LOCUS       sequence-43662-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS2c-RFP
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        198..253
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    199..217
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(345..364)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gcggaccacc tttggtaact ttcagtttag cggtctgggt accttcgtac ggacgacctt
       61 caccttcacc ttcgatttcg aactcgtgac cgttaacgga accttccata cgaactttga
      121 aacgcatgaa ctctttgata acgtcttcgc tactcgccat atgtatatct ccttcttaaa
      181 agatcttttg aattcttttc tctatcactg atagggagtg gtaaaataac tctatcaacg
      241 atagagtgtc aacaaaaatt aggaattaat gatgtctaga ttagataaaa gtaaagtgat
      301 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
      361 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
      421 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
      481 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
      541 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
      601 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
      661 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
      721 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
//
