LOCUS       sequence-436479-       12576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP tagged human ATG2A which has the mutation in LC3 
            interacting region and lacks WIPI interacting region..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Shimizu T, Tamura N, Nishimura T, Saito C, Yamamoto H, Mizushima N
  TITLE     Comprehensive analysis of autophagic functions of WIPI family 
            proteins and their implications for the pathogenesis of 
            beta-propeller associated neurodegeneration.
  JOURNAL   Hum Mol Genet. 2023 Aug 7;32(16):2623-2637. doi: 
            10.1093/hmg/ddad096.
  PUBMED    37364041
REFERENCE   2  (bases 1 to 12576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Hum Mol Genet"; date: "2023-08-7- 7"; volume: "32"; issue: "16"; 
            pages: "2623-2637"
COMMENT     Sequence Label: pMRX-IP-muGFP-hATG2A mLIR-Delta-WIR
FEATURES             Location/Qualifiers
     source          1..12576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    739..1096
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1161..1577
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1507..1529
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1545..1561
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1587..1961
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1899..1915
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1988..1997
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1994..1996,1997..1999,2000..2710)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVLCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     AYFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EDVTAAGITHGMDELYK"
     primer_bind     complement(2039..2060)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    8487..9053
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8503..9055
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8647..8664)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8874..8893
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9073..9672
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9073..9092)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9569..9589
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9902..10371
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(10501..10518)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10672..11260)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10752..10771)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11424..12215,12216..12284))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12047..12066
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12285..12389)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12457..12475
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg ctagagaacc atcagatgtt tccagggtgc cccaaggacc
      421 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
      481 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
      541 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
      601 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
      661 gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc
      721 gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt
      781 gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc tctgtatctg
      841 gcggacccgt ggtggaactg acgagttcgg aacacccggc cgcaaccctg ggagacgtcc
      901 cagggacttc gggggccgtt tttgtggccc gacctgagtc caaaaatccc gatcgttttg
      961 gactctttgg tgcacccccc ttagaggagg gatatgtggt tctggtagga gacgagaacc
     1021 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttgggaccga agccgcgccg
     1081 cgcgtcttgt ctgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt
     1141 tgtctgaaaa tatgggcccg ggccagactg ttaccactcc cttaagtttg accttaggtc
     1201 actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt
     1261 gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca
     1321 cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg
     1381 gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc
     1441 cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt
     1501 ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca
     1561 ctccttctct aggcgccccc atatggccat atgagatctt atatggggca cccccgcccc
     1621 ttgtaaactt ccctgaccct gacatgacaa gagttactaa cagcccctct ctccaagctc
     1681 acttacaggc tctctactta gtccagcacg aagtctggag acctctggcg gcagcctacc
     1741 aagaacaact ggaccgaccg gtggtacctc acccttaccg agtcggcgac acagtgtggg
     1801 tccgccgaca ccagactaag aacctagaac ctcgctggaa aggaccttac acagtcctgc
     1861 tgaccacccc caccgccctc aaagtagacg gcatcgcagc ttggatacac gccgcccacg
     1921 tgaaggctgc cgaccccggg ggtggaccat cctctagact gccggatctg ccatcgatat
     1981 aaagcttgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2041 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgagaggcg agggcgaggg
     2101 cgatgccacc aatggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2161 gccctggccc accctcgtga ccaccctgac ctacggcgtg ttgtgcttca gccgctaccc
     2221 cgaccacatg aagagacacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2281 gcgcaccatc tcattcaagg acgacggcac atacaagacc cgcgccgagg tgaagttcga
     2341 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2401 catcctgggg cacaagctgg agtacaactt taacagccac aacgtctata tcacagccga
     2461 caagcagaag aacggcatca aggcttattt caagatccgc cacaacgttg aggacggcag
     2521 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2581 gcccgacaac cactacctga gcacccagtc cgttctgagc aaagacccca acgagaagcg
     2641 cgatcacatg gtcctgctgg aggatgtgac cgccgccggg atcactcatg gcatggacga
     2701 gctgtacaag ggttcaggct cgggatcctc acgatggctg tggccatggt caaactgtgt
     2761 gaaagagcgg gtctgccgct acttgctgca ccactactta ggtcacttct tccaagagca
     2821 cctcagcctg gaccagctca gcctcgatct gtacaagggc agcgttgccc tgcgagacat
     2881 ccacctggaa atctggtctg tgaacgaggt gctggagtca atggagtcac cgctggagct
     2941 ggtggaaggc ttcgtgggct ccatcgaggt ggccgtgccc tgggctgctc tgctcaccga
     3001 ccactgcaca gtgcgcgtgt ccggcctcca gctcaccttg cagccccgcc ggggtccagc
     3061 gccaggggct gccgactcac agagctgggc ctcatgcatg accacaagcc tgcagctggc
     3121 ccaggagtgt ctgcgggatg ggctaccgga gccctctgag ccaccacagc ccctggaggg
     3181 gctggagatg tttgcccaga ccattgagac tgtgcttcgg aggatcaaag tgaccttcct
     3241 ggacactgtc gtgagggtgg agcactctcc gggtgatggg gaacgtggtg tggccgtcga
     3301 ggtccgtgtg cagagactgg agtactgtga tgaggcagtg cgggacccaa gccaggcgcc
     3361 gccggtggat gtgcatcagc cgcctgcctt cctgcacaag ctgctgcagc tggcaggggt
     3421 ccgcctgcac tacgaggagc tcccggcaca ggaagagcct ccagagcccc ccttgcagat
     3481 cggcagctgc tcagggtaca tggagctgat ggtgaagttg aagcaaaatg aggccttccc
     3541 tggccccaag ttggaggtgg cgggacagct gggctccctg cacctgctcc tgaccccgag
     3601 gcagctccag caacttcagg aactgctcag cgccgtgagc cttacagacc acgagggcct
     3661 ggctgacaag ctgaacaaga gccgcccgct aggtgccgaa gacctgtggc tgattgagca
     3721 ggacctgaac cagcagctgc aggcaggggc agtggctgag cccctcagcc cagaccccct
     3781 taccaacccc cttctcaacc tggataacac tgacctcttc ttctccatgg ctggcctcac
     3841 aagcagtgtg gcctcagccc tctctgagct ctccctctcc gatgtagacc tggcctcctc
     3901 tgtgcgcagc gacatggcct cccgccggct ctctgcccag gcccacccag ctggcaagat
     3961 ggcccccaac cccctcctgg acaccatgcg ccctgactcg ctgctgaaga tgaccttggg
     4021 gggtgtgacc ctgaccttgc ttcagacgtc tgccccatct tccggaccac ctgacctcgc
     4081 cacgcacttt ttcaccgagt ttgatgccac caaggatggg cccttcggtt cccgagactt
     4141 ccatcacctt cgaccacgct tccagagggc ctgtccctgt agccatgttc ggctaacggg
     4201 cacagccgtg cagctgtcct gggagctgcg gacgggcagt cggggccggc ggacaaccag
     4261 catggaagtg cacttcgggc agctggaggt gctggagtgt ctgtggcccc ggggcacctc
     4321 tgagcctgag tacacggaga tcctgacctt tcctggtacg ctgggctccc aggcctcagc
     4381 tcggccctgc gcccatctgc gccacacaca gatcctgcgc cgtgtgccta agagccgacc
     4441 ccggcgctca gttgcctgcc attgccactc agaactggcc ctggacctgg ccaactttca
     4501 ggcggacgtg gagctggggg ccctggaccg gctggccgcc ctactgcgcc tggccaccgt
     4561 acctgctgag cctccagccg gcctgctgac agagcccctg ccggcgatgg agcagcagac
     4621 ggtatttcgg ctctctgcac cccgggccac gctgcggctg cgcttcccca ttgccgacct
     4681 gcggcctgag cgggacccct gggcgggcca ggccgtgcgg gctgagcagc ttcggctgga
     4741 gctgagtgag ccccagttcc ggtcagagct tagcagtggg cctggtcccc cagtccccac
     4801 ccacctggaa ctcacctgct ccgacctaca tggtatctat gaagatggag ggaagccacc
     4861 tgtcccttgc ctgcgtgtct ccaaagccct ggaccccaag agcactgggc gcaagtactt
     4921 cctgccccag gtagtggtga ctgtgaaccc ccagtccagc agcacacagt gggaggtggc
     4981 cccggagaag ggagaggaac tggagctgtc agtggagagt ccctgtgagc tgcgggaacc
     5041 tgagccctcg cccttctcct ctaagaggac catgtatgag acagaggaga tggtgatccc
     5101 tggagaccct gaggagatga ggacgttcca gagccggacc ctagcactgt cccgctgcag
     5161 cctggaagtg atcctgccca gtgtccacat ctttctgccc agcaaggagg tctacgagag
     5221 catctacaac aggatcaaca acgacctgct catgtgggag cctgcagatc tgcttcccac
     5281 ccccgacccc gccgcccagc cctcgggctt ccccggcccc tcaggcttct ggcacgacag
     5341 ctttaagatg tgcaagtcag ccttcaagct ggccaactgc tttgatctca ccccagactc
     5401 ggactcggat gacgaggatg cccacttctt ctcagtgggg gcatcaggtg gcccacaggc
     5461 cgctgcccct gaggccccaa gtcttcactt gcagagcacc ttctctacac tggtgacagt
     5521 gctgaagggg cggatcacag ccctctgtga gaccaaggat gagggtggga agcggctgga
     5581 ggctgtgcac ggggagctgg tgctggacat ggagcacggt accctcttca gcgtctccca
     5641 gtactgtggc cagccaggac ttggctactt ctgtctggaa gctgaaaagg caacactcta
     5701 ccaccgagcg gccgtggatg actacccgct gcccagtcac ctggaccttc ccagtttcgc
     5761 tcccccggct cagctggccc caaccatcta cccatcggag gaaggggtga ccgagcgggg
     5821 agcctcgggc cgcaagggcc agggccgggg accccacatg ttgtccactg ctgtgcgcat
     5881 ccacctggac ccccacaaga atgtgaagga gttcctggtg acactgcggt tgcacaaagc
     5941 caccttgcgc cactacatgg ccctgcccga gcagagctgg cattcccagt tgttggagtt
     6001 cctagacgtg ctggatgacc ctgtgctggg ctacctgccc ccgacggtca tcaccatcct
     6061 gcacacacac ctgttctcct gctctgtgga ctataggcca ctctacctcc cagtgcgtgt
     6121 cctcatcacc gcggagacct tcactctctc cagcaacatc atcatggaca cctccacctt
     6181 cctgctcagg ttcatcctcg atgactccgc cttgtacctg tccgacaagt gtgaggtgga
     6241 gaccctggac ctgcggcgag attatgtctg tgttttggat gttgacctct tggaacttgt
     6301 gattaaaacc tggaaaggga gcaccgaggg caaactgagc cagccactat tcgagctgcg
     6361 ctgctccaac aatgtggtac acgtgcacag ctgtgccgac tcctgtgccc tgctggtcaa
     6421 cctgctccag tacgtaatga gcacaggcga tctgcacccc ccaccccggc cccccagccc
     6481 cacggagatc gccggccaga agctctcgga gagtcctgcc tctctgccct cgtgcccccc
     6541 agtggagacg gccctcatca accagcgtga cctggccgac gccctcctgg acaccgagcg
     6601 cagcctacgg gagctggccc agccttcagg tggccacctc cctcaggcgt cgcccatctc
     6661 cgtctaccta ttcccaggtg aacggagtgg ggccccaccc ccttcaccac ctgtcggggg
     6721 ccctgctggc agcttagggt catgctcaga ggagaaggaa gatgaaaggg aagaggaggg
     6781 cgatggagac accctggaca gtgatgaggc cgccgccgcc gatgctcccg gcctgggcat
     6841 cccgttgctg cgggcacctg cccatttccc agtgcccagc actcgggtgg tgctacgtga
     6901 ggtctccctc gtctggcacc tctatggggg ccgagacttt ggcccccacc ccggccacag
     6961 ggcaagaact ggcctctcag gtcccaggag ctccccttcc cgctgctctg gccccaaccg
     7021 gccccagaac tcatggcgca cgcagggggg cagcgggcgg cagcaccatg tcctcatgga
     7081 gatccagctg agcaaggtaa gcttccagca cgaggtgtac ccagcggagc cagccacagg
     7141 ccctgcggcc cccagccagg agctggagga gcgaccgctg tcccgtcagg tgttcatcgt
     7201 gcaggagctg gaggtccgag accggctcgc ctcctcccag atcaacaagt tcctgtacct
     7261 acacacgagt gagcggatgc cgcgacgtgc ccactctaac atgctcacca tcaaagcgct
     7321 gcatgtggcc cccactacca acctgggtgg gcctgagtgc tgtctccgcg tctcgctgat
     7381 gcccctgcgg ctcaatgtgg accaggatgc cctcttcttc ctcaaggact tcttcactag
     7441 tctggtggcc ggcatcaacc ccgtggtccc aggggagacc tccgctgagg ctcgccccga
     7501 gactcgagcc cagcccagca gccccctgga agggcaggcc gaaggcgtag agaccactgg
     7561 ttcgcaggag gccccaggag gtggacacag cccctcccct cctgaccagc agcccatcta
     7621 cttcagagag ttccgcttca cgtctgaggt ccccatctgg ctggattacc atggcaagca
     7681 cgtcacgatg gaccaggtgg gcacttttgc tggcctcctc atcggcctgg cccaactcaa
     7741 ctgctccgag ctgaagctaa agcggctctg ttgcaggcac gggctcctgg gtgtggacaa
     7801 ggtgctgggc tatgccctca acgagtggct gcaggacatc cgcaagaacc agctgcccgg
     7861 cctgctggga ggcgtgggcc ccatgcactc ggttgtccag ctcttccaag ggttccggga
     7921 cctgctgtgg ctgcccattg agcagtacag gaaggatggc cgcctcatgc gggggctgca
     7981 gcgaggggct gcctcctttg gctcatccac agcctctgcc gccctggaac tcagcaaccg
     8041 gttggtacag gctatccagg ccacagctga gaccgtgtat gacatcctgt ccccggcagc
     8101 ccccgtctcc cgctccctgc aggataagcg ctctgcgcgg aggctgcgca ggggccagca
     8161 gcctgccgac ctgcgggagg gtgtggccaa ggcctacgac acagtgcgag agggcatctt
     8221 ggatacagct cagaccatct gtgacgtggc atcgcggggc catgagcaga aggggctgac
     8281 gggcgccgtg gggggcgtga tccgccagct gcccccgact gtggtgaagc cgctcatcct
     8341 ggccacggag gccacgtcca gcctgctcgg gggcatgcgc aaccagattg tccccgacgc
     8401 ccacaaggac cacgccctca agtggcgctc ggacagtgcc caagactgag aattcgggtc
     8461 tcgagtaaag cggccgctac gtaaattccg cccccccccc taacgttact ggccgaagcc
     8521 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     8581 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     8641 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     8701 tggaagtttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     8761 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     8821 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     8881 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     8941 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     9001 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     9061 acaaccgtcg acatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     9121 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     9181 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     9241 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     9301 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     9361 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     9421 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     9481 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     9541 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     9601 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     9661 cccggtgcct gagtcgaccg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     9721 cgaccccatg gctcgacgat aaaataaaag attttattta gtctccagaa aaagggggga
     9781 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     9841 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca
     9901 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     9961 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
    10021 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
    10081 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
    10141 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
    10201 taaaagagcc cacaacccct cactcggcgc gccagtcctc cgattgactg agtcgcccgg
    10261 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
    10321 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc acatgcagca
    10381 tgtatcaaaa ttaatttggt tttttttctt aagtatttac attaaatggc catagttgca
    10441 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    10501 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10561 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10621 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10681 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10741 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10801 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10861 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10921 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10981 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    11041 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    11101 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    11161 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    11221 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    11281 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    11341 atcaaaaagg atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata
    11401 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    11461 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    11521 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    11581 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    11641 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    11701 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    11761 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    11821 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    11881 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    11941 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    12001 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
    12061 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    12121 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    12181 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    12241 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    12301 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    12361 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    12421 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    12481 cgtcttcact ataggagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
    12541 tttatattgg ctcatgtcca acattaccgc catgtt
//
