LOCUS       sequence-43673-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA1c-RFP
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      152..223
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      239..266
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(294..311)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(508..527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
       61 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaag
      121 gatccaaact cgagtaagga tctccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      181 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      241 ctcaccttcg ggtgggcctt tctgcgttta tacctaggga tatattccgc ttcctcgctc
      301 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      361 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      421 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      481 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      541 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
      601 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
      661 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
      721 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
      781 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actg
//
