LOCUS       sequence-436703-       14110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit (MoClo-position 2): 
            pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Schreiber T, Prange A, Schafer P, Iwen T, Grutzner R, Marillonnet S,
            Lepage A, Javelle M, Paul W, Tissier A
  TITLE     Efficient scar-free knock-ins of several kilobases in plants by 
            engineered CRISPR/Cas endonucleases.
  JOURNAL   Mol Plant. 2024 Mar 22:S1674-2052(24)00086-8. doi: 
            10.1016/j.molp.2024.03.013.
  PUBMED    38520090
REFERENCE   2  (bases 1 to 14110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2024 Mar 22:S1674-2052(24)00086-8. doi: 10.1016/j.molp.2024.03.013."
COMMENT     Sequence Label: pAGT9569
FEATURES             Location/Qualifiers
     source          1..14110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3684..3704
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9437..9457
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      9484..9736
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9784..9808
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9964..11112)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(11448..12036)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11528..11547)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12207..12998,12999..13067))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12830..12849
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13068..13172)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      13259..13890
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    13982..14006
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagctcaac ttttgattcg ctatttgcag tgcacctgtg gcgttcatca catcttttgt
       61 gacactgttt gcactggtca ttgctattac aaaggacctt cctgatgttg aaggagatcg
      121 aaagtaagta actgcacgca taaccatttt ctttccgctc tttggctcaa tccatttgac
      181 agtcaaagac aatgtttaac cagctccgtt tgatatattg tctttatgtg tttgttcaag
      241 catgtttagt taatcatgcc tttgattgat cttgaatagg ttccaaatat caaccctggc
      301 aacaaaactt ggagtgagaa acattgcatt cctcggttct ggacttctgc tagtaaatta
      361 tgtttcagcc atatcactag ctttctacat gcctcaggtg aattcatcta tttccgtctt
      421 aactatttcg gttaattaaa gcacgaacac cattactgca tgtagaagct tgataaacta
      481 tcgccaccaa tttatttttg ttgcgatatt gttactttcc tcagtatgca gctttgaaaa
      541 gaccaaccct cttatccttt aacaatgaac aggtttttag aggtagcttg atgattcctg
      601 cacatgtgat cttggcttca ggcttaattt tccaggtaaa gcattatgag atactcttat
      661 atctcttaca tacttttgag ataatgcaca agaacttcat aactatatgc tttagtttct
      721 gcatttgaca ctgccaaatt cattaatctc taatatcttt gttgttgatc tttggtagac
      781 atgggtacta gaaaaagcaa actacaccaa ggtaaaatac ttttgtacaa acataaactc
      841 gttatcacgg aacatcaatg gagtgtatat ctaacggagt gtagaaacat ttgattattg
      901 caggaagcta tctcaggata ttatcggttt atatggaatc tcttctacgc agagtatctg
      961 ttattcccct tcctctagct ttcaatttca tggtgaggat atgcagtttt ctttgtatat
     1021 cattcttctt cttctttgta gcttggagtc aaaatcggtt ccttcatgta catacatcaa
     1081 ggatatgtcc ttctgaattt ttatatcttg caataaaaat gcttgtacca attgaaacac
     1141 cagctttttg agttctatga tcactgactt ggttctaacc aaaaaaaaaa aaatgtttaa
     1201 tttacatatc taaaagtagg tttagggaaa cctaaacagt aaaatatttg tatattattc
     1261 gaatttcact catcataaaa acttaaattg caccataaaa ttttgtttta ctattaatga
     1321 tgtaatttgt gtaacttaag ataaaaataa tattccgtaa gttaaccggc taaaaccacg
     1381 tataaaccag ggaacctgtt aaaccggttc tttactggat aaagaaatga aagcccatgt
     1441 agacagctcc attagagccc aaaccctaaa tttctcatct atataaaagg agtgacatta
     1501 gggtttttgt tcgtcctctt aaagcttctc gttttctctg ccgtctctct cattcgcgcg
     1561 acgcaaacga tcttcaggtg atcttctttc tccaaatcct ctctcataac tctgatttcg
     1621 tacttgtgta tttgagctca cgctctgttt ctctcaccac agcaatgcag actactaccc
     1681 ctgtggatcc tccatcttct cggtctgaaa acaagaggcc tcctgctcct gctggtgatg
     1741 aaggtgctgg tcctggtaga ggtcttgttg atcctgctag acctcctaag aggccaaggc
     1801 ctgattctct tcctcttgct gctgtgtgta gacctgctac tcctccatca cctggtagac
     1861 cagaaactcc tcctactcct gatctgcctt tgtctcctag aggaacccat ggtattgcag
     1921 ctccagctgg cgaacctgaa cctggttctc ctagtctgct tgagaactat gttcctccag
     1981 ctcctgatgc tggtgacgct ggttctactc cagagcctgg ttggtctgct gttgctattc
     2041 cagatgctct gccttctcat gtgctggctg agacttttga gaggcacctt tgcggtcttt
     2101 tgaggggtgt tagaaggcct ttggatgttg agcctcttag agctaggctg ggctaccttt
     2161 tctctcttgc tactgctctt gaagaggctg gcatggtgga tagaggtgtt ggtggtcatc
     2221 ttctgaggct gtctagaagg gctgctgctg cagatcctag acctcttatg gcttttttcg
     2281 aggccgctac tcagaaccag gctgaatctc aactttgggg tctgcttaga aggggtctta
     2341 ctaccgcttc tacccttaag tggggaccta gaggaccttg ctttagccct agatggctga
     2401 agaacaacgg tgatcctagg ctggacttcc agtcatctgc tgttatgttc ggtaggacca
     2461 atgagcctgc tgctagggct cttttgttca ggtattgcgt tggcagggct gatgatagag
     2521 atgctgaagg tgatgatgct ggcaggcgtt ttgttttttg cgagcctggt gatgctcctg
     2581 cagcttctgt tcatgcttgc ggtgttcttg tggatgctca cactggtatg gtgggcgctt
     2641 ctcttgatat tcttgtgtgc cctagagata ggcacggctg ccttaatcct gctccaggta
     2701 ctcctcttag gttctacgag gttaagtgcc gggctaagta cgctttcgat cctgctgatg
     2761 caggcgatcc tgttgttgct gctcatagaa ggcttgtggc taggcgttct ccatctgatt
     2821 tcagggcttt cttgcggtct attgctaggc ctggtgtgag atactttgct cctggaagag
     2881 ttcctggtcc tgaggaagct cttgtttctg atcatgctgt gtgggctgat gcaagagccg
     2941 gtgatgagaa gagaaggtgc tctgctttgg acagggctct tgtgggtctt aattctggtg
     3001 tggcttccga tgtgctgctt ttcggagatc ctgatccaga gagaaggacc gtttctcctt
     3061 tggcttggtc atctggtgct cttgtgcaca gggaacctat cttcgctaat cctaggcacc
     3121 cgaacttcaa gcagattctg gttcaggctt acgtgctggc ttctcatttt cctgaatgcc
     3181 ctcttcatcc tcacctcgtg actttcattg gtagacatag gacccctgat gaggaaggac
     3241 tttctctgag acttgaggac gctccagctt ctgctccagc agctgttagg gctgcagctg
     3301 gtgcttctat tcttccagat caggctgttc ctgtggctct gattattacc cctgttaggg
     3361 ttgacgctgc catctacgat cttatccgta ggaactctag gctcgccttc gatgagactc
     3421 ttgctagact ttgggcttct agggctcctg cttctgatcc tgccgctgct ggcgaaactt
     3481 cttcgggtgg aggtggttct ggtggaggtg gatcaggagg aggaggttcg ggtggaggtg
     3541 gttctggtgg aggtggatca ggaggaggag gatcaggtgg aggtggttct ggtggaggtg
     3601 gatcaggagg aggaggttca ggtggaggtg gttctggtgg aggtggatca ggaggaggag
     3661 gatcaggtat ggcttctagc ccaccgaaga agaagcggaa ggtcagctgg aaaatggaca
     3721 agaagtacag cattggactt gatattggta cgaactcagt tgggtgggcc gttatcaccg
     3781 atgaatacaa ggtaccttcg aagaaattta aagtgctggg caacacagat aggcacagca
     3841 ttaagaagaa cttgatcgga gctctgctct ttgactctgg agaaaccgcg gaggcgacaa
     3901 ggcttaaacg tactgcgagg agaaggtaca ctcgcaggaa gaacagaatc tgttatctcc
     3961 aagagatctt tagcaacgag atggcgaagg taaggatttt tatgatatag tatgcttatg
     4021 tattttgtac tgaaagcata tcctgcttca ttgggatatt actgaaagca tttaactaca
     4081 tgtaaactca cttgatgatc aataaacttg attttgcagg ttgacgactc gttcttccat
     4141 cgcctcgagg aatccttcct ggtagaggaa gataagaaac acgagcgtca ccccatcttt
     4201 gggaatattg ttgacgaagt agcctatcat gaaaagtatc cgactatata ccaccttcgc
     4261 aagaagctgg tggactcaac cgataaggca gaccttcggc tcatatacct ggctctcgcg
     4321 cacatgataa agtttcgtgg ccatttcttg atcgaagggg acctcaaccc ggataactcc
     4381 gatgtggata aactgttcat tcagctcgtc caaacctaca atcagctgtt cgaggagaac
     4441 cccatcaatg catcaggtaa cattccttag ttacctttct tttctttttc catcataagt
     4501 ttatagattg tacatgcttt gagatttttc tttgcaaaca atctcaggtg tcgacgccaa
     4561 ggcaatactg tctgccagac tttcgaagtc cagacggctt gagaatctga tcgctcaatt
     4621 gccaggcgag aagaagaacg gcttgttcgg gaatctgatt gcactgtctc tgggcctcac
     4681 ccctaacttc aaaagcaact ttgacctcgc cgaggacgcg aagctgcagc tgtcaaagga
     4741 tacatacgat gatgatctgg acaatctgct cgcccaaata ggtaatcttg aaattggaac
     4801 tcttcttttg ttgtctaaac ctatcaattt ctttgcggaa atttatttga agctgtagag
     4861 ttaaaattga gtcttttaaa cttttgtagg tgatcagtat gccgacctgt tcttggctgc
     4921 caagaatctg tcagacgcta tcttgctcag tgacattctg cgggtcaaca cggagataac
     4981 caaagcgcca cttagcgcct ccatgatcaa gaggtacgac gagcatcacc aggatctgac
     5041 ccttctgaag gctttggttc gccagcaact ccccgagaag tacaaggaga ttttctttga
     5101 ccaatcgaag aatggctacg cagggtacat tgatggaggt aagttgttac ttatgattgt
     5161 tttcctctct gctacatgta ttttgttgtt catttctgta agatataaga attgagtttt
     5221 cctctgatga tattattagg tgcaagtcag gaggaattct acaaattcat caagcctatt
     5281 ctggaaaaga tggacggtac agaggagctg ctcgttaaat tgaaccgcga agatttgctt
     5341 cggaagcagc gtaccttcga caatggcagc ataccgcacc agatccacct cggtgagctg
     5401 catgctatct tgaggaggca agaggacttc tatccgttcc tgaaagacaa cagagagaag
     5461 attgaaaaga tcctcacgtt ccgcattccc tactatgtag gttagtatca tatgaagaaa
     5521 tacctagttt cagttgatga atgctatttt ctgacctcag ttgttctctt ttgagaatta
     5581 tttcttttct aatttgcctg atttttctat taattcatta ggtccactcg cacgcgggaa
     5641 ctcgcggttt gcgtggatga cacgcaaatc cgaggagact atcacgcctt ggaacttcga
     5701 agaggtcgtg gacaagggtg cgagtgcaca gtccttcatc gaaaggatga ccaacttcga
     5761 taagaatctc ccaaatgaga aagtcctgcc caagcatagt ctcctgtacg aatacttcac
     5821 ggtctacaac gagctgacga aggtgaaata tgtgacggag gggatgcgca aaccggcctt
     5881 cctgtcaggt aaatcctggt ccacactttt acgataaaaa cacaagattt taaactatga
     5941 actgatcaat aatcattcct aaaagaccac acttttgttt tgtttctaaa gtaattttta
     6001 ctgttataac aggtgagcag aagaaggcca ttgtcgatct cttgttcaaa accaatcgga
     6061 aggtcactgt gaaacagctt aaagaggact actttaagaa gatcgaatgc tttgattctg
     6121 tggaaatcag cggcgttgag gataggttca atgcctctct tggcacatac catgacctgt
     6181 tgaaaatcat caaggacaag gacttccttg acaacgagga gaacgaggac atcctcgagg
     6241 acatcgtgct gactctcacg ctgtttgagg acagagaaat gatcgaggag cgccttaaga
     6301 cttatgcgca tctgttcgat gacaaggtca tgaagcagtt gaagaggagg agatatacag
     6361 gtaagaggtc aaaaggtttc cgcaatgatc cctctttttt tgtttctcta gtttcaagaa
     6421 tttgggtata tgactaactt ctgagtgttc cttgatgcat atttgtgatg agacaaatgt
     6481 ttgttctatg ttttaggttg gggaaggctc tccaggaagc tcatcaacgg catccgcgac
     6541 aagcaatccg gcaagactat actggacttt ctcaaatccg acggttttgc gaatcggaac
     6601 ttcatgcagc ttattcacga tgactcactg accttcaaag aagatatcca gaaggcccaa
     6661 gtgtcaggtc agggcgatag ccttcacgaa cacatagcca acctggctgg atcgccagct
     6721 ataaagaagg gcatactgca gacagtgaag gttgtggatg agctggtgaa ggtaagttct
     6781 gcatttggtt atgctccttg cattttaggt gttcgtcgca cttccatttc catgaatagc
     6841 taagattttt tttctctgca ttcattcttc ttgcctcagt tctaactgtt tgtggtattt
     6901 ttgttttaat tattgctaca ggtcatgggc cgccataagc cggagaacat cgtcatcgag
     6961 atggcgaggg aaaaccagac gactcagaaa gggcagaaga actcacggga gcgcatgaag
     7021 cggatagagg aaggcatcaa ggagcttggg agtcagattc tgaaagagca cccagtcgaa
     7081 aatactcaac tccagaacga gaagctgtac ctctattacc tccagaatgg gagagatatg
     7141 tacgtcgacc aagagctcga cattaacaga ctctccgact atgatgtgga tcacattgtc
     7201 cctcaatctt tcctgaagga cgatagtatt gacaacaagg taaagcaact gtgttttaat
     7261 caatttcttg tcaggatata tggattataa cttaattttt gagaaatctg tagtatttgg
     7321 cgtgaaatga gtttgctttt tggtttctcc cgtgttatag gtccttacgc gctcagacaa
     7381 gaaccgcgga aaatccgaca atgtacccag cgaggaggtt gtgaagaaga tgaagaacta
     7441 ttggaggcag cttttgaatg ctaagctcat aacccaacgg aaattcgaca atctcacgaa
     7501 ggcagaaagg ggcggactgt ctgagctcga caaagccggc ttcatcaagc gccagttggt
     7561 tgaaactcgt cagattacga aacatgtggc ccagatactc gattcgcgta tgaatacgaa
     7621 gtatgatgag aatgacaaac ttatcaggga ggtaaaggta aagtttccaa ctttccttta
     7681 ccatatcaaa ctaaagttcg aaacttttta tttgatcaac ttcaaggcca cccgatcttt
     7741 ctattcctga ttaatttgtg atgaatccat attgactttt gatggttacg caggtgatca
     7801 ccctcaagag caaactggtt agtgacttcc ggaaggactt ccagttttac aaggttcgcg
     7861 agatcaacaa ctaccatcat gcccatgacg cctacctgaa cgccgttgtt ggcactgctc
     7921 tcatcaagaa gtatccgaaa ctggagtctg agtttgtgta cggggattac aaggtgtacg
     7981 acgttaggaa gatgatcgcg aagtcagaac aagagatcgg caaggctacc gcgaaatact
     8041 tcttttactc gaatatcatg aacttcttca agacagagat cactctggcg aatggtgaaa
     8101 tccggaagag gcctctgatc gagacaaatg gcgaaacagg tctgtctttc ctatttcata
     8161 tgtttaatcc taggaatttg atcaattgat tgtatgtatg tcgatcccaa gactttcttg
     8221 ttcacttata tcttaactct ctctttgctg tttcttgcag gtgagattgt ctgggataag
     8281 ggcagggatt ttgcgactgt gcgtaaggtt ctcagcatgc cccaagtcaa catagtcaag
     8341 aaaacggagg ttcaaaccgg tggtttctcc aaggagtcca ttctccctaa gcgcaactcc
     8401 gacaaactga ttgcgaggaa gaaggattgg gatccgaaga aatacggagg ctttgatagc
     8461 cctaccgtgg catacagcgt actggtagtg gccaaggtgg agaagggcaa gagcaagaaa
     8521 ctgaaaagcg tcaaggaact gcttggaatt accataatgg aaaggtcctc gttcgagaag
     8581 aatccgatcg acttcctcga ggctaaaggt aaaatattgg atgccagacg atattctttc
     8641 ttttgatttg taactttttc ctgtcaaggt cgataaattt tatttttttt ggtaaaaggt
     8701 cgataatttt tttttggagc cattatgtaa ttttcctaat taactgaacc aaaattatac
     8761 tttgcaggtt acaaagaggt gaagaaagac ctcattatca aactgcccaa gtattcgctt
     8821 ttcgaattgg aaaatggcag aaaacgcatg ctggcatctg ccggagaact gcagaagggc
     8881 aacgagctgg cattgcccag taagtacgtc aacttcctgt acttggcctc acactatgag
     8941 aagctgaagg ggtcaccaga ggacaacgag cagaagcagt tgtttgtcga gcagcacaag
     9001 cactatcttg atgagatcat agagcagatc agcgaatttt ccaagcgggt cattcttgca
     9061 gacgctaacc tcgataaggt aaggacttct catgaatatt agtggcagat tagtgttgtt
     9121 aaagtctttg gttagataat cgatgcctcc taattgtcca tgttttactg gttttctaca
     9181 attacaggtg ctttccgcgt acaacaagca cagagataag ccgataaggg aacaagcgga
     9241 aaacatcatc cacctgttca cactgaccaa tctgggagcc ccagcagcct ttaagtactt
     9301 cgataccact atcgacagaa agcgctacac atcaaccaag gaagtgttgg acgctaccct
     9361 tattcaccaa tctattacag ggctctatga gacaaggata gatctgtcgc agttgggtgg
     9421 tgactctagg gctgacccaa agaagaagcg taaagtcggt tcgtgatcct ctagagtcaa
     9481 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     9541 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     9601 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     9661 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     9721 tctatgttac tagatcgacg ctactattgt cttctgcacg aagtggttta aactatcagt
     9781 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     9841 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     9901 acagggttcc ccagatcagg cgctggctgc tgaaccccca gccggaactg accccacaag
     9961 gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct
    10021 gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga
    10081 atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga
    10141 gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat
    10201 gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg
    10261 gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga
    10321 ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag
    10381 gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag
    10441 ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac
    10501 ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt
    10561 cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt
    10621 gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc
    10681 cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg
    10741 tttcagcaac gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt
    10801 ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc
    10861 tgccgcctcc tgttcaagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc
    10921 agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga
    10981 gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc
    11041 ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa
    11101 cgtccgattc attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc
    11161 ttattcctga tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat
    11221 gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc
    11281 ctgcacgaat accagcgacc ccttgcccaa atacttgccg tgggcctcgg cctgagagcc
    11341 aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca
    11401 catctaggat ctgccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    11461 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    11521 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    11581 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    11641 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    11701 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    11761 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    11821 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    11881 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    11941 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    12001 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    12061 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    12121 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    12181 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    12241 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    12301 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga accacgctca
    12361 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    12421 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    12481 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    12541 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    12601 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    12661 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    12721 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    12781 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    12841 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    12901 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    12961 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    13021 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    13081 caatattatt gaagcattta tcaaggttat tgtctcatga gcggatacat atttgaatgt
    13141 atttagaaaa ataaacaaat aggggttccg cgcacgaatt ggccagcgct gccatttttg
    13201 gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag
    13261 cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac
    13321 agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag
    13381 acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct
    13441 gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca
    13501 agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc
    13561 agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg
    13621 ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc
    13681 tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat
    13741 ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc
    13801 gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca
    13861 gcggcgaggg caaccagccc ggtgagcgtc gcaaaggaga tcctgatctg actgatgggc
    13921 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
    13981 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
    14041 ggacgttttt aatgtactgg ggtggatgca gtgggcccca ctctgtgaag acaagcaaga
    14101 attcaagctt
//
