LOCUS       sequence-436700-        5927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5927)
  TITLE     SGC-2024-Q1
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 8VIS
FEATURES             Location/Qualifiers
     source          1..5927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(93..112)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     302..323
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        486..590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(591..659,660..1451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(809..828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1622..2210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2111..2130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2364..2381
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2515..2739
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2806..3339)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3528..3533,3534..3550,3551..3556))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3908..3999
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3924..3941
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     4054..4116
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             4537..4551
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             5260..5283
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     polyA_signal    5421..5555
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5458..5477)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5512..5531
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5584..5749
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
       61 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      121 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      181 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      241 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttcttaat
      301 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
      361 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
      421 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
      481 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      541 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      601 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      661 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      721 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      781 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      841 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      901 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
      961 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1021 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1081 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1141 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1201 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1261 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1321 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1381 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1441 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1501 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1561 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1621 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1681 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1741 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1801 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1861 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1921 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1981 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2041 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2101 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2161 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2221 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2281 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2341 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     2401 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagac cagccgcgta
     2461 acctggcaaa atcggttacg gttgagtaat aaatggatgc cctgcgtaag cgggtgtggg
     2521 cggacaataa agtcttaaac tgaacaaaat agatctaaac tatgacaata aagtcttaaa
     2581 ctagacagaa tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga
     2641 cttttgttat ggctaaagca aactcttcat tttctgaagt gcaaattgcc cgtcgtatta
     2701 aagaggggcg tggccaaggg catggtaaag actatattcg cggcgttgtg acaatttacc
     2761 gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact
     2821 tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac
     2881 tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc
     2941 ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga
     3001 gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta
     3061 agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt
     3121 actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac
     3181 gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg
     3241 gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg
     3301 actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc cataacatca
     3361 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa
     3421 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
     3481 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg
     3541 aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg
     3601 caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg
     3661 tttcggtctc cacgcatcgt caggcattgg cggccttgct gttcttctac ggcaaggtgc
     3721 tgtgcacgga tctgccctgg cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc
     3781 cggtggtgct gaccccggat gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc
     3841 gtttgttcgc ccaggactct agctatagtt ctagtggttg gctacgtata ctccggaata
     3901 ttaatagatc atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac
     3961 tgttttcgta acagttttgt aataaaaaaa cctataaata ttccggatta ttcataccgt
     4021 cccaccatcg ggcgcggatc tcggtccgaa accatgaaat tcttagtcaa cgttgccctt
     4081 gtttttatgg tcgtatacat ttcttacatc tatgcggccg ctgatcaaaa atcttacttt
     4141 tataggagca gttttcaact cctaaatgtt gaatataata gtcagttaaa ttcaccagct
     4201 acacaggaat acaggacttt gagtggaaga attgaatctc tgattactaa aacattcaaa
     4261 gaatcaaatt taagaaatca gttcatcaga gctcatgttg ccaaactgag gcaagatggt
     4321 agtggtgtga gagcggatgt tgtcatgaaa tttcaattca ctagaaataa caatggagca
     4381 tcaatgaaaa gcagaattga gtctgtttta cgacaaatgc tgaataactc tggaaacctg
     4441 gaaataaacc cttcaactga gataacatca cttactgacc aggctgcagc aaattggctt
     4501 attaatgaat gtggggccgg tccagaccta ataacagacg acgacgacaa gatccttgga
     4561 ggcactgagg ctgaggaggg aagctggccg tggcaagtca gtctgcggct caataatgcc
     4621 caccactgtg gaggcagcct gatcaataac atgtggatcc tgacagcagc tcactgcttc
     4681 agaagcaact ctaatcctcg tgactggatt gccacgtctg gtatttccac aacatttcct
     4741 aaactaagaa tgagagtaag aaatatttta attcataaca attataaatc tgcaactcat
     4801 gaaaatgaca ttgcacttgt gagacttgag aacagtgtca cctttaccaa agatatccat
     4861 agtgtgtgtc tcccagctgc tacccagaat attccacctg gctctactgc ttatgtaaca
     4921 ggatggggcg ctcaagaata tgctggccac acagttccag agctaaggca aggacaggtc
     4981 agaataataa gtaatgatgt atgtaatgca ccacatagtt ataatggagc catcttgtct
     5041 ggaatgctgt gtgctggagt acctcaaggt ggagtggacg catgtcaggg tgactctggt
     5101 ggcccactag tacaagaaga ctcacggcgg ctttggttta ttgtggggat agtaagctgg
     5161 ggagatcagt gtggcctgcc ggataagcca ggagtgtata ctcgagtgac agcctacctt
     5221 gactggatta ggcaacaaac tgggatcgaa ttcgtcgagc accaccacca ccaccaccac
     5281 cactaatagc caagcttgtc gagaagtact agaggatcat aatcagccat accacatttg
     5341 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     5401 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     5461 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5521 ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag cctaggagat
     5581 ccgaaccaga taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta
     5641 gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa
     5701 aaactccatt tccacccctc ccagttccca actattttgt ccgcccacag cggggcattt
     5761 ttcttcctgt tatgttttta atcaaacatc ctgccaactc catgtgacaa accgtcatct
     5821 tcggctactt tttctctgtc acagaatgaa aatttttctg tcatctcttc gttattaatg
     5881 tttgtaattg actgaatatc aacgcttatt tgcagcctga atggcga
//
