LOCUS       sequence-43682-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS1k-RFP
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    183..199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(207..213,214..230,231..236))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctttcagttt agcggtctgg gtaccttcgt acggacgacc ttcaccttca ccttcgattt
       61 cgaactcgtg accgttaacg gaaccttcca tacgaacttt gaaacgcatg aactctttga
      121 taacgtcttc gctactcgcc atatgtatat ctccttctta aaagatcttt tgaattctga
      181 aattgttatc cgctcacaat tccacacatt atacgagccg gatgattaat tgtcaacagc
      241 tcatttcaga atatttgcca gaaccgttat gatgtcggcg caaaaaacat tatccagaac
      301 gggagtgcgc cttgagcgac acgaattatg cagtgattta cgacctgcac agccatacca
      361 cagcttccga tggctgcctg acgccagaag cattggtgca ccgtgcagtc gatgataagc
      421 tgtcaaacca gatcaattcg cgctaactta cattaattgc gttgcgctca ctgcccgctt
      481 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      541 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
      601 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
      661 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
      721 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta
      781 atggcgcgca ttgcgcccag
//
