LOCUS       sequence-436649-        9264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for a subunit of rat TRPV1 with 
            circularly permutated HaloTag inserted between the S5 and S6 
            transmembrane helices.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Mott TM, Wulffraat GC, Eddins AJ, Mehl RA, Senning EN
  TITLE     Fluorescence labeling strategies for cell surface expression of 
            TRPV1.
  JOURNAL   J Gen Physiol. 2024 Oct 7;156(10):e202313523. doi: 
            10.1085/jgp.202313523. Epub 2024 Aug 20.
  PUBMED    39162763
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Physiol."; date: "2024-10-7"; volume: "156(10):e202313523. doi"; 
            pages: " 10.1085/jgp.202313523. Epub 2024 Aug 20"
COMMENT     Sequence Label: pcDNA3.1-TRPV1exCellHalo
FEATURES             Location/Qualifiers
     source          1..9264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    37..261
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     250..267
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        287..305
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     287..304
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4240..4259)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4241..4259)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4304..4507)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4333..4353)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4508..4887
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5059..5078
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5153..5257
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5258..5326,5327..6118)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5476..5495)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6289..6877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6778..6797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7031..7048
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7165..7186
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7201..7206,7207..7224,7225..7231)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7239..7255
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7244..7266
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7263..7279
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7263..7279
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7328..7449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7339..7358)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7393..7412
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7623..8417)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7734..7753)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8344..8363
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8484..8813)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8498..8633
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8552..8571)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8798..8818
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(8827..9255)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8937..8958)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9149..9168
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccctagagcc ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag
       61 catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
      121 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
      181 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg
      241 gctggcaact agaaggcaca gtcgaggctg atcagcgagc tctagcattt aggtgacact
      301 atagaatagg gccctctaga tgcatgctcg agcggccgct tttttttttt tttttttttt
      361 tttttttttt ttatgtgtcc aagtagagat ttgccattca ctctgtttaa taaagtccat
      421 aaagagttga aagccaaatg tttggagatc atggttggat acacatctca attcccacac
      481 acctcccagt tcccacagca ggcccgccga agtcctagga atgtgcctag gcaggcacag
      541 agtggaccca gataacgtca accctgctgg ttccctaagc agaccaccca aagaccccgc
      601 attgatccct gcatagtgtc cattatttct cccctgggac catggaatcc ttgaaaacct
      661 cagcatcctc tggcttaagg gatcccgtat aatgcttcag ttgaacttct tcctgctggg
      721 tggcatgtct atctcgagtg cttgcatccc tcagaagggg aaccagggca aagttcttcc
      781 agtttctccc tgaaactcgg cctgacctca gggagaagct cagggtgcgc ttgacgccct
      841 cacagttgcc tgggtcctcg ttgatgatac ccacattggt gttccaggta gtccagttta
      901 cctcgtccac cctgaaacac caccggtagt catccttgcc gtcaggagtg aaccccacct
      961 gcagcagctt gccagagcgg aaggccttcc tcatgcactt caggaagctc ttctctgtat
     1021 ccaggatggt gatggctctc tgcagcttcc agatgttctt gctctcttgt gcaatcttgt
     1081 tgacggtctc acccatgaga gcaatgagca tgttgagcag aaggatgtag gtgagaatca
     1141 cataggccag taacaggatg atgaagacag ccttgaagtc gtagttctca gtgaactcca
     1201 ggtcgcccat gccgatggtg aacttgaaca gctccagaca tgtggaatac aggctgttgt
     1261 aagagttacc tggcttgcag gcagaccccc ggcacttgtg tggtgtggac tccataggca
     1321 gcccggcaaa ttctggccat tcgtcccagg tcgggatagg gcggatgaac tccataaatg
     1381 caataccttt gacgcgctct ggattgcgct tggcccagtg gaaacccaga gcggagcccc
     1441 agtcgtgaat gaccaggacg acctcttcca gacccagggc ttcgatgaag gcatccatga
     1501 agcggacgtg gtcgtcgaag aaataaccca ggtctggttt gtcggatttg cccataccga
     1561 tcaggtctgg agcaatgcag cgatgggtcg gtgcaacatg cgggatgatg ttgcgccaca
     1621 cgtaggagga ggtcgggtta ccgtgcagga acagcacagg ggtgccatcg cgcggaccaa
     1681 catcgacgta gtgcatgcgc tcgcccagga cttccacata atgggggtcg aatggaaagc
     1741 cagtaccgat ttctgccatg ctgcctcctg tgcctccgct gcctcctgtg cctccgctgc
     1801 ctccagtggt tggctcgccg gaaatctcga gcgtcgacag ccagcgcgcg atctcgctgc
     1861 cgatcaggtc cgggttgtct tcttgcagca gattcagacc cgggccgatg tccacagcct
     1921 tgcagttagg caggcttttg gccaggcgag cggcttcggc cggtgggatc agaacgcctg
     1981 gggtgcccca gaacagcagc ttcgggacag gggactggtg cagccagtcc atgtattctt
     2041 cgaccagcgc gacgatgttc gctggctcac cggcgattgg cagctcgttt gggaagcgcc
     2101 acagtggctc gcggtcaaca ggattcagga acggctcgcg gtaatggtcc atctcgactt
     2161 cagtcagcgg gcggacgaca cccatcggca gcgtaccctc gataaaaacg ttctgatcga
     2221 tgatcagctt gcggccgacg tcggtggtgc ggaaggcctg gaaggtctcg cgggcaaagg
     2281 cagagttatt cttcccatcc tcaatcagtg tcaccacagc tgtggaaaat ccaaacaaga
     2341 acacgaggta gacgaacata aaccggcaca ggtctctgag gatcatcttc tcaatcatga
     2401 cagcatagat gcccatctgc tggaatcctc gggtatagta gagcatgttg gtccagccca
     2461 tggccaggga gaacaccatg gaagccacat actccttgcg ttggctgaag tacagtacca
     2521 cagacaccag catgaacagc gactgtacaa agaaaagtat ctcactgtag ctgtccacaa
     2581 acaaactctt gagggatggt cgcctctgca ggaaatattg aatccctcgg aagaagaagt
     2641 agactcctcc tgacacagac aagatctctc cggtgactcg gaaatagtcc ccaacggtgt
     2701 ttttcagctt ataggggggc aagccttcca caggccgata gtaggcagcc gcggtgaaga
     2761 tgatcatata caagcagtag acgaagaagt tgaagtagaa gatgcgcttg acaaatctgt
     2821 cccacttgtc ctgtaggagt cggttcaagg gttccacgag aagcatgtca tgacggttag
     2881 gggtctcact gctgctgtaa gcgatcacct ccagaaccga gttcttttca caggtgtcaa
     2941 tgcaggacag gtcataaagg gaggagtgca ctggcccata ggcccattcg gtgaacttcc
     3001 tggataggtg tcggcactcg ggttcatgga tctccctctg gagaatgtag gccaagaccc
     3061 cgatcttccc actgctagca gccagagcca gtggcgtgag ccccttcctg ttggtgatct
     3121 cttccagctt cagcgtgggg tggagtttgg cccccaggat caagatctcg ttgtacatgc
     3181 ttgtcacgaa cttggtgttg tcaactgtgt tatctgccac ctccaccagg gcatgaagca
     3241 ccgtgttgcc cactgagtcc cgggcgctga tgtctgcagg ctgccaggag ttctgcagca
     3301 ggaacttcac aatggccagc tggttggtgc acgcagccag ggacaggggc agctcaccaa
     3361 agtagaagcc aggcctccct ttggttttct tgaagaagtc cccgttagcc gcagcctgga
     3421 catctgctcc attctccacc aagagggtca ccagcgtcat gttccgccgt tcaatggcaa
     3481 tgtgcagtgc tgtctggccc ttgtagtagc tgtctgtgta gctggcattg acaaactgct
     3541 tcaggctgtc tgtcttccgg gcaacgtcca ggagcagagc gatggtgtca ttctgcccat
     3601 tgtgcagatt gagcatggct tttagcagac aggtctttcc tgtctctggg tctttgaact
     3661 cgctgtcagt caggcgcttc ttgctcctct gcaggaaggg cagcaggctc tccagctcct
     3721 ggcagttact ctgagccaca gcatcgaaga tgctcctgcg atcatagagc ctcgggggct
     3781 tctcaccagc ggagacggag tcctgggatg acggcctgac actggcaggt ccatccccag
     3841 gcctctggat agttagaaca gagctgacag tgatgatagg gcaggaagcc agcccgcctt
     3901 cctcataagg gcagtccagg ggagaggcct cctccgagtc acccttccca aaaagccggg
     3961 tacgactcct ggtagtgaag atgtggggct tgactggagg tggcttgcag ttagggtctc
     4021 tgtctggagg gtccaggcag gagttctctt ggggtgggga ctcagactcc tctgagtcta
     4081 agctagcccg ttgttccatc ctttccagat cctctgtggc ccaatttgca accagctagg
     4141 tgcaggcaca ccccaaatgg agcaagtgcc ttggagctgc tttccagcac actggcggcc
     4201 gttactagtg gatccgagct cggtaccaag cttgggtctc cctatagtga gtcgtattaa
     4261 tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg cttatataga
     4321 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     4381 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     4441 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     4501 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     4561 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     4621 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     4681 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     4741 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     4801 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     4861 attaataact agtcaataat caatgtcaac gcgtatatct ggcccgtaca tcgcgaagca
     4921 gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg
     4981 ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca
     5041 gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac cataggggat
     5101 cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5161 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5221 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5281 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5341 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5401 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5461 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5521 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5581 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5641 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5701 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5761 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5821 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5881 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5941 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6001 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6061 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6121 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6181 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6241 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6301 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6361 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6421 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6481 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6541 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6601 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6661 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6721 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6781 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6841 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6901 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6961 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7021 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7081 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7141 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7201 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     7261 cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg acggtataca
     7321 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     7381 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     7441 aaacaagttg gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     7501 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     7561 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     7621 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     7681 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     7741 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
     7801 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     7861 acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc
     7921 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     7981 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     8041 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     8101 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     8161 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     8221 cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     8281 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     8341 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     8401 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga tccgaaaatg
     8461 gatatacaag ctcccgggag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     8521 tacttctgga atagctcaga ggcagaggcg gcctcggcct ctgcataaat aaaaaaaatt
     8581 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     8641 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     8701 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
     8761 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
     8821 agaattaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     8881 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     8941 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     9001 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     9061 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     9121 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     9181 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     9241 cgctacaggg cgcgtgggga tacc
//
