LOCUS       sequence-43668-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE1c-RFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    203..219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(227..233,234..250,251..256))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 cagcggacca cctttggtaa ctttcagttt agcggtctgg gtaccttcgt acggacgacc
       61 ttcaccttca ccttcgattt cgaactcgtg accgttaacg gaaccttcca tacgaacttt
      121 gaaacgcatg aactctttga taacgtcttc gctactcgcc atatgtatat ctccttctta
      181 aaagatcttt tgaattctga aattgttatc cgctcacaat tccacacatt atacgagccg
      241 gatgattaat tgtcaacagc tcatttcaga atatttgcca gaaccgttat gatgtcggcg
      301 caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattatg cagtgattta
      361 cgacctgcac agccatacca cagcttccga tggctgcctg acgccagaag cattggtgca
      421 ccgtgcagtc gatgataagc tgtcaaacca gatcaattcg cgctaactta cattaattgc
      481 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      541 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
      601 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
      661 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
      721 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
      781 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
      841 ccagcatcgc agtgggaacg
//
