LOCUS       sequence-43684-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB1c-GFP
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(63..69,70..86,87..92))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gtatatctcc ttcttaaaag atcttttgaa ttctgaaatt gttatccgct cacaattcca
       61 cacattatac gagccggatg attaattgtc aacagctcat ttcagaatat ttgccagaac
      121 cgttatgatg tcggcgcaaa aaacattatc cagaacggga gtgcgccttg agcgacacga
      181 attatgcagt gatttacgac ctgcacagcc ataccacagc ttccgatggc tgcctgacgc
      241 cagaagcatt ggtgcaccgt gcagtcgatg ataagctgtc aaaccagatc aattcgcgct
      301 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      361 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
      421 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc
      481 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
      541 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc
      601 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
      661 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
      721 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
      781 tgattgcgag tgagatattt atgccagcca gccag
//
