LOCUS       sequence-436699-        5999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5999)
  TITLE     SGC-2024-Q1
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: avi-eTMPRSS11D S368A
FEATURES             Location/Qualifiers
     source          1..5999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(86..105)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     295..316
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        479..583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(584..652,653..1444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(802..821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1615..2203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2104..2123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2357..2374
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2508..2732
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2799..3332)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3502..3507,3508..3524,3525..3530))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3882..3973
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3898..3915
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     4029..4091
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             4107..4124
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4137..4181
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             4203..4223
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    5486..5620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5523..5542)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5577..5596
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5649..5814
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      join(5998..5999,1..453)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
       61 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      121 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      181 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
      241 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt aatagtggac
      301 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
      361 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
      421 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
      481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1081 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1141 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1201 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1261 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1321 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1381 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1441 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1741 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     1801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2221 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2281 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2341 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     2401 ttttctcctt acgcatctgt gcggtatttc acaccgcata gaccagccgc gtaacctggc
     2461 aaaatcggtt acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa
     2521 taaagtctta aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca
     2581 gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt
     2641 tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg
     2701 gcgtggccaa gggcatggta aagactatat tcgcggcgtt gtgacaattt accgaacaac
     2761 tccgcggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg
     2821 atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga
     2881 tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc
     2941 ttgagcagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg
     3001 agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg
     3061 agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg
     3121 agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg
     3181 aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc
     3241 ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc
     3301 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
     3361 tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa
     3421 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag
     3481 cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt
     3541 gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag
     3601 gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca
     3661 ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc ctggcttcag
     3721 gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg
     3781 gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga ctctagctat
     3841 agttctagtg gttggctacg tatactccgg aatattaata gatcatggag ataattaaaa
     3901 tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa
     3961 aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg gatctcggtc
     4021 cgaaaaccat gaaattctta gtcaacgttg cccttgtttt tatggtcgta tacatttctt
     4081 acatctatgc ggccgctccg gaaatgcatc atcaccatca ccatgaattc atgagcggcc
     4141 tgaacgatat ttttgaagcg cagaaaattg aatggcatga aggcagcgct ggaggttcag
     4201 gtgaaaactt gtatttccag ggcgatcaaa aatcttactt ttataggagc agttttcaac
     4261 tcctaaatgt tgaatataat agtcagttaa attcaccagc tacacaggaa tacaggactt
     4321 tgagtggaag aattgaatct ctgattacta aaacattcaa agaatcaaat ttaagaaatc
     4381 agttcatcag agctcatgtt gccaaactga ggcaagatgg tagtggtgtg agagcggatg
     4441 ttgtcatgaa atttcaattc actagaaata acaatggagc atcaatgaaa agcagaattg
     4501 agtctgtttt acgacaaatg ctgaataact ctggaaacct ggaaataaac ccttcaactg
     4561 agataacatc acttactgac caggctgcag caaattggct tattaatgaa tgtggggccg
     4621 gtccagacct aataacattg tctgagcaga gaatccttgg aggcactgag gctgaggagg
     4681 gaagctggcc gtggcaagtc agtctgcggc tcaataatgc ccaccactgt ggaggcagcc
     4741 tgatcaataa catgtggatc ctgacagcag ctcactgctt cagaagcaac tctaatcctc
     4801 gtgactggat tgccacgtct ggtatttcca caacatttcc taaactaaga atgagagtaa
     4861 gaaatatttt aattcataac aattataaat ctgcaactca tgaaaatgac attgcacttg
     4921 tgagacttga gaacagtgtc acctttacca aagatatcca tagtgtgtgt ctcccagctg
     4981 ctacccagaa tattccacct ggctctactg cttatgtaac aggatggggc gctcaagaat
     5041 atgctggcca cacagttcca gagctaaggc aaggacaggt cagaataata agtaatgatg
     5101 tatgtaatgc accacatagt tataatggag ccatcttgtc tggaatgctg tgtgctggag
     5161 tacctcaagg tggagtggac gcatgtcagg gtgacgctgg tggcccacta gtacaagaag
     5221 actcacggcg gctttggttt attgtgggga tagtaagctg gggagatcag tgtggcctgc
     5281 cggataagcc aggagtgtat actcgagtga cagcctacct tgactggatt aggcaacaaa
     5341 ctgggatctg atgacgaagc ttgtcgagaa gtactagagg atcataatca gccataccac
     5401 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     5461 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     5521 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5581 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactga tatcgcctag
     5641 gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg
     5701 tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc
     5761 cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg
     5821 catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt gacaaaccgt
     5881 catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat
     5941 taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc gaatgggac
//
