LOCUS       sequence-43660-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS2a-RFP
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        203..258
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    204..222
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(350..369)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 cggcagcgga ccacctttgg taactttcag tttagcggtc tgggtacctt cgtacggacg
       61 accttcacct tcaccttcga tttcgaactc gtgaccgtta acggaacctt ccatacgaac
      121 tttgaaacgc atgaactctt tgataacgtc ttcgctactc gccatatgta tatctccttc
      181 ttaaaagatc ttttgaattc ttttctctat cactgatagg gagtggtaaa ataactctat
      241 caacgataga gtgtcaacaa aaattaggaa ttaatgatgt ctagattaga taaaagtaaa
      301 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
      361 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
      421 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
      481 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
      541 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
      601 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
      661 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
      721 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
      781 ttattacgac aagctatcga attatttgat ca
//
