LOCUS       sequence-436485-        5257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a 
            modification of the SF3B3-miniXon system. Translation of Firefly 
            luciferase is controlled by splicing modulation of the SF3B3-XS3B by
            L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5257)
  TITLE     Plasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a 
            modification of the SF3B3-miniXon system. Translation of Firefly 
            luciferase is controlled by splicing modulation of the SF3B3-XS3B by
            LMI070.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGL4.10/RSV_XS3B-miniXon_Luciferase
FEATURES             Location/Qualifiers
     source          1..5257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      818..827
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      994..1003
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1351..1613)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    1842..1933
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(1874..1893)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    1947..1995
                     /note="synthetic polyadenylation signal"
     CDS             join(2100..2168,2169..2960)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3160..3748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3649..3668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3902..3919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    4171..4292
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4208..4227)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4262..4281
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(4336..5257,1..728))
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 tgaaatggca ccacgctgag gatagcggtg tcggggatga tctggttgcc gaagatgggg
       61 tcgcgggcat gactgaatcg gacacaagcg gtgcggtgcg gtagggctac gcccttgggc
      121 aatccggtac tgccactact gttcatgatc agggcgatgg ttttgtcccg gtcgaagctc
      181 tcgggcacga agtcgtactc gttgaagccg ggtggcaaat gggaagtcac gaaggtgtac
      241 atgctttgga agccctggta gtcggtcttg ctatccatga tgatgatctt ttgtatgatc
      301 ggtagcttct tttgcacgtt gaggatcttt tgcagccctt tcttgctcac gaatacgacg
      361 gtgggctggc tgatgcccat gctgttcagc agctcgcgct cgttgtagat gtcgttagct
      421 ggggccacag ccacaccgat gaacagggca cccaacacgg gcatgaagaa ctgcaagcta
      481 ttctcgctgc acaccacgat ccgatggttt gtattcagcc catagcgctt catagcttct
      541 gccagccgaa cgctcatctc gaagtactcg gcgtaggtaa tgtccacctc gatatgtgcg
      601 tcggtaaagg cgatggtgcc gggcaccagg gcgtagcgct tcatggcttt gtgcagctgc
      661 tcgccggcgg tcccgtcttc gagtgggtag aatggcgctg ggcccttctt aatgtttttg
      721 gcatcttcgg atccgaccca cgggaaacaa caattttttg ttgtttggtt cctagatcac
      781 caaaaaataa agatcataaa caaccagaac cagcccggcc accatggtga aaccccgttt
      841 ttattaaaaa tacaaaaatt agccaggcgt ggaatgttgt gtggtcagtt aggctgcttc
      901 tcagtaccac attaagtatt cagtaccaca ttaagtattc agagttaaat ttcaacaaaa
      961 ttatgtctta ctctacacaa tcatgaactt tttccatggt ggcacttgga ttgactcaaa
     1021 aatgttaatc tgcaaatggc aagaaaaagt ttttatttta taaacattag tacctcactt
     1081 tcagtaactt tgtatttcct aatccaatat ttgaaatttc acacaatttt tgttttgtca
     1141 taaatggaaa acattttttg cacaagagat taagcaagat atgtattaat tcacttaaaa
     1201 tttcaaaaga tggtaacgag agaacttacc agaaaagtta ccaagaaagg caaagctgaa
     1261 gccagtggct ctctgtaagg ttaagttgta gagaaattta ccaacagtac cggattgcca
     1321 agcttgttta aacggatctg tggccttgga ggtgcacacc aatgtggtga atggtcaaat
     1381 ggcgtttatt gtatcgagct aggcacttaa atacaattat ctctgcaatg cgcaattcag
     1441 tggttcgtcc aatccatgtc agacctgtct gttgccttcc taataaggca cgatcgtacc
     1501 accttacttc caccaatcgg catgcacggt gctttttctc tccttgtaag gcatgttgct
     1561 aactcatcgt taccatgttg caagactaca agtgtattgc ataagactac atttccccct
     1621 ccctatgcaa aagcgaaact actatatcct gaggggactc ctaaccgcgt acaaccgaag
     1681 ccccgctttt cgcctaaaca caccctagtc ccctcagata cgcgtatatc tggcccgtac
     1741 atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcgcctgc
     1801 aggctcgagg ctagcgagct caggtaccgg ccagttaggc cagagaaatg ttctggcacc
     1861 tgcacttgca ctggggacag cctattttgc tagtttgttt tgtttcgttt tgttttgatg
     1921 gagagcgtat gttagtacta tcgattcaca caaaaaacca acacacagat gtaatgaaaa
     1981 taaagatatt ttattgcggc ctgtccaata cctcccgtac cttaatatta cttacttatc
     2041 cttgagagac gtactagtaa ccctgataaa tgcttcaata atattgaaaa aggacgagta
     2101 tgagcatcca acattttcgt gtcgcactca ttcccttctt tgcggcattt tgcttgcctg
     2161 tttttgcaca ccccgaaacg ctggtgaaag taaaagatgc tgaagatcaa ctgggtgcaa
     2221 gagtgggcta tatcgaactg gatctcaata gcggcaagat ccttgagtct ttccgccccg
     2281 aagaacgatt cccgatgatg agcactttca aagtactgct atgtggcgcg gtgttgtccc
     2341 gtatagacgc cgggcaagag cagcttggtc gccgtataca ctactcacaa aacgacttgg
     2401 ttgagtactc gccggtcacg gaaaagcatc ttacggatgg catgacggta agagaattgt
     2461 gtagtgctgc cattaccatg agcgacaaca ccgcggccaa cttacttctg acaacgatcg
     2521 gaggccctaa ggagctgact gcatttcttc ataatatggg tgatcatgtg acccggcttg
     2581 accgctggga accagagttg aacgaagcca taccgaacga cgagcgtgat accacgatgc
     2641 cagtagcaat ggccacaact cttcggaaac tactcactgg cgaacttctt actctagcat
     2701 cacgacagca gctcatagac tggatggagg cggacaaagt agcaggacca cttcttcgct
     2761 cggccctccc tgctggctgg ttcattgctg acaaatcggg ggccggtgaa cgcggctctc
     2821 gcggcatcat tgctgcgctg gggcctgatg gtaagccctc acgaatcgta gtgatctaca
     2881 cgacggggag tcaggccact atggacgaac gaaataggca gatcgctgag atcggtgcct
     2941 cactgatcaa gcactggtaa ccactgcagt ggtttagcat ttgcggccgc tgtcagacca
     3001 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3061 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3121 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3181 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3241 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3301 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3361 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3421 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3481 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3541 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3601 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3661 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3721 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3781 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3841 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3901 cagcgagtca gtgagcgagg aagcggaaga gcgctgccgg cacctgtcct acgagttgca
     3961 tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg
     4021 agctgactgg gttgaaggct ctcaagggca tcggtcgacg gatcgatcct tatcgatttt
     4081 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     4141 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     4201 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4261 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gctcgaagcg gccggccgcc
     4321 ccgactctag aattattaca cggcgatctt gccgcccttc ttggccttaa tgagaatctc
     4381 gcggatcttg cgggcgtcca acttgccggt cagtccttta ggcacctcgt ccacgaacac
     4441 aacaccaccg cgcagcttct tggcggttgt aacctggctg gccacatagt ccacgatctc
     4501 cttctcggtc atggttttac cgtgttccag cacgacgact gcggcgggca gctcgccggc
     4561 atcgtcgtcg ggcaggccgg cgaccccggc gtcgaagatg ttggggtgtt gcagcaggat
     4621 gctctccagt tcggctgggg ctacctggta gcccttgtat ttgatcaggc tcttcagccg
     4681 gtccacgatg aagaagtgct cgtcctcgtc ccagtaggcg atgtcgccgc tgtgcagcca
     4741 gccgtccttg tcgatgagag cgtttgtagc ctcggggttg ttaacgtagc cgctcatgat
     4801 catggggcca cggacgcaca gctcgccgcg ctggttcaca cccagtgtct taccggtgtc
     4861 caagtccacc accttagcct cgaagaaggg caccaccttg cctactgcgc caggcttgtc
     4921 gtccccttcg ggggtgatca gaatggcgct ggttgtttct gtcaggccgt agccctggcg
     4981 gatgcctggt aggtggaagc gtttggccac ggcctcacct acctccttgc tgagcggcgc
     5041 cccgccgctg gcgatctcgt gcaagttgct taggtcgtac ttgtcgatga gagtgctctt
     5101 agcgaagaag ctaaatagtg tgggcaccag cagggcagat tgaatcttat agtcttgcaa
     5161 gctgcgcaag aatagctcct cctcgaagcg gtacatgagc acgacccgaa agccgcagat
     5221 caagtagccc agcgtggtga acatgccgaa gccgtgg
//
