LOCUS       sequence-43664-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS2k-RFP
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        188..243
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    189..207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(335..354)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 tttggtaact ttcagtttag cggtctgggt accttcgtac ggacgacctt caccttcacc
       61 ttcgatttcg aactcgtgac cgttaacgga accttccata cgaactttga aacgcatgaa
      121 ctctttgata acgtcttcgc tactcgccat atgtatatct ccttcttaaa agatcttttg
      181 aattcttttc tctatcactg atagggagtg gtaaaataac tctatcaacg atagagtgtc
      241 aacaaaaatt aggaattaat gatgtctaga ttagataaaa gtaaagtgat taacagcgca
      301 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
      361 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
      421 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
      481 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
      541 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
      601 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
      661 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
      721 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
      781 atcgaattat ttgatcacca aagtgcagag
//
