LOCUS       sequence-43672-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA1a-RFP
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    206..222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(230..236,237..253,254..259))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 cggcagcgga ccacctttgg taactttcag tttagcggtc tgggtacctt cgtacggacg
       61 accttcacct tcaccttcga tttcgaactc gtgaccgtta acggaacctt ccatacgaac
      121 tttgaaacgc atgaactctt tgataacgtc ttcgctactc gccatatgta tatctccttc
      181 ttaaaagatc ttttgaattc tgaaattgtt atccgctcac aattccacac attatacgag
      241 ccggatgatt aattgtcaac agctcatttc agaatatttg ccagaaccgt tatgatgtcg
      301 gcgcaaaaaa cattatccag aacgggagtg cgccttgagc gacacgaatt atgcagtgat
      361 ttacgacctg cacagccata ccacagcttc cgatggctgc ctgacgccag aagcattggt
      421 gcaccgtgca gtcgatgata agctgtcaaa ccagatcaat tcgcgctaac ttacattaat
      481 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      541 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
      601 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
      661 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
      721 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
      781 caacgcgcag cccggactcg gtaa
//
