LOCUS       sequence-436563-        7253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of mLychee in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Fraikin N, Couturier A, Mercier R, Lesterlin C
  TITLE     A palette of bright and photostable monomeric fluorescent proteins 
            for bacterial time-lapse imaging.
  JOURNAL   Sci Adv. 2025 Apr 18;11(16):eads6201. doi: 10.1126/sciadv.ads6201. 
            Epub 2025 Apr 16.
  PUBMED    40238862
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-04-18"; volume: "11(16):eads6201. doi"; pages: " 
            10.1126/sciadv.ads6201. Epub 2025 Apr 16"
COMMENT     Sequence Label: pNF02-mLychee
FEATURES             Location/Qualifiers
     source          1..7253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             218..229
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      962..989
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1114..1587
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(1660..2631)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(2631..3806)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGERDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    4132..4382
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(4385..5140)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      5231..5450
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     promoter        6377..6479
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6480..7139
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6546..6565)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtcgacctgt aacagagcat tagcgcaagg tgatttttgt attcttgcgc taatttttta
       61 cgcgtcacag ctaacaccac gtcgtcccta tctgctgccc taggtctatg agtggttgct
      121 ggataacttt acgggcatgc ataaggctcg tataatatat tcagggagac cacaacggtt
      181 tccctctaca aataattttg tttaactttt actagagaaa gaggagaaag ctagcatgga
      241 ttcaacagaa gcaattatta aagagtttat gcgttttaaa gtgcatatgg aaggctcagt
      301 taacggacac gagtttgaaa tcgagggtga aggtgaaggg cgtccgtatg aagccttcca
      361 gacagcaaaa ctgaaagtaa ctaaaggcgg gccgctgccg ttcgcctggg atatcctgag
      421 ccctcaattc atgtatggca gtaaggccta tatcaaacac ccggccgaca ttccggatta
      481 cttcaaacag tcgtttcctg aaggcttccg ctgggagcgt gttatgaact ttgaggatgg
      541 aggcattatc cacgtcaatc aggactcgag cttgcaggac ggcgtcttca tttacaaagt
      601 caaactgcgt ggcacaaact ttccaccaga tggcccggtg atgcagaagc gtactatggg
      661 atgggaaccc agtgaagagc gcatgtaccc tgaagacggc gcactgaaga gtgaaatcaa
      721 gaagcgtctg aaactgaaag acggcggtca ctacgctgct gaagtaaaaa ctacgtacaa
      781 agccaagaaa ccggtgcagc tgcccggtgc gtacatcgtc gatatcaagc tggacattgt
      841 cagccacaat gaagactaca ctgttgtcga gcagtatgag cgtgccgaag ggcgtcattc
      901 aggttcacaa ggcggctctg gcggaagttt gtacaagtaa gtgcacctct agtatcacac
      961 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc
     1021 agattattaa tccggctttt ttattattta catgtcaata cttgcccttg acaggcattg
     1081 atggaatcgt agtctcacgc tgatagtctg atcgacaata caagtgggac cgtggtccca
     1141 gaccgataat cagaccgaca acacgagtgg gatcgtggtc ccagactaat aatcagaccg
     1201 acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc
     1261 gtggttccag actaataatc agaccgacga tacgagtggg accgtggtcc cagactaata
     1321 atcagaccga cgatacgagt gggaccatgg tcccagacta ataatcagac cgacgatacg
     1381 agtgggaccg tggtcccagt ctgattatca gaccgacgat acgagtggga ccgtggtccc
     1441 agactaataa tcagaccgac gatacgagtg ggaccgtggt cccaaactaa taatcagacc
     1501 gacgatacga gtgggaccgt ggtcccagtc tgattatcag accgacgata caagtggaac
     1561 agtgggccca gagagaatat tcaggccagt tatgctttct ggcctgtaac aaaggacatt
     1621 aagtaaagac agataaacgt agactaaaac gtggtcgcat cagggtgctg gcttttcaag
     1681 ttccttaaga atggcctcaa ttttctctat acactcagtt ggaacacgag acctgtccag
     1741 gttaagcacc attttatcgc ccttatacaa tactgtcgct ccaggagcaa actgatgtcg
     1801 tgagcttaaa ctagttcttg atgcagatga cgttttaagc acagaagtta aaagagtgat
     1861 aacttcttca gcttcaaata tcaccccagc ttttttctgc tcatgaaggt tagatgcctg
     1921 ctgcttaagt aattcctctt tatctgtaaa ggctttttga agtgcatcac ctgaccgggc
     1981 agatagttca ccggggtgag aaaaaagagc aacaactgat ttaggcaatt tggcggtgtt
     2041 gatacagcgg gtaataatct tacgtgaaat attttccgca tcagccagcg cagaaatatt
     2101 tccagcaaat tcattctgca atcggcttgc ataacgctga ccacgttcat aagcacttgt
     2161 tgggcgataa tcgttaccca atctggataa tgcagccatc tgctcatcat ccagctcgcc
     2221 aaccagaaca cgataatcac tttcggtaag tgcagcagct ttacgacggc gactcccatc
     2281 ggcaatttct atgacaccag atactcttcg accgaacgcc ggtgtctgtt gaccagtcag
     2341 tagaaaagaa gggatgagat catccagtgc gtcctcagta agcagctcct ggtcacgttc
     2401 attacctgac catacccgag aggtcttctc aacactatca ccccggagca cttcaagagt
     2461 aaacttcaca tcccgaccac atacaggcaa agtaatggca ttaccgcgag ccattactcc
     2521 tacgcgcgca attaacgaat ccaccatcgg ggcagctggt gtcgataacg aagtatcttc
     2581 aaccggttga gtattgagcg tatgttttgg aataacaggc gcacgcttca ttatctaatc
     2641 tcccagcgtg gtttaatcag acgatcgaaa atttcattgc agacaggttc ccaaatagaa
     2701 agagcatttc tccaggcacc agttgaagag cgttgatcaa tggcctgttc aaaaacagtt
     2761 ctcatccgga tctgaccttt accaacttca tccgtttcac gtacaacatt ttttagaacc
     2821 atgcttcccc aggcatcccg aatttgctcc tccatccacg gggactgaga gccattacta
     2881 ttgctgtatt tggtaagcaa aatacgtaca tcaggctcga accctttaag atcaacgttc
     2941 ttgagcagat cacgaagcat atcgaaaaac tgcagtgcgg aggtgtagtc aaacaactca
     3001 gcaggcgtgg gaacaatcag cacatcagca gcacatacga cattaatcgt gccgataccc
     3061 aggttaggcg cgctgtcaat aactatgaca tcatagtcat gagcaacagt ttcaatggcc
     3121 agtcggagca tcaggtgtgg atcggtgggc agtttacctt catcaaattt gcccattaac
     3181 tcagtttcaa tacggtgcag agccagacag gaaggaataa tgtcaagccc cggccagcaa
     3241 gtgggcttta ttgcataagt gacatcgtcc ctttccccaa gatagaaagg caggagagtg
     3301 tcttctgcat gaatatgaag atctggtacc catccgtgat acattgaggc tgttccctgg
     3361 gggtcgttac cttccacgag caaaacacgt agccccttca gagccagatc ctgagcaaga
     3421 tgaacagaaa ctgaggtttt gtaaacgcca cctttatggg cagcaacccc gatcaccggt
     3481 ggaaatacgt cttcagcacg tcgcaatcgc gtaccaaaca catcacgcat atgattaatt
     3541 tgttcaattg tataaccaac acgttgctca acccgtcctc gaatttccat atccgggtgc
     3601 ggtagtcgcc ctgctttctc ggcatctctg atagcctgag aagaaacccc aactaaatcc
     3661 gctgcttcac ctattctcca gcgccgggtt attttcctcg cttccgggct gtcatcatta
     3721 aactgtgcaa tggcgatagc cttcgtcatt tcatgaccag cgtttatgca ctggttaagt
     3781 gtttccatga gtttcattct gaacatcctt taatcattgc tttgcgtttt tttattaaat
     3841 cttgcaattt actgcaaagc aacaacaaaa tcgcaaagtc atcaaaaaac cgcaaagttg
     3901 tttaaaataa gagcaacact acaaaaggag ataagaagag cacatacctc agtcacttat
     3961 tatcactagc gctcgccgca gccgtgtaac cgagcatagc gagcgaactg gcgaggaagc
     4021 aaagaagaac tgttctgtca gatagctctt acgctcagcg caagaagaaa tatccaccgt
     4081 gggaaaaact ccaggtagag gtacacacgc ggatagccaa ttcagagtaa taaactgtga
     4141 taatcaaccc tcatcaatga tgacgaacta acccccgata tcaggtcaca tgacgaaggg
     4201 aaagagaagg aaatcaactg tgacaaactg ccctcaaatt tggcttcctt aaaaattaca
     4261 gttcaaaaag tatgagaaaa tccatgcagg ctgaaggaaa cagcaaaact gtgacaaatt
     4321 accctcagta ggtcagaaca aatgtgacga accaccctca aatctgtgac agataaccct
     4381 cagactatcc tgtcgtcatg gaagtgatat cgcggaagga aaatacgata tgagtcgtct
     4441 ggcggccttt ctttttctca atgtatgaga ggcgcattgg agttctgctg ttgatctcat
     4501 taacacagac ctgcaggaag cggcggcgga agtcaggcat acgctggtaa ctttgaggca
     4561 gctggtaacg ctctatgatc cagtcgattt tcagagagac gatgcctgag ccatccggct
     4621 tacgatactg acacagggat tcgtataaac gcatggcata cggattggtg atttcttttg
     4681 tttcactaag ccgaaactgc gtaaaccggt tctgtaaccc gataaagaag ggaatgagat
     4741 atgggttgat atgtacactg taaagccctc tggatggact gtgcgcacgt ttgataaacc
     4801 aaggaaaaga ttcatagcct ttttcatcgc cggcatcctc ttcagggcga taaaaaacca
     4861 cttccttccc cgcgaaactc ttcaatgcct gccgtatatc cttactggct tccgcagagg
     4921 tcaatccgaa tatttcagca tatttagcaa catggatctc gcagataccg tcatgttcct
     4981 gtagggtgcc atcagatttt ctgatctggt caacgaacag atacagcata cgtttttgat
     5041 cccgggagag actatatgcc gcctcagtga ggtcgtttga ctggacgatt cgcgggctat
     5101 ttttacgttt cttgtgattg ataaccgctg tttccgccat gacagatcca tgtgaagtgt
     5161 gacaagtttt tagattgtca cactaaataa aaaagagtca ataagcaggg ataactttgt
     5221 gaaaaaacag cttcttctga gggcaatttg tcacagggtt aagggcaatt tgtcacagac
     5281 aggactgtca tttgagggtg atttgtcaca ctgaaagggc aatttgtcac aacaccttct
     5341 ctagaaccag catggataaa ggcctacaag gcgctctaaa aaagaagatc taaaaactat
     5401 aaaaaaaata attataaaaa tatccccgtg gataagtgga taaccccaag ggaagttttt
     5461 tcaggcatcg tgtgtaagca gaatatataa gtgctgttcc ctggtgcttc ctcgctcact
     5521 cgagggcttc gccctgtcgc tcgactgcgg cgagcactac tggctgtaaa aggacagacc
     5581 acatcatggt tctgtgttca ttaggttgtt ctgtccattg ctgacataat ccgctccact
     5641 tcaacgtaac accgcacgaa gatttctatt gttcctgaag gcatattcaa atcgttttcg
     5701 ttaccgcttg caggcatcat gacagaacac tacttcctat aaacgctaca caggctcctg
     5761 agattaataa tgcggatctc tacgataatg ggagattttc ccgactgttt cgttcgcttc
     5821 tcagtggata acagccagct tctctgttta acagacaaaa acagcatatc cactcagttc
     5881 cacatttcca tataaaggcc aaggcattta ttctcaggat aattgtttca gcatcgcaac
     5941 cgcatcagac tccggcatcg caaactgcac ccggtgccgg gcagccacat ccagcgcaaa
     6001 aaccttcgtg tagacttccg ttgaactgat ggacttatgt cccatcaggc tttgcagaac
     6061 tttcagcggt ataccggcat acagcatgtg catcgcatag gaatggcgga acgtatgtgg
     6121 tgtgaccgga acagagaacg tcacaccgtc agcagcagcg gcggcaaccg cctccccaat
     6181 ccaggtcctg accgttctgt ccgtcacttc ccagatccgc gctttctctg tccttcctgt
     6241 gcgacggtta cgccgctcca tgagcttatc gcgaataaat acctgtgacg gaagatcact
     6301 tcgcagaata aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt
     6361 ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
     6421 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
     6481 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     6541 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     6601 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     6661 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     6721 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     6781 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     6841 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     6901 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     6961 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     7021 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     7081 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
     7141 ttttttaagg cagttattgg tgcccttaaa cgcctggttg ctacgcctga ataagtgata
     7201 ataagcggat gaatggcaga aattcgatga taagctgtca aacatgagaa ttg
//
