LOCUS       sequence-436491-        6074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for Vesicular Stomatitis Virus Indiana strain 
            nucleoprotein gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6074)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-VSVN
FEATURES             Location/Qualifiers
     source          1..6074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3096..3115)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3161..3216
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3215..3234)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3577..3593)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3577..3593)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3590..3612)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3601..3617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3625..3631,3632..3649,3650..3655))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3670..3691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3750..3945
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3796..3931
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3951..4085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3988..4007)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4042..4061
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4153..4170)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4324..4912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4404..4423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5083..5874,5875..5943))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5706..5725
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5944..6048)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggcgc gccrgggggg gggggggggg gsggggggsg
      541 gggsggggsg rggcggagag gkgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gkgggggtgc cgggcggggc
     1261 ggggccgcck cgggccgggg rgggctcggg ggrggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgccgccac
     1741 catgtctgtt acagtcaaga gaatcattga caacacagtc atagttccaa aacttcctgc
     1801 aaatgaggat ccagtggaat acccggcaga ttacttcaga aaatcaaagg agattcctct
     1861 ttacatcaat actacaaaaa gtttgtcaga tctaagagga tatgtctacc aaggcctcaa
     1921 atccggaaat gtatcaatca tacatgtcaa cagctacttg tatggagcat taaaggacat
     1981 ccggggtaag ttggataaag attggtcaag tttcggaata aacatcggga aagcagggga
     2041 tacaatcgga atatttgacc ttgtatcctt gaaagccctg gacggcgtac ttccagatgg
     2101 agtatcggat gcttccagaa ccagcgcaga tgacaaatgg ttgcctttgt atctacttgg
     2161 cttatacaga gtgggcagaa cacaaatgcc tgaatacaga aaaaagctca tggatgggct
     2221 gacaaatcaa tgcaaaatga tcaatgaaca gtttgaacct cttgtgccag aaggtcgtga
     2281 catttttgat gtgtggggaa atgacagtaa ttacacaaaa attgtcgctg cagtggacat
     2341 gttcttccac atgttcaaaa aacatgaatg tgcctcgttc agatacggaa ctattgtttc
     2401 cagattcaaa gattgtgctg cattggcaac atttggacac ctctgcaaaa taaccggaat
     2461 gtctacagaa gatgtaacga cctggatctt gaaccgagaa gttgcagatg aaatggtcca
     2521 aatgatgctt ccaggccaag aaattgacaa ggccgattca tacatgcctt atttgatcga
     2581 ctttggattg tcttctaagt ctccatattc ttccgtcaaa aaccctgcct tccacttctg
     2641 ggggcaattg acagctcttc tgctcagatc caccagagca aggaatgccc gacagcctga
     2701 tgacattgag tatacatctc ttactacagc aggtttgttg tacgcttatg cagtaggatc
     2761 ctctgccgac ttggcacaac agttttgtgt tggagataac aaatacactc cagatgatag
     2821 taccggagga ttgacgacta atgcaccgcc acaaggcaga gatgtggtcg aatggctcgg
     2881 atggtttgaa gatcaaaaca gaaaaccgac tcctgatatg atgcagtatg cgaaaagagc
     2941 agtcatgtca ctgcaaggcc taagagagaa gacaattggc aagtatgcta agtcagaatt
     3001 tgacaaatga gcggccgcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     3061 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     3121 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     3181 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     3241 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     3301 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     3361 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     3421 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     3481 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     3541 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3601 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3661 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3721 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     3781 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3841 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3901 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
     3961 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4021 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4081 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4141 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4201 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4261 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4321 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4381 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4441 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4501 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4561 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4621 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4681 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4741 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4801 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4861 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4921 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4981 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5041 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5101 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5161 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5221 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5281 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5341 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5401 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5461 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5521 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5581 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5641 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5701 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5761 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5821 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5881 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5941 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6001 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6061 aaaagtgcca cctg
//
