LOCUS       sequence-436702-        5837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5837)
  TITLE     SGC-2024-Q1
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 8V04
FEATURES             Location/Qualifiers
     source          1..5837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(78..97)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     287..308
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        471..575
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(576..644,645..1436)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(794..813)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1607..2195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2096..2115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2349..2366
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2500..2724
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2791..3324)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3513..3518,3519..3535,3536..3541))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3893..3984
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3909..3926
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     4039..4101
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             4417..4431
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             5155..5178
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     polyA_signal    5316..5450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5353..5372)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5407..5426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5479..5644
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      join(5828..5837,1..445)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
       61 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      121 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
      181 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
      241 atagacggtt tttcgccctt tgacgttgga gtccacgttc ttaatagtgg actcttgttc
      301 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
      361 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
      421 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
      481 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      541 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      601 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      661 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      721 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      781 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      841 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      901 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      961 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1021 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1081 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1141 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1201 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1261 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1321 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1381 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1441 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1501 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1561 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1621 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1681 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1741 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1801 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1861 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1921 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1981 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2041 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2101 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2161 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2221 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2281 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2341 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     2401 ttacgcatct gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg gcaaaatcgg
     2461 ttacggttga gtaataaatg gatgccctgc gtaagcgggt gtgggcggac aataaagtct
     2521 taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga cagaatagtt
     2581 gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt gttatggcta
     2641 aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc
     2701 aagggcatgg taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc
     2761 cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa
     2821 gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc
     2881 gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag
     2941 attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata
     3001 gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc
     3061 aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt
     3121 cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg
     3181 accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt
     3241 gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt
     3301 aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
     3361 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac
     3421 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
     3481 agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca
     3541 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca
     3601 gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc
     3661 atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc
     3721 cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc
     3781 cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg
     3841 actctagcta tagttctagt ggttggctac gtatactccg gaatattaat agatcatgga
     3901 gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt
     3961 tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc
     4021 ggatctcggt ccgaaaccat gaaattctta gtcaacgttg cccttgtttt tatggtcgta
     4081 tacatttctt acatctatgc ggccgcttgt gttcgcctct acggaccaaa cttcatcctt
     4141 caggtgtact catctcagag gaagtcctgg caccctgtgt gccaagacga ctggaacgag
     4201 aactacgggc gggcggcctg cagggacatg ggctataaga ataattttta ctctagccaa
     4261 ggaatagtgg atgacagcgg atccaccagc tttatgaaac tgaacacaag tgccggcaat
     4321 gtcgatatct ataaaaaact gtaccacagt gatgcctgtt cttcaaaagc agtggtttct
     4381 ttacgctgta tagcctgcgg ggtcaacttg aactcagacg acgacgacaa gatcgtgggc
     4441 ggcgagagcg cgctcccggg ggcctggccc tggcaggtca gcctgcacgt ccagaacgtc
     4501 cacgtgtgcg gaggctccat catcaccccc gagtggatcg tgacagccgc ccactgcgtg
     4561 gaaaaacctc ttaacaatcc atggcattgg acggcatttg cggggatttt gagacaatct
     4621 ttcatgttct atggagccgg ataccaagta gaaaaagtga tttctcatcc aaattatgac
     4681 tccaagacca agaacaatga cattgcgctg atgaagctgc agaagcctct gactttcaac
     4741 gacctagtga aaccagtgtg tctgcccaac ccaggcatga tgctgcagcc agaacagctc
     4801 tgctggattt ccgggtgggg ggccaccgag gagaaaggga agacctcaga agtgctgaac
     4861 gctgccaagg tgcttctcat tgagacacag agatgcaaca gcagatatgt ctatgacaac
     4921 ctgatcacac cagccatgat ctgtgccggc ttcctgcagg ggaacgtcga ttcttgccag
     4981 ggtgacagtg gagggcctct ggtcacttcg aagaacaata tctggtggct gataggggat
     5041 acaagctggg gttctggctg tgccaaagct tacagaccag gagtgtacgg gaatgtgatg
     5101 gtattcacgg actggattta tcgacaaatg agggcagacg gcgaattcgt cgagcaccac
     5161 caccaccacc accaccacta atagccaagc ttgtcgagaa gtactagagg atcataatca
     5221 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     5281 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     5341 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5401 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc
     5461 ttgagcctag gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt
     5521 ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc
     5581 ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc
     5641 cacagcgggg catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt
     5701 gacaaaccgt catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc
     5761 tcttcgttat taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc
     5821 gaatgggacg cgccctg
//
