LOCUS       sequence-436564-        3375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CDS of H3H from N. nambi codon optimized for N.benthamiana. This 
            gene is the second step of the bioluminiscente pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB3472
FEATURES             Location/Qualifiers
     source          1..3375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1332..1389
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1413..1430)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1584..2172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1664..1683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2355..2449)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2470..3129)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3044..3063
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3291..3309
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3313..3356)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(3359..3375,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg aatggcatcc tttgaaaata gtctgtccgt tttgatcgtc
       61 ggggctggcc ttgggggatt agcagccgca atagctctta ggagacaggg ccatgtagta
      121 aagatctatg atagttcctc attcaaagct gaactgggtg ctggccttgc tgtacctccg
      181 aatacgctta gaagccttca acagttaggt tgtaatacag agaatttgaa cggagtcgac
      241 aatctctgct tcacggccat gggttacgac ggctccgtag gtatgatgaa taacatgaca
      301 gactacaggg aggcttatgg cacatcctgg attatggttc atcgagtgga tttacataat
      361 gaacttatgc gtgtcgcact tgatccgggg gggctgggac caccagcaac attacattta
      421 aatcacagag tgaccttctg cgacgttgac gcatgtacgg taacttttac caacgggacg
      481 acacaaagtg ccgacttgat cgtaggggct gacggcatac gaagcaccat acgtagattc
      541 gtcttggagg aagatgtaac tgtgcctgcc agcgggatcg tcggtttcag atggctggtg
      601 caagccgatg ccttagaccc ttaccctgag ttagattgga ttgttaagaa gcctccactc
      661 ggtgctcgtc tgatctctac gccacagaat ccacagtccg gcgtggggtt ggctgaccgt
      721 cgtacaatta tcatctacgc ttgtaggggt ggcacgatgg ttaatgtgtt ggctgtccac
      781 gacgacgaaa gagatcagaa tacggcagat tggtcagtgc cggcttccaa agatgatctc
      841 ttccgtgtat tccatgacta ccacccaaga ttcagacgtt tgctcgaact tgctcaagat
      901 atcaatctct ggcaaatgag ggtagttccc gtgcttaaaa aatgggtgaa caagcgtgtc
      961 tgtttattag gagatgccgc ccatgcttct ctccccacgc tgggtcaagg gttcggaatg
     1021 ggactggagg acgccgtggc actcggcaca ttgttaccca aaggaactac ggcttcacag
     1081 atcgaaacga gactggccgt ttacgagcaa ttacgaaagg atagggctga gttcgtagca
     1141 gcagaatcct acgaggaaca gtatgtccct gagatgcgtg ggctctatct tcgttccaaa
     1201 gaactccgag atcgagtgat gggatatgat attaaagtgg agtcagaaaa ggttttggag
     1261 actctgcttc gatcctctaa ttctgcatga gctttgagac cactcatcgc gactagtagc
     1321 ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
     1381 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
     1441 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1501 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1561 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
     1621 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1681 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1741 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1801 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1861 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1921 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1981 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2041 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2101 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2161 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2221 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2281 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2341 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     2401 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     2461 gatatcaaat tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat
     2521 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     2581 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     2641 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa
     2701 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     2761 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga
     2821 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     2881 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     2941 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     3001 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     3061 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     3121 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     3181 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag
     3241 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3301 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga
     3361 attcgcggcc gcttc
//
