LOCUS       sequence-436541-        8841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of synthetic protein composed of 5 repeats of BRD4 
            bromodomain #1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 8841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pMTTH+bromo5
FEATURES             Location/Qualifiers
     source          1..8841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(181..186,187..202,203..209)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    217..233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     222..244
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             303..1403
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1260..1269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1377..1400
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1452..1463
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1476..1496
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3633..3653
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3654..3671
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3684..3701)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3684..3700)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3693..3715)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3879..3896)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4049..4135
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4227..4254
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4273..4364
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4365..4433,4434..5225)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4583..4602)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5267..5780)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5462..5483)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5674..5693
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5891..6479
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6380..6399
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6633..6650
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6756..6775)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6916..6938
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6932..7123)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        7619..7696
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             7697..8779
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7716..7735)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 atcatcgact gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt
       61 atggctgtgc aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc actcccgttc
      121 tggataatgt tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg
      181 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca
      241 caggaaacag ccagtccgtt taggtgtttt cacgagcact tcaccaacaa ggaccataga
      301 ttatgaaaac tgaagaaggt aaactggtaa tctggattaa cggcgataaa ggctataacg
      361 gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc accgttgagc
      421 atccggataa actggaagag aaattcccac aggttgcggc aactggcgat ggccctgaca
      481 ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg ttggctgaaa
      541 tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat gccgtacgtt
      601 acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg atttataaca
      661 aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg gataaagaac
      721 tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc
      781 cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag tacgacatta
      841 aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg gttgacctga
      901 ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct gcctttaata
      961 aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc gacaccagca
     1021 aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg
     1081 ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg gcaaaagagt
     1141 tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa gacaaaccgc
     1201 tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca cgtattgccg
     1261 ccaccatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt
     1321 tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag actgtcgatg
     1381 aagccctgaa agacgcgcag actaattcga gctcgaacaa caacaacaat aacaataaca
     1441 acaacctcgg gatcgaggga aggatttcag aatttgaaaa cctgtatttt cagggccata
     1501 tggctgcctc gactaatcct ccaccgccgg agaccagtaa cccaaacaag cctaaacgtc
     1561 aaacgaacca actccaatat ctgttgcgtg ttgttttgaa aacattgtgg aaacaccaat
     1621 ttgcatggcc ttttcaacaa ccggttgacg cggtgaagtt aaaccttccg gattattaca
     1681 aaattatcaa aactccgatg gatatgggga ctatcaaaaa acggttggag aacaactact
     1741 actggaatgc ccaagagtgt attcaagatt tcaacaccat gttcacaaac tgttatatct
     1801 acaacaaacc aggagacgat atcgttctga tggccgaggc ccttgagaaa ttatttttac
     1861 agaaaatcaa cgaactcccg acagaggagg gcgggtccgg tggaagtggc ggtagtggtg
     1921 ggagtggtgg atcagccgcc agcacaaacc cacctccgcc tgaaacgtcc aacccgaaca
     1981 aaccgaaacg gcagactaac cagctgcagt acttacttcg ggtagtattg aagacactgt
     2041 ggaagcatca gttcgcctgg ccgttccaac agccagtaga tgcagttaaa cttaatttac
     2101 ctgattatta taaaatcatt aaaacaccta tggatatggg aactattaag aaacgcctgg
     2161 agaataacta ttattggaat gctcaagaat gtatccagga tttcaatacc atgtttacga
     2221 actgttacat ttacaataaa cctggtgatg atatcgtgct tatggcagaa gcactggaaa
     2281 agctgttttt gcaaaaaatt aatgagctgc ctactgagga agggggaagc gggggctcgg
     2341 gggggtcagg cggttctgga ggatctgccg catccactaa cccgcctccg ccagagacga
     2401 gtaatccgaa caagccgaag cgccaaacga atcaattaca gtatcttctt cgtgtcgtgt
     2461 taaaaacgct ttggaagcac cagtttgcgt ggccattcca gcagcctgtg gatgcggtta
     2521 aattaaatct cccggactat tacaagatca tcaaaacccc tatggatatg ggcacgatca
     2581 agaagcgcct cgaaaacaat tactattgga atgcgcaaga atgcatccag gactttaata
     2641 cgatgtttac taactgctat atttataata aaccgggcga tgatattgta ttgatggcgg
     2701 aagctcttga aaaattgttt cttcagaaga tcaatgaact ccctaccgaa gagggaggtt
     2761 cgggagggag cggtggatct ggaggctccg gagggtctgc tgcgtctacc aatccgccac
     2821 caccggaaac tagcaatcct aacaaaccaa aacgtcagac caatcaactg caatacctct
     2881 tacgcgttgt gcttaaaacc ctctggaaac accagtttgc ctggcctttc cagcaaccag
     2941 tggacgccgt gaagttgaac ctgccagact actataagat cattaagacg ccaatggata
     3001 tgggtacaat taagaagcgg ttggaaaaca actattattg gaatgcacag gaatgtatcc
     3061 aagacttcaa tactatgttt acaaattgct acatctataa taagccggga gatgacattg
     3121 ttttaatggc tgaggcatta gaaaagttgt tcttacaaaa gattaatgaa ttgcctacgg
     3181 aagaaggtgg ctctgggggc tcaggtggta gtgggggttc cgggggctcc gcagcctcga
     3241 ccaaccctcc tccacctgag acctctaatc caaataaacc taagcggcaa accaatcagc
     3301 tccagtacct gcttcgcgta gtactgaaga ccttatggaa acatcaattc gcttggccgt
     3361 ttcaacagcc tgttgatgct gttaagctga acttgcctga ttactacaag attatcaaga
     3421 ccccgatgga catggggacg attaaaaagc ggcttgaaaa taattattat tggaacgccc
     3481 aggagtgcat tcaggatttt aacacgatgt tcaccaattg ttatatttac aacaagccag
     3541 gggacgacat cgtactcatg gcagaggcac ttgagaaact tttcctccag aaaattaacg
     3601 agttaccaac cgaggagggc tcgagcggat ccgaaaacct gtattttcag ggccatcatc
     3661 atcatcatca ctgaaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     3721 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     3781 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     3841 cagcttggct gttttggcgg atgagataag attttcagcc tgatacagat taaatcagaa
     3901 cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct
     3961 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
     4021 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
     4081 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     4141 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
     4201 ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt
     4261 tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4321 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4381 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4441 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4501 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgtttcccaa
     4561 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     4621 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4681 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4741 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4801 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4861 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4921 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4981 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5041 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5101 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5161 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5221 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaccc cggttgataa
     5281 tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat atttaaattg taaacgttaa
     5341 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     5401 cgaaatcggc aaaatccctt ataaatcaaa agaatagccc gagatagggt tgagtgttgt
     5461 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     5521 aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttggg
     5581 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     5641 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     5701 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     5761 tgcgccgcta cagggcgcgt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5821 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5881 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5941 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6001 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     6061 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6121 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6181 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6241 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6301 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6361 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6421 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6481 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6541 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6601 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6661 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatgtggt
     6721 gcactctcag tacaccgcat gtggtgcact ctcagtacaa tctgctctga tgccgcatag
     6781 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     6841 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     6901 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     6961 gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg cagcgattca cagatgtctg
     7021 cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga
     7081 taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag
     7141 ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac
     7201 gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg
     7261 tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata
     7321 cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa
     7381 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
     7441 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
     7501 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
     7561 acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gaaattccga
     7621 caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
     7681 attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
     7741 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
     7801 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
     7861 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
     7921 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
     7981 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
     8041 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
     8101 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
     8161 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
     8221 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
     8281 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
     8341 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
     8401 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
     8461 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
     8521 taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct
     8581 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
     8641 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca
     8701 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     8761 actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac
     8821 aattctcatg tttgacagct t
//
