LOCUS       sequence-436691-       13398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Cacchiarelli D, Trapnell C, Ziller MJ, Soumillon M, Cesana M, Karnik
            R, Donaghey J, Smith ZD, Ratanasirintrawoot S, Zhang X, Ho Sui SJ, 
            Wu Z, Akopian V, Gifford CA, Doench J, Rinn JL, Daley GQ, Meissner 
            A, Lander ES, Mikkelsen TS
  TITLE     Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of
            Induced Pluripotency.
  JOURNAL   Cell. 2015 Jul 16;162(2):412-24. doi: 10.1016/j.cell.2015.06.016.
  PUBMED    26186193
REFERENCE   2  (bases 1 to 13398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.06"; journalName: "Cell"; date: "2015-07-16- 
            16"; volume: "162"; issue: "2"; pages: "412-24"
COMMENT     Sequence Label: FUW-tetO-hOKMS
FEATURES             Location/Qualifiers
     source          1..13398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2724
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2721..2745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3957..4010
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5430..5486
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5487..6803
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             6813..6872
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     misc_feature    7859..8447
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7912..7932)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8330..8341)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8450..8466)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8451..8467)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8972..9152
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9178..9195)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9184..9408
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9454..9882
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9541..9560)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9751..9772
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9891..9911)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9896..10225
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10076..10211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10138..10157
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10273..10320
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10339..10713
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10843..10964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10880..10899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10934..10953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11013..11029)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11013..11029)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11026..11048)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11037..11053
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11061..11067,11068..11085,11086..11091))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11106..11127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11244..11261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11415..12003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11495..11514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12174..12965,12966..13034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12797..12816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13035..13139)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13214..13233)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg
     2701 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2761 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa
     2821 ttcgttgacc gaatcaccga cctctctccc cagggggatc caccggtatg gcgggacacc
     2881 tggcttcgga tttcgccttc tcgccccctc caggtggtgg aggtgatggg ccaggggggc
     2941 cggagccggg ctgggttgat cctcggacct ggctaagctt ccaaggccct cctggagggc
     3001 caggaatcgg gccgggggtt gggccaggct ctgaggtgtg ggggattccc ccatgccccc
     3061 cgccgtatga gttctgtggg gggatggcgt actgtgggcc ccaggttgga gtggggctag
     3121 tgccccaagg cggcttggag acctctcagc ctgagggtga agcaggagtc ggggtggaga
     3181 gcaactccga tggggcctcc ccggagccct gcaccgtcac ccctggtgcc gtgaagctgg
     3241 agaaggagaa gctggagcaa aacccggagg agtcccagga catcaaagct ctgcagaaag
     3301 aactcgagca atttgccaag ctcctgaagc agaagaggat caccctggga tatacacagg
     3361 ccgatgtggg gctcaccctg ggggttctat ttgggaaggt attcagccaa acgaccatct
     3421 gccgctttga ggctctgcag cttagcttca agaacatgtg taagctgcgg cccttgctgc
     3481 agaagtgggt ggaggaagct gacaacaatg aaaatcttca ggagatatgc aaagcagaaa
     3541 ccctcgtgca ggcccgaaag agaaagcgaa ccagtatcga gaaccgagtg agaggcaacc
     3601 tggagaattt gttcctgcag tgcccgaaac ccacactgca gcagatcagc cacatcgccc
     3661 agcagcttgg gctcgagaag gatgtggtcc gagtgtggtt ctgtaaccgg cgccagaagg
     3721 gcaagcgatc aagcagcgac tatgcacaac gagaggattt tgaggctgct gggtctcctt
     3781 tctcaggggg accagtgtcc tttcctctgg ccccagggcc ccattttggt accccaggct
     3841 atgggagccc tcacttcact gcactgtact cctcggtccc tttccctgag ggggaagcct
     3901 ttccccctgt ctctgtcacc actctgggct ctcccatgca ttcaaacgga tctggagagg
     3961 gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggcccc atggctgtca
     4021 gcgacgcgct gctcccatct ttctccacgt tcgcgtctgg cccggcggga agggagaaga
     4081 cactgcgtca agcaggtgcc ccgaataacc gctggcggga ggagctctcc cacatgaagc
     4141 gacttccccc agtgcttccc ggccgcccct atgacctggc ggcggcgacc gtggccacag
     4201 acctggagag cggcggagcc ggtgcggctt gcggcggtag caacctggcg cccctacctc
     4261 ggagagagac cgaggagttc aacgatctcc tggacctgga ctttattctc tccaattcgc
     4321 tgacccatcc tccggagtca gtggccgcca ccgtgtcctc gtcagcgtca gcctcctctt
     4381 cgtcgtcgcc gtcgagcagc ggccctgcca gcgcgccctc cacctgcagc ttcacctatc
     4441 cgatccgggc cgggaacgac ccgggcgtgg cgccgggcgg cacgggcgga ggcctcctct
     4501 atggcaggga gtccgctccc cctccgacgg ctcccttcaa cctggcggac atcaacgacg
     4561 tgagcccctc gggcggcttc gtggccgagc tcctgcggcc agaattggac ccggtgtaca
     4621 ttccgccgca gcagccgcag ccgccaggtg gcgggctgat gggcaagttc gtgctgaagg
     4681 cgtcgctgag cgcccctggc agcgagtacg gcagcccgtc ggtcatcagc gtcagcaaag
     4741 gcagccctga cggcagccac ccggtggtgg tggcgcccta caacggcggg ccgccgcgca
     4801 cgtgccccaa gatcaagcag gaggcggtct cttcgtgcac ccacttgggc gctggacccc
     4861 ctctcagcaa tggccaccgg ccggctgcac acgacttccc cctggggcgg cagctcccca
     4921 gcaggactac cccgaccctg ggtcttgagg aagtgctgag cagcagggac tgtcaccctg
     4981 ccctgccgct tcctcccggc ttccatcccc acccggggcc caattaccca tccttcctgc
     5041 ccgatcagat gcagccgcaa gtcccgccgc tccattacca agagctcatg ccacccggtt
     5101 cctgcatgcc agaggagccc aagccaaaga ggggaagacg atcgtggccc cggaaaagga
     5161 ccgccaccca cacttgtgat tacgcgggct gcggcaaaac ctacacaaag agttcccatc
     5221 tcaaggcaca cctgcgaacc cacacaggtg agaaacctta ccactgtgac tgggacggct
     5281 gtggatggaa attcgcccgc tcagatgaac tgaccaggca ctaccgtaaa cacacggggc
     5341 accgcccgtt ccagtgccaa aaatgcgacc gagcattttc caggtcggac cacctcgcct
     5401 tacacatgaa gaggcatttt ggatctggag caacaaactt ctcactactc aaacaagcag
     5461 gtgacgtgga ggagaatccc ggccctatgc ccctcaacgt tagcttcacc aacaggaact
     5521 atgacctcga ctacgactcg gtgcagccgt atttctactg cgacgaggag gagaacttct
     5581 accagcagca gcagcagagc gagctgcagc ccccggcgcc cagcgaggat atctggaaga
     5641 aattcgagct gctgcccacc ccgcccctgt cccctagccg ccgctccggg ctctgctcgc
     5701 cctcctacgt tgcggtcaca cccttctccc ttcggggaga caacgacggc ggtggcggga
     5761 gcttctccac ggccgaccag ctggagatgg tgaccgagct gctgggagga gacatggtga
     5821 accagagttt catctgcgac ccggacgacg agaccttcat caaaaacatc atcatccagg
     5881 actgtatgtg gagcggcttc tcggccgccg ccaagctcgt ctcagagaag ctggcctcct
     5941 accaggctgc gcgcaaagac agcggcagcc cgaaccccgc ccgcggccac agcgtctgct
     6001 ccacctccag cttgtacctg caggatctga gcgccgccgc ctcagagtgc atcgacccct
     6061 cggtggtctt cccctaccct ctcaacgaca gcagctcgcc caagtcctgc gcctcgcaag
     6121 actccagcgc cttctctccg tcctcggatt ctctgctctc ctcgacggag tcctccccgc
     6181 agggcagccc cgagcccctg gtgctccatg aggagacacc gcccaccacc agcagcgact
     6241 ctgaggagga acaagaagat gaggaagaaa tcgatgttgt ttctgtggaa aagaggcagg
     6301 ctcctggcaa aaggtcagag tctggatcac cttctgctgg aggccacagc aaacctcctc
     6361 acagcccact ggtcctcaag aggtgccacg tctccacaca tcagcacaac tacgcagcgc
     6421 ctccctccac tcggaaggac tatcctgctg ccaagagggt caagttggac agtgtcagag
     6481 tcctgagaca gatcagcaac aaccgaaaat gcaccagccc caggtcctcg gacaccgagg
     6541 agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca gaggaggaac gagctaaaac
     6601 ggagcttttt tgccctgcgt gaccagatcc cggagttgga aaacaatgaa aaggccccca
     6661 aggtagttat ccttaaaaaa gccacagcat acatcctgtc cgtccaagca gaggagcaaa
     6721 agctcatttc tgaagaggac ttgttgcgga aacgacgaga acagttgaaa cacaaacttg
     6781 aacagctacg gaactcttgt gcgggatctg gacaatgtac taactacgct ttgttgaaac
     6841 tcgctggcga tgttgaaagt aaccccggtc ccatgtacaa catgatggag acggagctga
     6901 agccgccggg cccgcagcaa acttcggggg gcggcggcgg caactccacc gcggcggcgg
     6961 ccggcggcaa ccagaaaaac agcccggacc gcgtcaagcg gcccatgaat gccttcatgg
     7021 tgtggtcccg cgggcagcgg cgcaagatgg cccaggagaa ccccaagatg cacaactcgg
     7081 agatcagcaa gcgcctgggc gccgagtgga aacttttgtc ggagacggag aagcggccgt
     7141 tcatcgacga ggctaagcgg ctgcgagcgc tgcacatgaa ggagcacccg gattataaat
     7201 accggccccg gcggaaaacc aagacgctca tgaagaagga taagtacacg ctgcccggcg
     7261 ggctgctggc ccccggcggc aatagcatgg cgagcggggt cggggtgggc gccggcctgg
     7321 gcgcgggcgt gaaccagcgc atggacagtt acgcgcacat gaacggctgg agcaacggca
     7381 gctacagcat gatgcaggac cagctgggct acccgcagca cccgggcctc aatgcgcacg
     7441 gcgcagcgca gatgcagccc atgcaccgct acgacgtgag cgccctgcag tacaactcca
     7501 tgaccagctc gcagacctac atgaacggct cgcccaccta cagcatgtcc tactcgcagc
     7561 agggcacccc tggcatggct cttggctcca tgggttcggt ggtcaagtcc gaggccagct
     7621 ccagcccccc tgtggttacc tcttcctccc actccagggc gccctgccag gccggggacc
     7681 tccgggacat gatcagcatg tatctccccg gcgccgaggt gccggaaccc gccgccccca
     7741 gcagacttca catgtcccag cactaccaga gcggcccggt gcccggcacg gccattaacg
     7801 gcacactgcc cctctcacac atgtgagtcg acaagaattc gatatcaagc ttatcgataa
     7861 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7921 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7981 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     8041 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     8101 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     8161 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     8221 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     8281 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     8341 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     8401 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     8461 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     8521 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     8581 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     8641 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     8701 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     8761 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     8821 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     8881 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     8941 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     9001 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9061 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9121 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     9181 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     9241 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     9301 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     9361 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     9421 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     9481 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     9541 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9601 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9661 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     9721 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     9781 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     9841 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     9901 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9961 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    10021 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
    10081 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    10141 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
    10201 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
    10261 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
    10321 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
    10381 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
    10441 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
    10501 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
    10561 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
    10621 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
    10681 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
    10741 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
    10801 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
    10861 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    10921 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
    10981 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
    11041 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
    11101 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    11161 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    11221 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    11281 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    11341 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    11401 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    11461 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    11521 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    11581 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    11641 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    11701 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    11761 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    11821 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    11881 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    11941 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    12001 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    12061 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    12121 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    12181 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    12241 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    12301 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    12361 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    12421 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    12481 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    12541 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    12601 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    12661 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    12721 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    12781 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    12841 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    12901 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    12961 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    13021 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    13081 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    13141 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
    13201 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
    13261 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
    13321 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
    13381 atgtacgggc cagatata
//
