LOCUS       sequence-436704-        5937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5937)
  TITLE     SGC-2024-Q1
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TMPRSS3:72-454_CO
FEATURES             Location/Qualifiers
     source          1..5937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..468
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(101..120)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     310..331
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        494..598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(599..667,668..1459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(817..836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1630..2218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2119..2138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2372..2389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2523..2747
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2814..3347)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3517..3522,3523..3539,3540..3545))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3897..3988
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3913..3930
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     4044..4106
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             4533..4547
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             5274..5297
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     polyA_signal    5439..5573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5476..5495)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5530..5549
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5602..5767
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
       61 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      121 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
      181 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
      241 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
      301 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
      361 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
      421 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca
     2461 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg
     2521 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     2581 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     2641 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     2701 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac
     2761 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg
     2821 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag
     2881 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc
     2941 gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc
     3001 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc
     3061 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg
     3121 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag
     3181 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact
     3241 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt
     3301 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     3361 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca
     3421 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     3481 gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat
     3541 gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc
     3601 agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc
     3661 acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat
     3721 ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg
     3781 accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc
     3841 caggactcta gctatagttc tagtggttgg ctacgtatac tccggaatat taatagatca
     3901 tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa
     3961 cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg
     4021 gcgcggatcc cggtcctaaa accatgaagt ttcttgtgaa tgtagcgctt gtgttcatgg
     4081 ttgtctatat tagttatata tacgccgctg ccgattgttc agggaaatat cgctgccgat
     4141 cttctttcaa gtgcatcgaa ctgatagcta gatgtgatgg tgtatcagat tgtaaagacg
     4201 gtgaggatga gtaccggtgc gtgcgagttg gaggtcagaa tgctgtattg caagtgttca
     4261 cagccgcatc gtggaaaacg atgtgctccg atgactggaa gggtcactac gcgaacgtcg
     4321 cgtgcgctca gctcggcttt ccctcctacg tatcctcaga taacttgagg gtctctagtt
     4381 tagaaggaca atttcgtgaa gagtttgtat caatcgacca cctcctgccc gacgataaag
     4441 tgaccgcact acaccattcc gtctatgttc gcgagggctg tgcttcgggc cacgtcgtaa
     4501 cactgcagtg cacggcgtgt ggtcacagac gggacgacga cgacaagata gttgggggaa
     4561 atatgtcttt gctttcacaa tggccatggc aagcatcctt acagtttcag ggctatcatc
     4621 tctgcggggg atcagtcatc acgccacttt ggattatcac tgcagcccat tgtgtgtacg
     4681 atctatacct gcccaaaagt tggactatac aggtggggct cgtgtccctg ctcgataatc
     4741 ccgcaccatc tcatttggtt gagaagatcg tatatcactc gaaatacaag ccaaaaaggt
     4801 taggaaacga tattgccctt atgaagctag ctggaccgtt gaccttcaat gagatgatcc
     4861 aaccggtttg tctgcctaac tcggaagaaa atttcccgga cggaaaggta tgttggacta
     4921 gcggctgggg cgcgactgag gatggagcag gtgatgccag tcccgtttta aaccatgccg
     4981 cagttcctct aattagcaac aaaatctgca atcatagaga tgtctatggc gggattataa
     5041 gtcctagcat gctttgcgct ggttatttaa ccgggggggt cgactcgtgt caaggggaca
     5101 gcggtgggcc tctcgtttgc caagagcgcc gtttgtggaa actagtgggc gcgacatcgt
     5161 ttggcatagg ttgtgcggag gtgaacaaac cgggagtata cacaagggtc accagctttc
     5221 tagactggat tcacgaacag atggaacgag acttaaagac ggaattcgta gaacatcacc
     5281 atcaccatca ccatcactga tgaggtacca agcttgtcga gaagtactag aggatcataa
     5341 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5401 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5461 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5521 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac
     5581 tgcttgagcc taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca
     5641 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     5701 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     5761 gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca
     5821 tgtgacaaac cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc
     5881 atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctg
//
