LOCUS       sequence-436582-       10134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TU for the expression of the CIB1 gene fused to the VPR activation 
            domain under the P35S promoter and TNOS terminator in alpha1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10134)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 10134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4193
FEATURES             Location/Qualifiers
     source          1..10134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        616..960
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     904..924
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1034..1543
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             2048..2068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2072..2092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2096..2116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2117..2266
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             2267..2287
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2291..2311
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2312..2668
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             2693..3262
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      3289..3541
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3576..3594)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3778..3802
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5103..5732
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6161..7234
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7300..7494
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7730..7752)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7838..7978
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7893..7912
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7993..8010)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8164..8752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8244..8263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8840..9655)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8840..9655)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9563..9582
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    10088..10112
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcccctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat tacaatttac
     1021 tattctagtc gaaatgaatg gagctatagg aggtgacctt ttgctcaatt ttcctgacat
     1081 gtcggtccta gagcgccaaa gggctcacct caagtacctc aatcccacct ttgattctcc
     1141 tctcgccggc ttctttgccg attcttcaat gattaccggc ggcgagatgg acagctatct
     1201 ttcgactgcc ggtttgaatc ttccgatgat gtacggtgag actacggtgg aaggtgattc
     1261 aagactctca atttcgccgg aaacgacgct tgggactgga aatttcaaga aacggaagtt
     1321 tgatacagag actaaggatt gtaatgagaa gaagaagaag atgacgatga acagagatga
     1381 cctagtagaa gaaggagaag aagagaagtc gaaaataaca gagcaaaaca atgggagcac
     1441 aaaaagcatc aagaagatga aacacaaagc caagaaagaa gagaacaatt tctctaatga
     1501 ttcatctaaa gtgacgaagg aattggagaa aacggattat attcatgttc gtgcacgacg
     1561 aggccaagcc actgatagtc acagcatagc agaacgagtt agaagagaaa agatcagtga
     1621 gagaatgaag tttctacaag atttggttcc tggatgcgac aagatcacag gcaaagcagg
     1681 gatgcttgat gaaatcatta actatgttca gtctcttcag agacaaatcg agttcttatc
     1741 gatgaaacta gcaattgtga atccaaggcc ggattttgat atggatgaca tttttgccaa
     1801 agaggttgcc tcaactccaa tgactgtggt gccatctcct gaaatggttc tttccggtta
     1861 ttctcatgag atggttcact ctggttattc tagtgagatg gttaactccg gttaccttca
     1921 tgtcaatcca atgcagcaag tgaataccag ttctgatcca ttgtcatgct tcaacaatgg
     1981 cgaagctcct tcgatgtggg actctcatgt gcagaatctc tatggcaatt taggagtagg
     2041 ttcggaccca aaaaagaagc gtaaggtcga tcccaagaag aagagaaagg tagatcctaa
     2101 gaagaagaga aaggtagacg cattggatga cttcgactta gacatgcttg ggtcagatgc
     2161 tttggacgac tttgacctcg acatgttggg ttctgacgcg ctagatgact tcgacttgga
     2221 catgttgggc tccgacgctt tggacgactt tgatctggac atgttaccga aaaagaaacg
     2281 caaagttgat cctaagaaga agagaaaggt acctacacag gccggcgagg gcacactgtc
     2341 tgaagctctg ctgcagctgc agttcgacga cgaggatctg ggagccctgc tgggaaacag
     2401 caccgatcct gccgtgttca ccgacctggc cagcgtggac aacagcgagt tccagcagct
     2461 gctgaaccag ggcatccctg tggcccctca caccaccgag cccatgctga tggaataccc
     2521 cgaggccatc acccggctcg tgacaggcgc tcagaggcct cctgatccag ctcctgcccc
     2581 tctgggagca ccaggcctgc ctaatggact gctgtctggc gacgaggact tcagctctat
     2641 cgccgatatg gatttctcag ccttgctgat taactctaga agttccggat ctcgggattc
     2701 cagggaaggg atgtttttgc cgaagcctga ggccggctcc gctattagtg acgtgtttga
     2761 gggccgcgag gtgtgccagc caaaacgaat ccggccattt catcctccag gaagtccatg
     2821 ggccaaccgc ccactccccg ccagcctcgc accaacacca accggtccag tacatgagcc
     2881 agtcgggtca ctgaccccgg caccagtccc tcagccactg gatccagcgc ccgcagtgac
     2941 tcccgaggcc agtcacctgt tggaggatcc cgatgaagaa acgagccagg ctgtcaaagc
     3001 ccttcgggag atggccgata ctgtgattcc ccagaaggaa gaggctgcaa tctgtggcca
     3061 aatggacctt tcccatccgc ccccaagggg ccatctggat gagctgacaa ccacacttga
     3121 gtccatgacc gaggatctga acctggactc acccctgacc ccggaattga acgagattct
     3181 ggataccttc ctgaacgacg agtgcctctt gcatgccatg catatcagca caggactgtc
     3241 catcttcgac acatctctgt tttgagcttg gaatggatct tcgatcccga tcgttcaaac
     3301 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgac gattatcata
     3361 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     3421 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     3481 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     3541 cgggaattgc caagctaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     3601 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     3661 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3721 acaataaccc tgataaatgc ttcaataatg ggaccgactc gcgcttgaga cgaagcttga
     3781 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
     3841 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     3901 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
     3961 ctgacgttca gtgcagccgt catctgaaaa cgacatgtcg cacaagtcct aagttacgcg
     4021 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
     4081 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
     4141 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
     4201 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
     4261 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac
     4321 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca
     4381 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc
     4441 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat
     4501 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg
     4561 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg
     4621 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact
     4681 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga
     4741 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc
     4801 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg
     4861 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acctctcaac cgtgcggctg
     4921 catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc
     4981 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg
     5041 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac
     5101 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa
     5161 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc
     5221 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg
     5281 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag
     5341 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact
     5401 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg
     5461 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt
     5521 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg
     5581 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg
     5641 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc
     5701 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa
     5761 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt
     5821 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga
     5881 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc
     5941 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt
     6001 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat
     6061 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc
     6121 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc
     6181 cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc
     6241 gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc
     6301 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat
     6361 taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt
     6421 gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga
     6481 ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc
     6541 agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca
     6601 tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt
     6661 ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa
     6721 agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac
     6781 gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg
     6841 ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga
     6901 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga
     6961 ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc
     7021 aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg
     7081 agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga
     7141 gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa
     7201 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat
     7261 tgccctagca ggggaaaaag gtcgaaaagg actctttcct gtggatagca cgtacattgg
     7321 gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat
     7381 tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc
     7441 ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc
     7501 tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct
     7561 acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct
     7621 acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg
     7681 cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     7741 tgcagctccc ggtgacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     7801 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     7861 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     7921 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     7981 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8041 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8101 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8161 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8221 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8281 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8341 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8401 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8461 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8521 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8581 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8641 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8701 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8761 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8821 ggtcatgcat tctaggtgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     8881 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     8941 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     9001 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     9061 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
     9121 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     9181 cgttattcat tcgtgattgc gcctgagcga gtcgaaatac gcgatcgctg ttaaaaggac
     9241 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     9301 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag
     9361 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     9421 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     9481 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cggtagattg
     9541 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     9601 tgttggaatt taatcgcggc cttgagcaag acgtttcccg ttgaatatgg ctcataacag
     9661 aacttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
     9721 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
     9781 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
     9841 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
     9901 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
     9961 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
    10021 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
    10081 tggctggtgg caggatatat tgtggtgtaa acataacaag cttcgtctca gtca
//
