LOCUS       sequence-436690-        7060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Zhang SQ, Tsiaras WG, Araki T, Wen G, Minichiello L, Klein R, Neel 
            BG
  TITLE     Receptor-specific regulation of phosphatidylinositol 3'-kinase 
            activation by the protein tyrosine phosphatase Shp2.
  JOURNAL   Mol Cell Biol 2002 Jun;22(12):4062-72.
  PUBMED    12024020
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "2002-06"; volume: "22(12)"; pages: "4062-72"
COMMENT     Sequence Label: pBABE-puro SHP2 E76A
FEATURES             Location/Qualifiers
     source          1..7060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1443..1463)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1448..1777
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1628..1763
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1787..2386
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1787..1806)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2283..2303
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2607..3076
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3206..3223)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3377..3965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3457..3476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4125..4916,4917..4985))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4748..4767
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4986..5090)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5121..5590
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5655..5854
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             6055..6471
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6401..6423
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6439..6455
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 ttggtggagg agaacggttt gattctttga cagatcttgt ggaacattat aagaagaatc
       61 ctatggtgga aacattgggt acagtactac aactcaagca gccccttaac acgactcgta
      121 taaatgctgc tgaaatagaa agcagagttc gagaactaag caaattagct gagaccacag
      181 ataaagtcaa acaaggcttt tgggaagaat ttgagacact acaacaacag gagtgcaaac
      241 ttctctacag ccgaaaagag ggtcaaaggc aagaaaacaa aaacaaaaat agatataaaa
      301 acatcctgcc ctttgatcat accagggttg tcctacacga tggtgatccc aatgagcctg
      361 tttcagatta catcaatgca aatatcatca tgcctgaatt tgaaaccaag tgcaacaatt
      421 caaagcccaa aaagagttac attgccacac aaggctgcct gcaaaacacg gtgaatgact
      481 tttggcggat ggtgttccaa gaaaactccc gagtgattgt catgacaacg aaagaagtgg
      541 agagaggaaa gagtaaatgt gtcaaatact ggcctgatga gtatgctcta aaagaatatg
      601 gcgtcatgcg tgttaggaac gtcaaagaaa gcgccgctca tgactatacg ctaagagaac
      661 ttaaactttc aaaggttgga caagggaata cggagagaac ggtctggcaa taccactttc
      721 ggacctggcc ggaccacggc gtgcccagcg accctggggg cgtgctggac ttcctggagg
      781 aggtgcacca taagcaggag agcatcatgg atgcagggcc ggtcgtggtg cactgcagtg
      841 ctggaattgg ccggacaggg acgttcattg tgattgatat tcttattgac atcatcagag
      901 agaaaggtgt tgactgcgat attgacgttc ccaaaaccat ccagatggtg cggtctcaga
      961 ggtcagggat ggtccagaca gaagcacagt accgatttat ctatatggcg gtccagcatt
     1021 atattgaaac actacagcgc aggattgaag aagagcagaa aagcaagagg aaagggcacg
     1081 aatatacaaa tattaagtat tctctagcgg accagacgag tggagatcag agccctctcc
     1141 cgccttgtac tccaacgcca ccctgtgcag aaatgagaga agacagtgct agagtctatg
     1201 aaaacgtggg cctgatgcaa cagcagaaaa gtttcagatg agaaaacctg ccaaaacttc
     1261 agcacagaaa tagatgtgga ctttcaccct ctccctaaaa agatcaagaa cagacgcaag
     1321 aaagtttatg tgaagacaga atttggattt ggaaggcttg caatgtggtt gactaccttt
     1381 tgataagcaa aatttgaaac catttaaaga ccggaattcg ccagcacagt ggtcgaccct
     1441 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     1501 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     1561 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     1621 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     1681 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     1741 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttaccatga ccgagtacaa
     1801 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     1861 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     1921 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     1981 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     2041 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     2101 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     2161 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     2221 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     2281 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     2341 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     2401 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgataaaat aaaagatttt
     2461 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     2521 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag
     2581 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
     2641 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
     2701 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     2761 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     2821 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     2881 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
     2941 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     3001 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     3061 cagcgggggt ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta
     3121 tttacattaa atggccatag ttgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3181 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3241 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3301 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3361 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3421 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3481 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3541 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3601 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3661 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3721 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3781 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3841 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3901 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3961 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4021 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgcggc
     4081 cgcaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4141 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4201 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4261 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4321 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4381 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4441 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4501 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4561 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4621 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4681 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4741 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4801 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4861 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4921 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4981 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5041 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5101 cgaaaagtgc cacctgcagc ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     5161 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     5221 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     5281 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     5341 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     5401 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     5461 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     5521 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     5581 gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc
     5641 caccaccggg aggcaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct
     5701 atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg tatctggcgg
     5761 acccgtggtg gaactgacga gttctgaaca cccggccgca accctgggag acgtcccagg
     5821 gactttgggg gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc
     5881 ccgtcaggat atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct
     5941 gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc
     6001 atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag
     6061 actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct
     6121 cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg
     6181 ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag
     6241 gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc
     6301 gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct
     6361 aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg
     6421 accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc cggccggatc
     6481 ccagtgtggt ggtacgtagg aattccgggc gggaggaaca tgacatcgcg gagatggttt
     6541 cacccaaata tcactggtgt ggaggcagaa aacctactgt tgacaagagg agttgatggc
     6601 agttttttgg caaggcctag taaaagtaac cctggagact tcacactttc cgttagaaga
     6661 aatggagctg tcacccacat caagattcag aacactggtg attactatga cctgtatgga
     6721 ggggagaaat ttgccacttt ggctgcgttg gtccagtatt acatggaaca tcacgggcaa
     6781 ttaaaagaga agaatggaga tgtcattgag cttaaatatc ctctgaactg tgcagatcct
     6841 acctctgaaa ggtggtttca tggacatctc tctgggaaag aagcagagaa attattaact
     6901 gaaaaaggaa aacatggtag ttttcttgta cgagagagcc agagccaccc tggagatttt
     6961 gttctttctg tgcgcactgg tgatgacaaa ggggagagca atgacggcaa gtctaaagtg
     7021 acccatgtta tgattcgctg tcaggaactg aaatacgacg
//
