LOCUS       sequence-436555-        7265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of mLemon in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Fraikin N, Couturier A, Mercier R, Lesterlin C
  TITLE     A palette of bright and photostable monomeric fluorescent proteins 
            for bacterial time-lapse imaging.
  JOURNAL   Sci Adv. 2025 Apr 18;11(16):eads6201. doi: 10.1126/sciadv.ads6201. 
            Epub 2025 Apr 16.
  PUBMED    40238862
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-04-18"; volume: "11(16):eads6201. doi"; pages: " 
            10.1126/sciadv.ads6201. Epub 2025 Apr 16"
COMMENT     Sequence Label: pNF02-mLemon
FEATURES             Location/Qualifiers
     source          1..7265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             218..229
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      974..1001
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1126..1599
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(1672..2643)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(2643..3818)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALNRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    4144..4394
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(4397..5152)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      5243..5462
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     promoter        6389..6491
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6492..7151
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6558..6577)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtcgacctgt aacagagcat tagcgcaagg tgatttttgt cttcttgcgc taatttttta
       61 cgcgtcacag ctaacaccac gtcgtcccta tctgctgccc taggtctatg agtggttgct
      121 ggataacttt acgggcatgc ataaggctcg tataatatat tcagggagac cacaacggtt
      181 tccctctaca aataattttg tttaactttt actagagaaa gaggagaaag ctagcatgtc
      241 taaaggtgaa gaactgttca ccggtgttgt tccgatcctg gttgaactgg atggtgatgt
      301 taacggccac aaattctctg ttcgtggtga aggtgaaggt gatgcaacca tcggtaaact
      361 gaccctgaaa ttcatctgca ctaccggtaa actgccggtt ccatggccga ctctggtgac
      421 cagcctgggc tatggtgttc agtgttttgc tcgttacccg gatcacatga agcagcatga
      481 tttcttcaaa tctgcaatgc cggaaggtta tgtacaggag cgcaccattt ctttcaaaga
      541 cgatggcacc tacaaaaccc gtgcagaggt taaatttgaa ggtgatactc tggtgaaccg
      601 tattgaactg aaaggcagtg gtttcaaaga ggacggcaac atcctgggcc acaaactgga
      661 atataactac aactcccata aagtttacat caccgcagac aaacagaaga acggtatcaa
      721 agctaacttc aaaattcgcc ataacgttga agacggtagc gtacagctgg cggaccacta
      781 ccagcagaac actccgatcg gtgatggtcc ggttctgctg ccggataacc actacctgtc
      841 ctaccagtct aaactgtcca aagacccgaa cgaaaagcgc gaccacatgg tgctgctgga
      901 gttcctgact gcagcaggta tcacgcacgg catggatgag ctctacaaat aagtgcacct
      961 ctagtatcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagaga
     1021 atataaaaag ccagattatt aatccggctt ttttattatt tacatgtcaa tacttgccct
     1081 tgacaggcat tgatggaatc gtagtctcac gctgatagtc tgatcgacaa tacaagtggg
     1141 accgtggtcc cagaccgata atcagaccga caacacgagt gggatcgtgg tcccagacta
     1201 ataatcagac cgacgatacg agtgggaccg tggtcccaga ctaataatca gaccgacgat
     1261 acgagtggga ccgtggttcc agactaataa tcagaccgac gatacgagtg ggaccgtggt
     1321 cccagactaa taatcagacc gacgatacga gtgggaccat ggtcccagac taataatcag
     1381 accgacgata cgagtgggac cgtggtccca gtctgattat cagaccgacg atacgagtgg
     1441 gaccgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact
     1501 aataatcaga ccgacgatac gagtgggacc gtggtcccag tctgattatc agaccgacga
     1561 tacaagtgga acagtgggcc cagagagaat attcaggcca gttatgcttt ctggcctgta
     1621 acaaaggaca ttaagtaaag acagataaac gtagactaaa acgtggtcgc atcagggtgc
     1681 tggcttttca agttccttaa gaatggcctc aattttctct atacactcag ttggaacacg
     1741 agacctgtcc aggttaagca ccattttatc gcccttatac aatactgtcg ctccaggagc
     1801 aaactgatgt cgtgagctta aactagttct tgatgcagat gacgttttaa gcacagaagt
     1861 taaaagagtg ataacttctt cagcttcaaa tatcacccca gcttttttct gctcatgaag
     1921 gttagatgcc tgctgcttaa gtaattcctc tttatctgta aaggcttttt gaagtgcatc
     1981 acctgaccgg gcagatagtt caccggggtg agaaaaaaga gcaacaactg atttaggcaa
     2041 tttggcggtg ttgatacagc gggtaataat cttacgtgaa atattttccg catcagccag
     2101 cgcagaaata tttccagcaa attcattctg caatcggctt gcataacgct gaccacgttc
     2161 ataagcactt gttgggcgat aatcgttacc caatctggat aatgcagcca tctgctcatc
     2221 atccagctcg ccaaccagaa cacgataatc actttcggta agtgcagcag ctttacgacg
     2281 gcgactccca tcggcaattt ctatgacacc agatactctt cgaccgaacg ccggtgtctg
     2341 ttgaccagtc agtagaaaag aagggatgag atcatccagt gcgtcctcag taagcagctc
     2401 ctggtcacgt tcattacctg accatacccg agaggtcttc tcaacactat caccccggag
     2461 cacttcaaga gtaaacttca catcccgacc acatacaggc aaagtaatgg cattaccgcg
     2521 agccattact cctacgcgcg caattaacga atccaccatc ggggcagctg gtgtcgataa
     2581 cgaagtatct tcaaccggtt gagtattgag cgtatgtttt ggaataacag gcgcacgctt
     2641 cattatctaa tctcccagcg tggtttaatc agacgatcga aaatttcatt gcagacaggt
     2701 tcccaaatag aaagagcatt tctccaggca ccagttgaag agcgttgatc aatggcctgt
     2761 tcaaaaacag ttctcatccg gatctgacct ttaccaactt catccgtttc acgtacaaca
     2821 ttttttagaa ccatgcttcc ccaggcatcc cgaatttgct cctccatcca cggggactga
     2881 gagccattac tattgctgta tttggtaagc aaaatacgta catcaggctc gaacccttta
     2941 agatcaacgt tcttgagcag atcacgaagc atatcgaaaa actgcagtgc ggaggtgtag
     3001 tcaaacaact cagcaggcgt gggaacaatc agcacatcag cagcacatac gacattaatc
     3061 gtgccgatac ccaggttagg cgcgctgtca ataactatga catcatagtc atgagcaaca
     3121 gtttcaatgg ccagtcggag catcaggtgt ggatcggtgg gcagtttacc ttcatcaaat
     3181 ttgcccatta actcagtttc aatacggttc agagccagac aggaaggaat aatgtcaagc
     3241 cccggccagc aagtgggctt tattgcataa gtgacatcgt ccttttcccc aagatagaaa
     3301 ggcaggagag tgtcttctgc atgaatatga agatctggta cccatccgtg atacattgag
     3361 gctgttccct gggggtcgtt accttccacg agcaaaacac gtagcccctt cagagccaga
     3421 tcctgagcaa gatgaacaga aactgaggtt ttgtaaacgc cacctttatg ggcagcaacc
     3481 ccgatcaccg gtggaaatac gtcttcagca cgtcgcaatc gcgtaccaaa cacatcacgc
     3541 atatgattaa tttgttcaat tgtataacca acacgttgct caacccgtcc tcgaatttcc
     3601 atatccgggt gcggtagtcg ccctgctttc tcggcatctc tgatagcctg agaagaaacc
     3661 ccaactaaat ccgctgcttc acctattctc cagcgccggg ttattttcct cgcttccggg
     3721 ctgtcatcat taaactgtgc aatggcgata gccttcgtca tttcatgacc agcgtttatg
     3781 cactggttaa gtgtttccat gagtttcatt ctgaacatcc tttaatcatt gctttgcgtt
     3841 tttttattaa atcttgcaat ttactgcaaa gcaacaacaa aatcgcaaag tcatcaaaaa
     3901 accgcaaagt tgtttaaaat aagagcaaca ctacaaaagg agataagaag agcacatacc
     3961 tcagtcactt attatcacta gcgctcgccg cagccgtgta accgagcata gcgagcgaac
     4021 tggcgaggaa gcaaagaaga actgttctgt cagatagctc ttacgctcag cgcaagaaga
     4081 aatatccacc gtgggaaaaa ctccaggtag aggtacacac gcggatagcc aattcagagt
     4141 aataaactgt gataatcaac cctcatcaat gatgacgaac taacccccga tatcaggtca
     4201 catgacgaag ggaaagagaa ggaaatcaac tgtgacaaac tgccctcaaa tttggcttcc
     4261 ttaaaaatta cagttcaaaa agtatgagaa aatccatgca ggctgaagga aacagcaaaa
     4321 ctgtgacaaa ttaccctcag taggtcagaa caaatgtgac gaaccaccct caaatctgtg
     4381 acagataacc ctcagactat cctgtcgtca tggaagtgat atcgcggaag gaaaatacga
     4441 tatgagtcgt ctggcggcct ttctttttct caatgtatga gaggcgcatt ggagttctgc
     4501 tgttgatctc attaacacag acctgcagga agcggcggcg gaagtcaggc atacgctggt
     4561 aactttgagg cagctggtaa cgctctatga tccagtcgat tttcagagag acgatgcctg
     4621 agccatccgg cttacgatac tgacacaggg attcgtataa acgcatggca tacggattgg
     4681 tgatttcttt tgtttcacta agccgaaact gcgtaaaccg gttctgtaac ccgataaaga
     4741 agggaatgag atatgggttg atatgtacac tgtaaagccc tctggatgga ctgtgcgcac
     4801 gtttgataaa ccaaggaaaa gattcatagc ctttttcatc gccggcatcc tcttcagggc
     4861 gataaaaaac cacttccttc cccgcgaaac tcttcaatgc ctgccgtata tccttactgg
     4921 cttccgcaga ggtcaatccg aatatttcag catatttagc aacatggatc tcgcagatac
     4981 cgtcatgttc ctgtagggtg ccatcagatt ttctgatctg gtcaacgaac agatacagca
     5041 tacgtttttg atcccgggag agactatatg ccgcctcagt gaggtcgttt gactggacga
     5101 ttcgcgggct atttttacgt ttcttgtgat tgataaccgc tgtttccgcc atgacagatc
     5161 catgtgaagt gtgacaagtt tttagattgt cacactaaat aaaaaagagt caataagcag
     5221 ggataacttt gtgaaaaaac agcttcttct gagggcaatt tgtcacaggg ttaagggcaa
     5281 tttgtcacag acaggactgt catttgaggg tgatttgtca cactgaaagg gcaatttgtc
     5341 acaacacctt ctctagaacc agcatggata aaggcctaca aggcgctcta aaaaagaaga
     5401 tctaaaaact ataaaaaaaa taattataaa aatatccccg tggataagtg gataacccca
     5461 agggaagttt tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt ccctggtgct
     5521 tcctcgctca ctcgagggct tcgccctgtc gctcgactgc ggcgagcact actggctgta
     5581 aaaggacaga ccacatcatg gttctgtgtt cattaggttg ttctgtccat tgctgacata
     5641 atccgctcca cttcaacgta acaccgcacg aagatttcta ttgttcctga aggcatattc
     5701 aaatcgtttt cgttaccgct tgcaggcatc atgacagaac actacttcct ataaacgcta
     5761 cacaggctcc tgagattaat aatgcggatc tctacgataa tgggagattt tcccgactgt
     5821 ttcgttcgct tctcagtgga taacagccag cttctctgtt taacagacaa aaacagcata
     5881 tccactcagt tccacatttc catataaagg ccaaggcatt tattctcagg ataattgttt
     5941 cagcatcgca accgcatcag actccggcat cgcaaactgc acccggtgcc gggcagccac
     6001 atccagcgca aaaaccttcg tgtagacttc cgttgaactg atggacttat gtcccatcag
     6061 gctttgcaga actttcagcg gtataccggc atacagcatg tgcatcgcat aggaatggcg
     6121 gaacgtatgt ggtgtgaccg gaacagagaa cgtcacaccg tcagcagcag cggcggcaac
     6181 cgcctcccca atccaggtcc tgaccgttct gtccgtcact tcccagatcc gcgctttctc
     6241 tgtccttcct gtgcgacggt tacgccgctc catgagctta tcgcgaataa atacctgtga
     6301 cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct
     6361 gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc
     6421 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
     6481 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     6541 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     6601 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     6661 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     6721 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     6781 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     6841 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     6901 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     6961 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     7021 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     7081 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     7141 gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt tgctacgcct
     7201 gaataagtga taataagcgg atgaatggca gaaattcgat gataagctgt caaacatgag
     7261 aattg
//
