LOCUS       sequence-43681-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS1k-RFP
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      153..224
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      240..267
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa
       61 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa
      121 ggatccaaac tcgagtaagg atctccaggc atcaaataaa acgaaaggct cagtcgaaag
      181 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      241 gctcaccttc gggtgggcct ttctgcgttt atacctaggg tacgggtttt gctgcccgca
      301 aacgggctgt tctggtgttg ctagtttgtt atcagaatcg cagatccggc ttcagccggt
      361 ttgccggctg aaagcgctat ttcttccaga attgccatga ttttttcccc acgggaggcg
      421 tcactggctc ccgtgttgtc ggcagctttg attcgataag cagcatcgcc tgtttcaggc
      481 tgtctatgtg tgactgttga gctgtaacaa gttgtctcag gtgttcaatt tcatgttcta
      541 gttgctttgt tttactggtt tcacctgttc tattaggtgt tacatgctgt tcatctgtta
      601 cattgtcgat ctgttcatgg tgaacagctt tgaatgcacc aaaaactcgt aaaagctctg
      661 atgtatctat cttttttaca ccgttttcat ctgtgcatat ggacagtttt ccctttgata
      721 tgtaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca ctgatagata
      781 caagagccat aagaacctca gatccttccg tatttagc
//
