LOCUS       sequence-43669-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE1k-RFP
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      150..221
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      237..264
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(502..521)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
       61 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaagga
      121 tccaaactcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact
      181 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      241 caccttcggg tgggcctttc tgcgtttata cctagggcgt tcggctgcgg cgagcggtat
      301 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      361 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      421 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      481 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      541 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      601 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      661 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      721 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      781 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagt
//
