LOCUS       sequence-43683-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB1c-GFP
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      47..118
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      134..161
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 agctctacaa ataaggatcc aaactcgagt aaggatctcc aggcatcaaa taaaacgaaa
       61 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      121 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatacct aggctacagc
      181 cgatagtctg gaacagcgca cttacgggtt gctgcgcaac ccaagtgcta ccggcgcggc
      241 agcgtgaccc gtgtcggcgg ctccaacggc tcgccatcgt ccagaaaaca cggctcatcg
      301 ggcatcggca ggcgctgctg cccgcgccgt tcccattcct ccgtttcggt caaggctggc
      361 aggtctggtt ccatgcccgg aatgccgggc tggctgggcg gctcctcgcc ggggccggtc
      421 ggtagttgct gctcgcccgg atacagggtc gggatgcggc gcaggtcgcc atgccccaac
      481 agcgattcgt cctggtcgtc gtgatcaacc accacggcgg cactgaacac cgacaggcgc
      541 aactggtcgc ggggctggcc ccacgccacg cggtcattga ccacgtaggc caacacggtg
      601 ccggggccgt tgagcttcac gacggagatc cagcgctcgg ccaccaagtc cttgactgcg
      661 tattggaccg tccgcaaaga acgtccgatg agcttggaaa gtgtcttctg gctgaccacc
      721 acggcgttct ggtggcccat ctgcgccacg aggtgatgca gcagcattgc cgccgtgggt
      781 ttcctcgcaa taagcccggc ccacgcctca tgcgctt
//
