LOCUS       sequence-436669-        8176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split intein version of Trojan-Gal4 Expression Module in Phase 1. 
            Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be 
            converted by cassette exchange into other Trojan exons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Diao F, Vasudevan D, Heckscher ES, White BH
  TITLE     Hox gene-specific cellular targeting using split intein Trojan 
            exons.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Apr 23;121(17):e2317083121. doi: 
            10.1073/pnas.2317083121. Epub 2024 Apr 11.
  PUBMED    38602904
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-04-23"; volume: "121(17):e2317083121. 
            doi"; pages: " 10.1073/pnas.2317083121. Epub 2024 Apr 11"
COMMENT     Sequence Label: psiT-GEM(1)
FEATURES             Location/Qualifiers
     source          1..8176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..19
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3..19
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    73..172
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             590..643
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             644..3286
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             3287..3340
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    3577..3610
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          4597..4662
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4792..4812
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5084..5218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5121..5140)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5175..5194
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    5226..5259
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    5260..5359
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     primer_bind     complement(5390..5410)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5390..5408)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5429..5445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5429..5445)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5442..5464)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5453..5469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5477..5483,5484..5501,5502..5507))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5522..5543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5660..5677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5831..6419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5911..5930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6590..7381,7382..7450))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7213..7232
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7451..7555)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7582..8037
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7669..7688)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7879..7900
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(8164..8176,1..10)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgacc caccggtgca
       61 tgcggcggcc gccccaggtc agaagcggtt ttcgggagta gtgccccaac tggggtaacc
      121 tttgagttct ctcagttggg ggcgtagggt cgccgacatg acacaagggg tttctagaag
      181 tcgatccaac atggcgactt gtcccatccc cggcatgttt aaatatacta attattcttg
      241 aactaatttt aatcaaccga tttatctctc ttccgcaggt gaatattgcc tgtcctacga
      301 caccgagatc ctgaccgtgg agtacggctt cctgcccatc ggcaagatcg tggaggagcg
      361 catcgagtgc accgtgtaca ccgtggacaa gaacggcttc gtgtacaccc agcccatcgc
      421 ccagtggcac aaccgcggcg agcaggaggt gttcgagtac tgcctggagg acggctccat
      481 catccgcgcc accaaggacc acaagttcat gaccaccgac ggccagatgc tgcccatcga
      541 cgagatcttc gagcgcggcc tggacctgaa gcaggtggac ggcctgcccg agggccgcgg
      601 cagcctgctg acctgcggcg atgtggagga gaaccccggg cccatgaagc tactgtcttc
      661 tatcgaacaa gcctgcgata tttgccgact taaaaagctc aagtgctcca aagaaaaacc
      721 gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc tactctccca aaaccaaaag
      781 gtctccgctg actagggcac atctgacaga agtggaatca aggctagaaa gactggaaca
      841 gctatttcta ctgatttttc ctcgagaaga ccttgacatg attttgaaaa tggattcttt
      901 acaggatata aaagcattgt taacaggatt atttgtacaa gataatgtga ataaagatgc
      961 cgtcacagat agattggctt cagtggagac tgatatgcct ctaacattga gacagcatag
     1021 aataagtgcg acatcatcat cggaagagag tagtaacaaa ggtcaaagac agttgactgt
     1081 atcgattgac tcggcagctc atcatgataa ctccacaatt ccgttggatt ttatgcccag
     1141 ggatgctctt catggatttg attggtctga agaggatgac atgtcggatg gcttgccctt
     1201 cctgaaaacg gaccccaaca ataatgggtt ctttggcgac ggttctctct tatgtattct
     1261 tcgatctatt ggctttaaac cggaaaatta cacgaactct aacgttaaca ggctcccgac
     1321 catgattacg gatagataca cgttggcttc tagatccaca acatcccgtt tacttcaaag
     1381 ttatctcaat aattttcacc cctactgccc tatcgtgcac tcaccgacgc taatgatgtt
     1441 gtataataac cagattgaaa tcgcgtcgaa ggatcaatgg caaatccttt ttaactgcat
     1501 attagccatt ggagcctggt gtatagaggg ggaatctact gatatagatg ttttttacta
     1561 tcaaaatgct aaatctcatt tgacgagcaa ggtcttcgag tcaggttcca taattttggt
     1621 gacagcccta catcttctgt cgcgatatac acagtggagg cagaaaacaa atactagcta
     1681 taattttcac agcttttcca taagaatggc catatcattg ggcttgaata gggacctccc
     1741 ctcgtccttc agtgatagca gcattctgga acaaagacgc cgaatttggt ggtctgtcta
     1801 ctcttgggag atccaattgt ccctgcttta tggtcgatcc atccagcttt ctcagaatac
     1861 aatctccttc ccttcttctg tcgacgatgt gcagcgtacc acaacaggtc ccaccatata
     1921 tcatggcatc attgaaacag caaggctctt acaagttttc acaaaaatct atgaactaga
     1981 caaaacagta actgcagaaa aaagtcctat atgtgcaaaa aaatgcttga tgatttgtaa
     2041 tgagattgag gaggtttcga gacaggcacc aaagttttta caaatggata tttccaccac
     2101 cgctctaacc aatttgttga aggaacaccc ttggctatcc tttacaagat tcgaactgaa
     2161 gtggaaacag ttgtctctta tcatttatgt attaagagat tttttcacta attttaccca
     2221 gaaaaagtca caactagaac aggatcaaaa tgatcatcaa agttatgaag ttaaacgatg
     2281 ctccatcatg ttaagcgatg cagcacaaag aactgttatg tctgtaagta gctatatgga
     2341 caatcataat gtcaccccat attttgcctg gaattgttct tattacttgt tcaatgcagt
     2401 cctagtaccc ataaagactc tactctcaaa ctcaaaatcg aatgctgaga ataacgagac
     2461 cgcacaatta ttacaacaaa ttaacactgt tctgatgcta ttaaaaaaac tggccacttt
     2521 taaaatccag acttgtgaaa aatacattca agtactggaa gaggtatgtg cgccgtttct
     2581 gttatcacag tgtgcaatcc cattaccgca tatcagttat aacaatagta atggtagcgc
     2641 cattaaaaat atcgtcggtt ctgcaactat cgcccaatac cctactcttc cggaggaaaa
     2701 tgtcaacaat atcagtgtta aatatgtttc tcctggctca gtagggcctt cacctgtgcc
     2761 attgaaatca ggagcaagtt tcagtgatct agtcaagctg ttatctaacc gtccaccctc
     2821 tcgtaactct ccagtgacaa taccaagaag cacaccttcg catcgctcag tcacgccttt
     2881 tctagggcaa cagcaacagc tgcaatcatt agtgccactg accccgtctg ctttgtttgg
     2941 tggcgccaat tttaatcaaa gtgggaatat cgctgatagc tcattgtcct tcactttcac
     3001 taacagtagc aacggtccga acctcataac aactcaaaca aattctcaag cgctttcaca
     3061 accaattgcc tcctctaacg ttcatgataa cttcatgaat aatgaaatca cggctagtaa
     3121 aattgatgat ggtaataatt caaaaccact gtcacctggt tggacggacc aaactgcgta
     3181 taacgcgttt ggaatcacta cagggatgtt taataccact acaatggatg atgtatataa
     3241 ctatctattc gatgatgaag ataccccacc aaacccaaaa aaagaggagg gccgcggcag
     3301 cctgctgacc tgcggcgatg tggaggagaa ccccgggccc gtgaagatca tctcccgcaa
     3361 gtccctgggc acccagaacg tgtacgacat cggcgtggag aaggaccaca acttcctgct
     3421 gaagaacggc ctggtggcct ccaactgctt caacggtaag ttattgaaca atggcatcaa
     3481 atgccttcat catcactacc ctttagccct taagacccca caatgacctt acccactcag
     3541 agaaaaaagt aaatatgaaa gcccatttga acttctgaag ttcctattct ctagaaagta
     3601 taggaacttc tcctagggga tctaattcaa ttagagacta attcaattag agctaattca
     3661 attaggatcc aagcttatcg atttcgaacc ctcgaccgcc ggagtataaa tagaggcgct
     3721 tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag
     3781 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat cggactagag ccggtcgcca
     3841 ccatgaggtc ttccaagaat gttatcaagg agttcatgag gtttaaggtt cgcatggaag
     3901 gaacggtcaa tgggcacgag tttgaaatag aaggcgaagg agaggggagg ccatacgaag
     3961 gccacaatac cgtaaagctt aaggtaacca aggggggacc tttgccattt gcttgggata
     4021 ttttgtcacc acaatttcag tatggaagca aggtatatgt caagcaccct gccgacatac
     4081 cagactataa aaagctgtca tttcctgaag gatttaaatg ggaaagggtc atgaactttg
     4141 aagacggtgg cgtcgttact gtaacccagg attccagttt gcaggatggc tgtttcatct
     4201 acaaggtcaa gttcattggc gtgaactttc cttccgatgg acctgttatg caaaagaaga
     4261 caatgggctg ggaagccagc actgagcgtt tgtatcctcg tgatggcgtg ttgaaaggag
     4321 agattcataa ggctctgaag ctgaaagacg gtggtcatta cctagttgag ttcaaaagta
     4381 tttacatggc aaagaagcct gtgcagctac cagggtacta ctatgttgac tccaaactgg
     4441 atataacaag ccacaacgaa gactatacaa tcgttgagca gtatgaaaga accgagggac
     4501 gccaccatct gttcctttag gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     4561 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     4621 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     4681 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     4741 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     4801 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     4861 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
     4921 gcactgctat acaagaaaat tatggaaaaa tatttgatgt atagtgcctt gactagagat
     4981 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     5041 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     5101 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5161 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcgc
     5221 tagcagaagt tcctattctc tagaaagtat aggaacttca accccttgtg tcatgtcggc
     5281 gaccctacgc ccccaactga gagaactcaa aggttacccc agttggggca ctactcccga
     5341 aaaccgcttc tgacctgggg gcgcgccggt accactagtg gggaggttcc ctttagtgag
     5401 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5461 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5521 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5581 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5641 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5701 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5761 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5821 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5881 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5941 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6001 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6061 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6121 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6181 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6241 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     6301 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6361 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6421 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6481 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6541 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6601 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6661 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6721 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6781 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6841 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6901 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6961 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7021 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7081 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7141 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7201 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7261 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7321 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7381 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7441 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7501 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7561 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     7621 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     7681 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     7741 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     7801 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7861 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     7921 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7981 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc
     8041 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     8101 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     8161 tttcccagtc acgacg
//
