LOCUS       sequence-43658-e        5718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+CeGFP-ABCC9a
FEATURES             Location/Qualifiers
     source          1..5718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4794..5507
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4824..4852)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5447..5467
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    5561..5695
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5585..5604)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5639..5658
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctagtatggg atcagatgat tgggagtggt tctgtggtac aaacagcagt gacctgccag
       61 actttgataa ccagtcttgg aactatgagg atgtgttgag taatgagtgt ttggtggatg
      121 ctttcatcgc cgggactcag atagggttct tcgtcctagc tcttctagtt ctctttctca
      181 ccgcatgctg cacctcacgc agtgatgtac cacatcggtt cctgctccgt tacgtaacac
      241 atagtcctag atatcttggt acctttgttc tggtcgtgat gtcagtggca tctgttgcgg
      301 aaggtatcct gactgatagc acatatacca accaacctac acaaccacat ctctacatta
      361 caggcatatc atctgcgtta agtggagttg tatccctgct ctactttggt cgtctcgaga
      421 gatggcgtgt ttggaagatg tctctcctcc tcctcgtcta ctgggtggct tccttggcaa
      481 ccgtaagcat ccagattgag tccctcatcc atcagggata caacgatgtt catgtgacag
      541 ccttcgacat caatatcatc atgattgcag tatacagctt cttcttactc ctcgagctgt
      601 atttcctcgt agacaactgg tttggttgga gtcccggcgt ggatgaagtg cctcgagact
      661 taaaggatga acgcatgtac tacatggagc gctacgtcga catcttctct cagcttgtcc
      721 attggtggat gaactggctc ttcgttctcg gtttcaagag gccactcgag atggatgatc
      781 tgggaagcat accggaggtc cacagcgccg agtacaatca tagacggttc aagaacaact
      841 tcaagagaga gatagagcgg gcaaaggaag aagagcggga accgagtttg ttcaaggtgt
      901 attttgccac ctactgggtc cgaatgctgg ccggtgctat cctcaagttc atcacagaca
      961 tgggagccta tgtgggtccg ttggccattg gaggcatcac tctttatgtc accgatgctg
     1021 tctacggtga acatgaagac gtaccacccc acccggtgag cgtaaaagag ttcttcgcag
     1081 atggttacgt tctgatcggt tgtatattcg tcactgctct tataagacac atgtgtgacc
     1141 aaacctatca gtttatcacc tccatagaag gcatccatgc taggtcggcc cttcagagta
     1201 cgatctacga gaaatcctta cgactgtcta catacgccat gactggaggg gcgatgacca
     1261 tgggacagat caccaatcac atgtcagtag atgccaacaa tatcatgatg ttcttcatgt
     1321 ttgctaacga agtctgggca actccgttca agatcatatt ttccttggta ctgttatacc
     1381 ttgagctggg tacaccagca ttgatcggtg ccagtgtctt tctagttgtg atccctttcc
     1441 aactagctgc tgccgtcgct atgggaaaat tcatcaaggc tgttttgggt acggcggact
     1501 accgtctgaa gatgtcaaac gagatgctac agggaatcaa actcttgaag ctttatggat
     1561 gggagaggat atactatgaa gcaatcaaga aggcgagaat caaggaactt tatgctcttc
     1621 taaaggtcta cacattcttc gctgtaacgt ttatcatcaa tgctgcaact ccaatcgtcg
     1681 tcacccttgt gtctttcggg acatacacac ccatcacggg gaaggtcctc acacctgacg
     1741 tggcatttac aacgctctcg ctcttcaatc aaatggctgg tcctctcttc atgtttcctt
     1801 tcgtcatcaa tctctttgtc aactggatcg tgagtacagg acgtcttaag acgtatctcc
     1861 ttgcacccga ggttgaaggc gccgatggag tgatcagcga tctactgaag gagattgatg
     1921 aggatggcga tgtaacggac agtgccgaga gcgtgggtaa ttcacacgat gacgatcttt
     1981 cggtgaccat caagcatccc cggcacagga agaaccgaag tcatagcaaa cagagagacg
     2041 atgctcatca actccctgat aagacaccat tgttgagcgg ggacggatcg ccacgaggat
     2101 ctctctctta cggatctacc aatcccatca aacatggaga tacgtcattc agtggcagcg
     2161 gggatgtccc cgatgacgtc gccataaaga tctctgatgg taacttcact tgggatcctg
     2221 attccaatat atctatactg tccgacatca atgtagagat accaagaggt aagctaacag
     2281 tcgtgattgg ggcggttgga tgcggaaaat catcccttct cagtgccatg ttgaatgaaa
     2341 tgacaacact ctccggcacc gttaagcaca acagaaacac gaaatccatt gcatacgccc
     2401 cacagaaagc atggttggtc aatgctacgc tcaaagacaa catattattt ggcgaagaga
     2461 tggttaacag caaatatcgc aaagtgatcc aagcatgcgc actgcagccc gacatagaca
     2521 tgttacctgg taaagacatg acagagattg gcgagaaagg gatcaacctg agcggagggc
     2581 agaagcaacg cgtcagtgtc agccgatgtc tctactccga cagggacatc atcatcttgg
     2641 acgaccctct ctctgctctg gacatgcacg ttggcacgca tctgttcgag aaaggattcc
     2701 aaggtatctt gatgaaacaa aagaagacag tcatactggt aacccatcag ctccagtatc
     2761 tgcctgaagc cgacaaaatc attgtggtga aggatggccg catcacttgc cagggtactc
     2821 ccgaggaggt agccgaggct gacccttcga tcatgactga gtctgagaga gctatcaagg
     2881 aagtgaccga atccgaagcc gaagggtctg gggcagagtc tgagtctctg atgaatgaga
     2941 gaagggcgct caagagacag atctcaacac agatgtctgt gttgaaggac ggcgctgctg
     3001 ataaatcaga cgagaaagca ggacgcttga ttgaagagga ggagaaggag aaaggttctg
     3061 tgtcttacca gatgtacatg tactacttcc gagccatggg ctattggctt gcatgtctca
     3121 tgatcggttg tgtagccatg cgagccggtc ttcaggtagc tacaaacttc tggctctcag
     3181 actggtccga ggcgggtctc aatttaactg gggacgccca accgacgagt tattatttga
     3241 gaggttatgc gggtttctct gtgtctactg tttttggatc cgttttatcg aatgtcttca
     3301 gctgtatcgg tgccttgcta gcggcaagag cgatacactt tgcccttcta caaaatatca
     3361 tcggaatacc aatgagattc tttgacacca ccccagttgg acgaattctc aaccgactct
     3421 ccagtgacac acaatggatc gatcagcgtt tgattcactc cttcaacatg cttctcaata
     3481 cgttagtcgc cgtcatcgcc tcctttatcg tcaacgccat cgtcaatagc tacttcatca
     3541 tcttcctcat ccctatcggc atcatcttca tgtgcattct ggtctacttc gtcgcaacat
     3601 ccagaatgtt gcagcgtttg gagagcgtca caagatctcc tgtgttcgct catttctctg
     3661 aaacgcttgg cggtctgccc atcatcagag catataagga tgaaaagcgg tttttcaaaa
     3721 acgtactggg gaaaatcgac aaaaacaata cagctttggt gtatttgtac acagccaaca
     3781 gatggttagc gattcgattg gattatctgg gagcagtggt tgtgctcatt gccagtctgt
     3841 cggcactctt gggggcgctc tatcttggtg tagatgctag tttggttggg cttgccatca
     3901 gctattcatt agagatctcc atctatatga acatggtggt gcgaggttgt gccgacttgg
     3961 agcttcagat gaacgccatg gagagaattc gatactacac tgaagtagag aatgaggaat
     4021 acgatggtct ggagcctcca cccgagtggc cccagaaagg tgaaatcatt cttgataaca
     4081 tcagcgtacg ttacgccctg gagctcgatc ctgtactgca tgatgtctct gtcaccatcc
     4141 ctcctggtca aaagttggga atctgtggtc gaaccgggag cggtaaatca tcgcttacac
     4201 tttctctctt ccgtatcatc gatactttca aaggtcgaat tctgattgac ggagttgata
     4261 tttcatcagt tcctctcatc acactccgtc aacgactttc tatcatccca caagatgccg
     4321 tcctcttcac tggaactatc agacataaca ttgatccagc gggtgtaaag acagacgatg
     4381 aactatggaa ggctttagag atagctcaac tcaaagaagt cgtttcaccg ttagaaggtg
     4441 gattggacta tatcgtgacg gagggaggag agaatttcag cgttggtcag cgacagctct
     4501 tctgtctggc ccgggctttc ctccgtaact caactatctt ggtcatggat gaagctactg
     4561 cttccatcga tcaagaaact gatcgcatcc tccaagacgt ggtggctgat gtcttccaag
     4621 atagaacagt catcacaatc gcgcatcgcg tgggtacgat cttggacgca gataacatcc
     4681 tcactctcag tgacggtcgc gtcgtcgagt tcgactcccc gaacacgctc ctcgaaaaag
     4741 aagacagcat cttcgcgtcg ctggtcaaag ctggcaaaac tagtcatcga ttgatgagta
     4801 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     4861 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
     4921 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
     4981 ctctcactta tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa cggcatgact
     5041 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     5101 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
     5161 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
     5221 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
     5281 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
     5341 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     5401 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     5461 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa gaattcaagg
     5521 cctctcgagc ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata
     5581 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     5641 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     5701 caacaattgc attcattt
//
