LOCUS       sequence-436566-        2910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CDS of Luz gene from N. nambi codon optimized for N.benthamiana. 
            This gene generate the luminiscence of the bioluminiscente pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB3473
FEATURES             Location/Qualifiers
     source          1..2910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(18..112)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(133..792)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     707..726
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     954..972
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(976..1019)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1022..1043
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(1356..1367)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      1905..1962
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1986..2003)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2157..2745)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2237..2256)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
       61 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
      121 ctcgatatca aattacgccc cgccctgcca ctcgtcgcag tactgttgta attcattaag
      181 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
      241 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
      301 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgatac
      361 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
      421 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
      481 tgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
      541 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
      601 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
      661 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
      721 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
      781 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
      841 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc
      901 gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
      961 gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct
     1021 ggaattcgcg gccgcttcta gagcgatgag tggtctcgaa tgagaattaa catctccctt
     1081 tcatcattat ttgaacgtct gtcaaagtta tccagcagat ccattgcaat cacctgtggt
     1141 gtcgtcttgg cctccgcaat cgcttttccg ataatccgta gggactatca gacttttttg
     1201 gaagtcgggc cgagttacgc tccgcagaat ttcagaggtt acatcatagt atgcgtgctt
     1261 tcactcttta ggcaggaaca aaaagggttg gctatctatg atcgactgcc cgaaaagcga
     1321 aggtggttgg cagatctccc attccgagaa ggtacgcgtc cttctatcac aagtcatatc
     1381 atccaaagac agaggactca gctggtagac caagaatttg caacccgaga actcattgat
     1441 aaagttatac ccagagttca ggctcgacac accgataaga cgtttctgtc aacctcaaag
     1501 ttcgaatttc atgccaaagc aatttttttg ctgcccagta ttcccatcaa tgatcccttg
     1561 aatatacctt cacatgatac tgtcagacga acaaaaaggg agatcgctca catgcacgat
     1621 taccacgact gcaccctgca cttggctctc gcagcccagg acggtaaaga agtacttaaa
     1681 aaagggtggg gccagagaca tcccctcgct ggccccggcg tgccgggacc tccaaccgaa
     1741 tggacgtttt tatatgcacc gcgtaacgag gaggaagcca gagtggtcga gatgatagtg
     1801 gaggccagta ttggttacat gacaaatgac cccgctggta aaattgtaga gaacgctaag
     1861 tgagctttga gaccactcat cgcgactagt agcggccgct gcagtccggc aaaaaagggc
     1921 aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttatgcag
     1981 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2041 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2101 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2161 cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2221 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2281 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2341 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2401 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2461 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2521 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2581 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2641 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2701 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2761 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2821 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2881 atctaaagta tatatgagta aacttggtct
//
