LOCUS       sequence-43670-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE1k-RFP
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    193..209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(217..223,224..240,241..246))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 cctttggtaa ctttcagttt agcggtctgg gtaccttcgt acggacgacc ttcaccttca
       61 ccttcgattt cgaactcgtg accgttaacg gaaccttcca tacgaacttt gaaacgcatg
      121 aactctttga taacgtcttc gctactcgcc atatgtatat ctccttctta aaagatcttt
      181 tgaattctga aattgttatc cgctcacaat tccacacatt atacgagccg gatgattaat
      241 tgtcaacagc tcatttcaga atatttgcca gaaccgttat gatgtcggcg caaaaaacat
      301 tatccagaac gggagtgcgc cttgagcgac acgaattatg cagtgattta cgacctgcac
      361 agccatacca cagcttccga tggctgcctg acgccagaag cattggtgca ccgtgcagtc
      421 gatgataagc tgtcaaacca gatcaattcg cgctaactta cattaattgc gttgcgctca
      481 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      541 gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac
      601 gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac
      661 gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca
      721 tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc
      781 ggactcggta atggcgcgca ttgcgcccag cgccatc
//
