcggatca cagccctctg tgagaccaag gatgagggtg ggaagcggct
     5581 ggaggctgtg cacggggagc tggtgctgga catggagcac ggtaccctct tcagcgtctc
     5641 ccagtactgt ggccagccag gacttggcta cttctgtctg gaagctgaaa aggcaacact
     5701 ctaccaccga gcggccgtgg atgactaccc gctgcccagt cacctggacc ttcccagttt
     5761 cgctcccccg gctcagctgg ccccaaccat ctacccatcg gaggaagggg tgaccgagcg
     5821 gggagcctcg ggccgcaagg gccagggccg gggaccccac atgttgtcca ctgctgtgcg
     5881 catccacctg gacccccaca agaatgtgaa ggagttcctg gtgacactgc ggttgcacaa
     5941 agccaccttg cgccactaca tggccctgcc cgagcagagc tggcattccc agttgttgga
     6001 gttcctagac gtgctggatg accctgtgct gggctacctg cccccgacgg tcatcaccat
     6061 cctgcacaca cacctgttct cctgctctgt ggactatagg ccactctacc tcccagtgcg
     6121 tgtcctcatc accgcggaga ccttcactct ctccagcaac atcatcatgg acacctccac
     6181 cttcctgctc aggttcatcc tcgatgactc cgccttgtac ctgtccgaca agtgtgaggt
     6241 ggagaccctg gacctgcggc gagattatgt ctgtgttttg gatgttgacc tcttggaact
     6301 tgtgattaaa acctggaaag ggagcaccga gggcaaactg agccagccac tattcgagct
     6361 gcgctgctcc aacaatgtgg tacacgtgca cagctgtgcc gactcctgtg ccctgctggt
     6421 caacctgctc cagtacgtaa tgagcacagg cgatctgcac cccccacccc ggccccccag
     6481 ccccacggag atcgccggcc agaagctctc ggagagtcct gcctctctgc cctcgtgccc
     6541 cccagtggag acggccctca tcaaccagcg tgacctggcc gacgccctcc tggacaccga
     6601 gcgcagccta cgggagctgg cccagccttc aggtggccac ctccctcagg cgtcgcccat
     6661 ctccgtctac ctattcccag gtgaacggag tggggcccca cccccttcac cacctgtcgg
     6721 gggccctgct ggcagcttag ggtcatgctc agaggagaag gaagatgaaa gggaagagga
     6781 gggcgatgga gacaccctgg acagtgatga ggccgccgcc gccgatgctc ccggcctggg
     6841 catcccgccc cgagatgggg agcctgtggt gacacagctg catcccggcc ccatcgttgt
     6901 gagggacggt tacttctcac ggccgatcgg cagcacggac ttgctgcggg cacctgccca
     6961 tttcccagtg cccagcactc gggtggtgct acgtgaggtc tccctcgtct ggcacctcta
     7021 tgggggccga gactttggcc cccaccccgg ccacagggca agaactggcc tctcaggtcc
     7081 caggagctcc ccttcccgct gctctggccc caaccggccc cagaactcat ggcgcacgca
     7141 ggggggcagc gggcggcagc accatgtcct catggagatc cagctgagca aggtaagctt
     7201 ccagcacgag gtgtacccag cggagccagc cacaggccct gcggccccca gccaggagct
     7261 ggaggagcga ccgctgtccc gtcaggtgtt catcgtgcag gagctggagg tccgagaccg
     7321 gctcgcctcc tcccagatca acaagttcct gtacctacac acgagtgagc ggatgccgcg
     7381 acgtgcccac tctaacatgc tcaccatcaa agcgctgcat gtggccccca ctaccaacct
     7441 gggtgggcct gagtgctgtc tccgcgtctc gctgatgccc ctgcggctca atgtggacca
     7501 ggatgccctc ttcttcctca aggacttctt cactagtctg gtggccggca tcaaccccgt
     7561 ggtcccaggg gagacctccg ctgaggctcg ccccgagact cgagcccagc ccagcagccc
     7621 cctggaaggg caggccgaag gcgtagagac cactggttcg caggaggccc caggaggtgg
     7681 acacagcccc tcccctcctg accagcagcc catctacttc agagagttcc gcttcacgtc
     7741 tgaggtcccc atctggctgg attaccatgg caagcacgtc acgatggacc aggtgggcac
     7801 ttttgctggc ctcctcatcg gcctggccca actcaactgc tccgagctga agctaaagcg
     7861 gctctgttgc aggcacgggc tcctgggtgt ggacaaggtg ctgggctatg ccctcaacga
     7921 gtggctgcag gacatccgca agaaccagct gcccggcctg ctgggaggcg tgggccccat
     7981 gcactcggtt gtccagctct tccaagggtt ccgggacctg ctgtggctgc ccattgagca
     8041 gtacaggaag gatggccgcc tcatgcgggg gctgcagcga ggggctgcct cctttggctc
     8101 atccacagcc tctgccgccc tggaactcag caaccggttg gtacaggcta tccaggccac
     8161 agctgagacc gtgtatgaca tcctgtcccc ggcagccccc gtctcccgct ccctgcagga
     8221 taagcgctct gcgcggaggc tgcgcagggg ccagcagcct gccgacctgc gggagggtgt
     8281 ggccaaggcc tacgacacag tgcgagaggg catcttggat acagctcaga ccatctgtga
     8341 cgtggcatcg cggggccatg agcagaaggg gctgacgggc gccgtggggg gcgtgatccg
     8401 ccagctgccc ccgactgtgg tgaagccgct catcctggcc acggaggcca cgtccagcct
     8461 gctcgggggc atgcgcaacc agattgtccc cgacgcccac aaggaccacg ccctcaagtg
     8521 gcgctcggac agtgcccaag actgagaatt cgggtctcga gtaaagcggc cgctacgtaa
     8581 attccgcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     8641 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     8701 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     8761 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agtttcttga agacaaacaa
     8821 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     8881 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     8941 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     9001 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     9061 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     9121 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccgtcgacat gaccgagtac
     9181 aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc
     9241 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag
     9301 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     9361 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     9421 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     9481 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     9541 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     9601 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     9661 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     9721 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt cgaccgcccg
     9781 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc gacgataaaa
     9841 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     9901 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata
     9961 gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata
    10021 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
    10081 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
    10141 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
    10201 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
    10261 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
    10321 cggcgcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
    10381 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
    10441 tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt
    10501 tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg
    10561 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    10621 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    10681 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    10741 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10801 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10861 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10921 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    10981 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    11041 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    11101 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    11161 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    11221 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    11281 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    11341 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    11401 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    11461 ccttttgcgg ccggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    11521 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11581 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11641 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11701 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    11761 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    11821 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    11881 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    11941 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    12001 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    12061 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    12121 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    12181 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    12241 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12301 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12361 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    12421 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    12481 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    12541 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcactatag gagcttggcc
    12601 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
    12661 taccgccatg tt
//
