LOCUS       sequence-43676-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA1k-RFP
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    192..208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(216..222,223..239,240..245))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctttggtaac tttcagttta gcggtctggg taccttcgta cggacgacct tcaccttcac
       61 cttcgatttc gaactcgtga ccgttaacgg aaccttccat acgaactttg aaacgcatga
      121 actctttgat aacgtcttcg ctactcgcca tatgtatatc tccttcttaa aagatctttt
      181 gaattctgaa attgttatcc gctcacaatt ccacacatta tacgagccgg atgattaatt
      241 gtcaacagct catttcagaa tatttgccag aaccgttatg atgtcggcgc aaaaaacatt
      301 atccagaacg ggagtgcgcc ttgagcgaca cgaattatgc agtgatttac gacctgcaca
      361 gccataccac agcttccgat ggctgcctga cgccagaagc attggtgcac cgtgcagtcg
      421 atgataagct gtcaaaccag atcaattcgc gctaacttac attaattgcg ttgcgctcac
      481 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      541 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
      601 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
      661 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
      721 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
      781 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
//
