LOCUS       sequence-436768-        9749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  eIF4G protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9749)
  TITLE     Protein purification and mRNA synthesis plasmids
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOCC121-MBP-monoGFP-eIF4G
FEATURES             Location/Qualifiers
     source          1..9749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        79..170
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     95..112
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     regulatory      212..221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             224..1324
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1298..1321
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1379..1402
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(1403..1405,1406..1408,1409..2119)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1448..1469)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1709..1728)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2056..2077
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5024..5047
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5057..5074
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5216..5350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5253..5272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5307..5326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     3'UTR           5423..5795
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     primer_bind     complement(6147..6164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6257..6845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6337..6356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7016..7807,7808..7876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7639..7658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7877..7981)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8282..8304
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8296..8313
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8297..8313
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8324..8343
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8324..8342
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8953..9077
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(9115..9720)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 agctgccacc attttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
       61 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      121 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      181 attcataccg tcccaccatc gggcgcggat cgccaccatg ggcatgaaaa tcgaagaagg
      241 taaactggta atctggatta acggcgataa aggctataac ggtctcgctg aagtcggtaa
      301 gaaattcgag aaagataccg gaattaaagt caccgttgag catccggata aactggaaga
      361 gaaattccca caggttgcgg caactggcga tggccctgac attatcttct gggcacacga
      421 ccgctttggt ggctacgctc aatctggcct gttggctgaa atcaccccgg acaaagcgtt
      481 ccaggacaag ctgtatccgt ttacctggga tgccgtacgt tacaacggca agctgattgc
      541 ttacccgatc gctgttgaag cgttatcgct gatttataac aaagatctgc tgccgaaccc
      601 gccaaaaacc tgggaagaga tcccggcgct ggataaagaa ctgaaagcga aaggtaagag
      661 cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg ccgctgattg ctgctgacgg
      721 gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa
      781 cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg attaaaaaca aacacatgaa
      841 tgcagacacc gattactcca tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac
      901 catcaacggc ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt atggtgtaac
      961 ggtactgccg accttcaagg gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg
     1021 tattaacgcc gccagtccga acaaagagct ggcaaaagag ttcctcgaaa actatctgct
     1081 gactgatgaa ggtctggaag cggttaataa agacaaaccg ctgggtgccg tagcgctgaa
     1141 gtcttacgag gaagagttgg tgaaagatcc gcgtattgcc gccactatgg aaaacgccca
     1201 gaaaggtgaa atcatgccga acatcccgca gatgtccgct ttctggtatg ccgtgcgtac
     1261 tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat gaagccctga aagacgcgca
     1321 gactaattcg agctcgaaca acaacaacaa taacaataac aacaacagca gcggcagact
     1381 ggaagttctg ttccaggggc cgatggtgag caagggcgag gagctgttca ccggggtggt
     1441 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1501 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1561 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     1621 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1681 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1741 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1801 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1861 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1921 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1981 cgtgctgctg cccgacaacc actacctgag cacccagtcc aaactgagca aagaccccaa
     2041 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2101 catggacgag ctgtacaagg gatccgctgg ctccgctgct ggttctggcg cggccgcaat
     2161 gactgacgaa acagctcacc cgacacagag cgcctcgaaa caggagagtg cagctttgaa
     2221 gcagaccggc gatgaccagc aggagtccca gcagcagaga ggttatacaa actataacaa
     2281 cggaagcaac tacacccaga aaaagccata taacagtaac agaccacacc agcagagagg
     2341 aggtaaattc ggacccaacc gctataacaa cagaggaaat tataacggag gaggctcatt
     2401 tagaggaggc catatgggtg cgaactcatc aaatgtgcct tggaccggat attacaataa
     2461 ttatcctgta tactatcagc cgcaacagat ggccgcagca ggaagcgctc ctgcgaaccc
     2521 tattcctgta gaagaaaaga gtcccgtccc cacgaaaatt gaaataacaa ccaagtcagg
     2581 cgagcacctt gatctgaaag aacagcacaa ggcaaaactt caatcccaag agaggagcac
     2641 tgtttctccc caaccagaat ccaaacttaa agaaacgagc gatagtacat ctaccagtac
     2701 gccgactccc actccgtcca cgaatgatag caaagctagc agcgaagaaa atatctccga
     2761 ggcagaaaaa actcgtagga atttcatcga gcaggtaaag ctccgcaagg cggcgcttga
     2821 aaaaaagagg aaagagcaat tggagggaag ttcgggtaac aacaacatcc cgatgaagac
     2881 cactccagaa aacgtagaag aaaaaggatc agataaaccc gaggtcactg aaaaaaccaa
     2941 gcctgcagag gagaagtccg cggaacctga ggtcaaacaa gaaactcccg cagaggaagg
     3001 cgaacaaggc gagaaaggtc agattaaaga agagagtacg cctaaagttc tgactttcgc
     3061 ggaacgtctt aagctgaaga agcagcagaa ggaaagagag gaaaagacag agggtaagga
     3121 gaataaggaa gtgcctgttc aagaagagac aaagtccgcc atagaaagtg cgcctgtgcc
     3181 accttcagaa caagtaaaag aagagacgga ggttgctgag acggaacaat ccaatatcga
     3241 tgagtcagcc actaccccgg ctattccgac aaaaagcgat gaagctgagg cagaggtgga
     3301 agctgaagca ggagacgcgg gtacaaaaat cggactggaa gccgagattg agaccacaac
     3361 cgacgagact gacgatggca ccaacacagt cagtcatatt ttgaatgttc ttaaagacgc
     3421 tacgccaatt gaagatgtct tctcattcaa ttacccagaa ggtatcgaag gtccagacat
     3481 aaaatataaa aaggagcatg taaagtatac atatggccca actttcttgt tgcaattcaa
     3541 ggacaaactt aacgtcaagg ctgacgcaga atgggtccaa agtactgcta gtaaaatcgt
     3601 aattccccca ggcatgggcc gtggtaatcg cagccgcgac agtggacgtt ttggaaacaa
     3661 ctcaagccgc ggccacgact ttaggaacac ttcggtacgc aacatggatg accgcgctaa
     3721 ttcccgtacg agcagcaagc gtaggtcgaa gcgcatgaat gacgaccgca ggagcaatag
     3781 atcatacacc agccgcagag atagggaaag gggttcttat cgcaacgaag agaagcgtga
     3841 agacgacaag ccaaaggaag aagtcgctcc ccttgtaccg tcagctaaca ggtgggtacc
     3901 gaagttcaaa tcgaagaaaa ctgagaaaaa acttgcccct gatggcaaaa cggaactgct
     3961 cgataaagat gaagtggagc gcaagatgaa gtcacttttg aacaagttga ctctggagat
     4021 gtttgatgcc attagttctg aaatcctggc aatcgccaac atcagcgtat gggagacgaa
     4081 cggcgagacg cttaaggcgg taatagaaca aatctttctc aaagcgtgtg acgagccgca
     4141 ttggagctcc atgtatgctc agctgtgcgg caaagtagtg aaagaactca atccagacat
     4201 aacagatgaa accaacgagg gaaagactgg tccgaagttg gtcttgcact acctggtagc
     4261 tcgctgtcat gctgaattcg ataaaggttg gactgataag cttccgacga atgaagacgg
     4321 aaccccgctt gaacccgaga tgatgtctga ggagtactat gctgccgcgt ccgccaagag
     4381 gcgcggtctg ggacttgtcc gctttattgg cttcctctat cgtcttaacc ttcttacagg
     4441 caaaatgatg tttgaatgtt ttaggagact gatgaaggac ctcactgata gtcctagtga
     4501 ggagacgctg gaatccgttg ttgagctcct gaataccgta ggagagcagt tcgagactga
     4561 ctctttcagg accggacagg cgactcttga aggctcgcag ttgctcgact cgttgtttgg
     4621 aatcttggat aatattatac aaacagccaa gataagcagt agaataaaat ttaaacttat
     4681 agatattaag gagctcaggc acgacaaaaa ttggaatagt gacaaaaaag ataacggacc
     4741 gaaaactata caacagattc acgaggaaga agagaggcaa aggcagctta agaacaactc
     4801 taggagcaac tcaagacgca caaacaattc gtccaaccgt cacagcttcc gcagagacgc
     4861 tccgccagcc tcgaaggatt cgtttataac cacaagaact tatagccagc gcaacagcca
     4921 aagagcgcca ccgccaaagg aagaaccagc tgccccaaca agcacagcta cgaacatgtt
     4981 ctctgcgctc atgggtgagt ccgatgatga agaaggcgcg ccgctggaag ttctgttcca
     5041 ggggccgggc agcagccatc accatcacca tcacagtgga taatagaagc ttgtcgagaa
     5101 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     5161 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     5221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5341 tgtctggatc tgatcactgc ttgagcctag gagatccgaa ccagataagt gaaagcctgc
     5401 atccgcagat cttaattaat aaaacacgat acattgttat tagtacattt attaagcgct
     5461 agattctgtg cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa
     5521 tttataatct ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata
     5581 tctgaattta aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa
     5641 caagggttgt ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac
     5701 aaaacttgcc aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt
     5761 ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact
     5821 gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat aacgcttgga catatttaac
     5881 atcgggcgtg ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga
     5941 agttgcttcc gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa
     6001 cacgtccgcg atcaaatttg tagttgagct ttttgggcct catgggcctt ccgctcactg
     6061 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aacatggtca tagctgtttc
     6121 cttgcgtatt gggcgctctc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6181 gtaaagcctg gggtgcctaa tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6241 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6301 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6361 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6421 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6481 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6541 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6601 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6661 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6721 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6781 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6841 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6901 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6961 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7021 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7081 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7141 ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca ataaaccagc
     7201 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7261 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7321 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7381 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7441 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7501 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7561 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7621 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7681 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7741 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7801 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7861 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7921 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7981 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     8041 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     8101 ttataaatca aaagaataga ccgagatagg gttgagtggc cgctacaggg cgctcccatt
     8161 cgccattcag gctgcgcaac tgttgggaag ggcgtttcgg tgcgggcctc ttcgctatta
     8221 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     8281 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg acgtaatacg actcactata
     8341 gggcgaattg gcggaaggcc gtcaaggccg tgacgttaaa actattaagc catccaatcg
     8401 accgttagtc gaatcaggac cgctggtgcg agaagccgcg aagtatggcg aatgcatcgt
     8461 ataacgtgtg gagtccgctc attagagcgt catgtttaga caagaaagct acatatttaa
     8521 ttgatcccga tgattttatt gataaattga ccctaactcc atacacggta ttctacaatg
     8581 gcggggtttt ggtcaaaatt tccggactgc gattgtacat gctgttaacg gctccgccca
     8641 ctattaatga aattaaaaat tccaatttta aaaaacgcag caagagaaac atttgtatga
     8701 aagaatgcgt agaaggaaag aaaaatgtcg tggacatgct gaacaacaag attaatatgc
     8761 ctccgtgtat aaaaaaaata ttgaacgatt tgaaagaaaa caatgtaccg cgcggcggta
     8821 tgtacaggaa gaggtttata ctaaactgtt acattgcaaa cgtggtttcg tgtgccaagt
     8881 gtgaaaaccg atgtttaatc aaggctctga cgcatttcta caaccacgac tccaagtgtg
     8941 tgggtgaagt catgcatctt ttaatcaaat cccaagatgt gtataaacca ccaaactgcc
     9001 aaaaaatgaa aactgtggac aagctctgtc cgtttgctgg caactgcaag ggtctcaatc
     9061 ctatttgtaa ttattgaata ataaaacaat tataaatgct aaatttgttt tttattaacg
     9121 atacaaacca aacgcaacaa gaacatttgt agtattatct ataattgaaa acgcgtagtt
     9181 ataatcgctg aggtaatatt taaaatcatt ttcaaatgat tcacagttaa tttgcgacaa
     9241 tataatttta ttttcacata aactagacgc cttgtcgtct tcttcttcgt attccttctc
     9301 tttttcattt ttctcctcat aaaaattaac atagttatta tcgtatccat atatgtatct
     9361 atcgtataga gtaaattttt tgttgtcata aatatatatg tcttttttaa tggggtgtat
     9421 agtaccgctg cgcatagttt ttctgtaatt tacaacagtg ctattttctg gtagttcttc
     9481 ggagtgtgtt gctttaatta ttaaatttat ataatcaatg aatttgggat cgtcggtttt
     9541 gtacaatatg ttgccggcat agtacgcagc ttcttctagt tcaattacac cattttttag
     9601 cagcaccgga ttaacataac tttccaaaat gttgtacgaa ccgttaaaca aaaacagttc
     9661 acctcccttt tctatactat tgtctgcgag cagttgtttg ttgttaaaaa taacagccat
     9721 tgtaatgaga cgcacaaact aatatcaca
//
